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163,486 results

Showing: 1601 - 1650 of 163486 results

  3. pYPQAT43

    Plasmid
    #223415
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR; T-dna vector
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223415-plasmid-map-sequence-id-453575

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  4. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234436
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234436-plasmid-map-sequence-id-465719

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  7. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

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  11. TFORF2593

    Plasmid
    #142336
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BHLHE40 (BHLHE40 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142336-plasmid-map-sequence-id-397187

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  13. 6xHis-MBP-huMb3Cas12a

    Plasmid
    #174677
    Purpose
    Expresses 6xHis-MBP tagged Mb3Cas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huMb3Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174677-plasmid-map-sequence-id-346166

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  14. 6xHis-MBP-huMb2Cas12a

    Plasmid
    #174676
    Purpose
    Expresses 6xHis-MBP tagged Mb2Cas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huMb2Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174676-plasmid-map-sequence-id-346137

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  16. pIS1

    Plasmid
    #12179
    Depositor
    David Bartel
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12179-plasmid-map-sequence-id-234698

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  19. pLXRN-EGFP

    Plasmid
    #99207
    Purpose
    EGFP expressing bicistronic retroviral vector
    Depositor
    Peter Pedersen
    Insert
    EGFP
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    A Kozak sequence was incorporated to enhance tran…
    Promoter
    LTR
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99207-plasmid-map-sequence-id-190957

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  22. pAAV-FLEX-tdTomato

    Plasmid
    #28306
    Depositor
    Edward Boyden
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available Since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    28306-plasmid-map-sequence-id-162239

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  23. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid
    #176836
    Purpose
    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-
    Insert
    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    Use
    Unspecified
    Tags
    Expression
    Mutation
    See Depositor Comments Below
    Promoter
    Tet operator (tetracycline inducible expression)
    Available Since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176836-plasmid-map-sequence-id-346773

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  32. cycLuc_TEVS

    Plasmid
    #119207
    Purpose
    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with TEVs cleavage site
    Use
    Luciferase
    Tags
    6xHis and AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119207-plasmid-map-sequence-id-232048

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  33. pET-19b_MMLV-RT

    Plasmid
    #153312
    Purpose
    Bacterial expression of MMLV reverse transcriptase
    Depositor
    Drew Endy, Philippa Marrack
    Insert
    MMLV Reverse Transcriptase
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153312-plasmid-map-sequence-id-298742

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  34. pJA54 HLA-A*03:01

    Plasmid
    #180451
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*03:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    180451-plasmid-map-sequence-id-355758

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  35. mCherry2-C1

    Plasmid
    #54563
    Purpose
    Localization: C1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54563-plasmid-map-sequence-id-310151

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  37. pHdzCas9-KRAB

    Plasmid
    #92341
    Purpose
    Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance
    Depositor
    I-Son Ng
    Article
    Kao et al Bioresour Technol. 2017 May 4. pii: S096
    Type
    Empty backbone
    Use
    CRISPR; Plant expression
    Tags
    KRAB
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S
    Available Since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    92341-plasmid-map-sequence-id-181654

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  38. p5E-mitfa2.1

    Plasmid
    #81234
    Purpose
    zebrafish mitfa promoter entry vector for multisite Gateway cloning
    Depositor
    James Lister
    Insert
    mitfa promoter (mitfa Zebrafish)
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    mitfa, 2.1kb including 5' UTR
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    81234-plasmid-map-sequence-id-157888

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  46. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131461
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131461-plasmid-map-sequence-id-305184

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  47. pEBB-SBP-HA

    Plasmid
    #36100
    Depositor
    Ezra Burstein
    Type
    Empty backbone
    Use
    Tags
    HA and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    36100-plasmid-map-sequence-id-44719

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  48. TFORF2494

    Plasmid
    #142112
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZBTB18 (ZBTB18 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142112-plasmid-map-sequence-id-395719

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Showing: 1601 - 1650 of 163486 results