166,034 results

1. MSCV-XZ066-EGFRvIII

   Plasmid

   #20737

   Depositor

   Alonzo Ross

   Article

   Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

   Insert

   EGFRvIII (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   EGFR with a 267 amino acid deletion in the extrac…

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1-TFEB

   Plasmid

   #38119

   Purpose

   Mammalian expression of Transcription factor EB fused to EGFP

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   TFEB (TFEB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted stop codon

   Promoter

   CMV

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

   Plasmid

   #224435

   Purpose

   AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mScarlet-KASH
   U6-gRNA
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcBac1-Mb-sfGFP 150TAG

   Plasmid

   #197570

   Purpose

   Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   Pyl-tRNA (4 copies)
   

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   N150TAG

   Promoter

   CMV and U6/H1

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac1-Mb-sfGFP WT

   Plasmid

   #197569

   Purpose

   Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP WT
   Pyl-tRNA (4 copies)
   

   Tags

   V5-His6

   Expression

   Mammalian

   Promoter

   CMV and U6/H1

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_3XHA-ATP6V1B2-mNeonGreen

   Plasmid

   #228865

   Purpose

   Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V1B2 (ATP6V1B2 Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mNeonGreen

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE7-P2A-BSD

   Plasmid

   #224122

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-BSD
   

   Tags

   P2A-BSD, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ExRai-AMPKAR

   Plasmid

   #192446

   Purpose

   Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/mStayGold(c4)=UtrCH

   Plasmid

   #212020

   Purpose

   For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   UtrCH
   

   Tags

   mStayGold(c4)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEVOL-AckRS

    Plasmid

    #137976

    Purpose

    sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

    Depositor

    Wenshe Liu

    Article

    Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

    Insert

    AckRS and pylTcua
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV_3xFLAG-ATP6V0A3-mScarlet

    Plasmid

    #228867

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG and mScarlet

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-Flag-BRD4-WT

    Plasmid

    #90331

    Purpose

    expresses Wild type BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTn Donor

    Plasmid

    #236211

    Purpose

    Arabinose-inducible Tn5 InducTn-seq plasmid

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    None
    

    Expression

    Bacterial

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. p65-mNeonGreen

    Plasmid

    #127172

    Purpose

    Fluorescently tagged p65 reporter

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Insert

    GFP-RelA (RELA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-7 asyn A53T

    Plasmid

    #105727

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

    Insert

    alpha-synuclein A53T (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    A53T

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3.1-FLAG-SF3B1-WT

    Plasmid

    #82576

    Purpose

    Expresses a codon-optimized ORF of human SF3B1 (wild-type)

    Depositor

    Manoj Pillai

    Article

    Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

    Insert

    human SF3B1-WT (SF3B1 Human)
    

    Tags

    FLAG peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. T444T

    Plasmid

    #113081

    Purpose

    Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Bacterial

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-Gal3

    Plasmid

    #85662

    Purpose

    Fluorescent reporter for lysosomal damage

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    LGALS3 (LGALS3 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiGuide-Hygro-mTagBFP2

    Plasmid

    #99374

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-Lifeact-7

    Plasmid

    #54491

    Purpose

    Localization: Actin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    mCherry-Lifeact
    

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEF1a-hMLH1dn

    Plasmid

    #174824

    Purpose

    Mammalian expression of human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized)
    

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV CEPIA2mt

    Plasmid

    #58218

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA2mt
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V M339L

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCS2+HyPer7-NES

    Plasmid

    #136467

    Purpose

    Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Nuclear export signal from the HIV Rev protein

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. Arl13b-EGFP

    Plasmid

    #234844

    Purpose

    A mammalian expression plasmid encoding Arl13b with EGFP at C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Arl13b (ARL13B Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCFD5

    Plasmid

    #73914

    Purpose

    multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    dU6-3:tRNA:gRNA:tRNA:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. SrtA-PDGFR Tm

    Plasmid

    #121168

    Purpose

    pMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR Tm

    Depositor

    Gabriel Victora

    Article

    Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

    Insert

    SrtA-PDGFR
    

    Use

    Retroviral

    Tags

    Tomato and flag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAVS1_Puro_hPGK1_EGFP_Donor

    Plasmid

    #178088

    Purpose

    HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pT7-7 asyn E46K

    Plasmid

    #105728

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    E46K

    Promoter

    T7

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7-7 asyn G51D

    Plasmid

    #105747

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

    Insert

    alpha-synuclein G51D (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    G51D

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AttB_mCherry

    Plasmid

    #171598

    Purpose

    Strong mCherry fluorescence from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Insert

    mCherry
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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32. pSLQ14566

    Plasmid

    #239272

    Purpose

    Expresses TRF1-PYL1 for recruiting RNA to the telomere via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    TRF1-BFP-PYL1 (TERF1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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33. pSLQ10539

    Plasmid

    #239270

    Purpose

    Expresses PYL1-DDX6 for recruiting RNA to the p-body via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PYL1-BFP-DDX6 (DDX6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSLQ10531

    Plasmid

    #239267

    Purpose

    Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell lines

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    NLS-dPspCas13b-EGFP-ABI
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag tag and V5 tag

    Expression

    Mammalian

    Mutation

    H133A and H1058A in PspCas13b

    Promoter

    EF1a

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-hIBA1a-GFP-miR124T

    Plasmid

    #214147

    Purpose

    AAV vector to restrict GFP expression in microglia.

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Has Service

    AAV5

    Insert

    GFP
    

    Use

    AAV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLminP_Luc2P_RE1

    Plasmid

    #90335

    Purpose

    NFkB - p50/p65 pathway gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    NFKB

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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37. pAAV SCH9

    Plasmid

    #212708

    Purpose

    A SCHEMA evolved AAV variant effective at targeting subventricular NSCs

    Depositor

    David Schaffer

    Article

    Ojala et al Mol Ther. 2018 Jan 3;26(1):304-319. doi:

    Insert

    AAV2 Rep SCH9 Cap
    

    Use

    AAV

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Thy1.1-G5

    Plasmid

    #234839

    Purpose

    pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

    Depositor

    Gabriel Victora

    Article

    Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

    Insert

    Thy1.1 (Thy1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-P2A-5xGly

    Expression

    Mammalian

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge