163,577 results

1. Active Wnt10B-V5

   Plasmid

   #43823

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   WNT10B (WNT10B Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOCC65

   Plasmid

   #118865

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease, mGFP

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgPten#2/Cre

   Plasmid

   #173646

   Purpose

   Expresses a Pten-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAB744

   Plasmid

   #176579

   Purpose

   Expressing the whole n-butanol pathway.

   Depositor

   Arpita Bose

   Article

   Bai et al Commun Biol. 2021 Nov 3;4(1):1257. doi:

   Insert

   phaJ, ter, adhE2, phaA, phaB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNuc-TrxRFP1

   Plasmid

   #98998

   Purpose

   mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

   Depositor

   Huiwang Ai

   Article

   Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

   Insert

   TrxRFP1 and nuclear localization sequence
   

   Use

   Tags

   His-tag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBsVchCAST-pyrE

   Plasmid

   #203813

   Purpose

   Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pgRNA-CGG

   Plasmid

   #108248

   Purpose

   Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   sgRNA targeting GGCGGCGGCGGCGGCGGCGG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-FLEX-Chrimson-TdTomato

   Plasmid

   #209202

   Purpose

   Cre-dependent expression of mCherry-tagged Chrimson; Optogenetics

   Depositor

   Larry Zweifel

   Article

   Soden et al Nat Neurosci. 2020 Aug;23(8):968-980. do

   Insert

   Chrimson
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. shPBRM1#32-Tet-pLKO-puro

   Plasmid

   #107399

   Purpose

   Lentiviral expression of shRNA targeting PBRM

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   shRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMALp2x-CARP-1

    Plasmid

    #41727

    Depositor

    Koji Okamoto

    Article

    Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    clam ADP-ribosylating protein-1
    

    Use

    Bacterial subcloning

    Tags

    Expression

    Mutation

    deleted 5'-UTR nucleotides 1-64

    Promoter

    pMB1

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

12. LifeAct-EGFP-APEX2 in pCSDEST2

    Plasmid

    #170523

    Purpose

    Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE

    Depositor

    Rob Parton

    Article

    Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

    Insert

    Cavin4 (Cavin4 Mouse)
    

    Use

    Tags

    APEX2-P2A-mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINK-OTUD4 (OTU, aa 22-196)

    Plasmid

    #61413

    Purpose

    Expresses human OTUD4 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-21 and aa 197-1114.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCerulean-Nucleus-12

    Plasmid

    #54556

    Purpose

    Localization: Nucleus, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mCerulean-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. TFORF0266

    Plasmid

    #142578

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF.DEST51-mVenus

    Plasmid

    #154899

    Purpose

    Expresses a fluorescent protein, mVenus

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-ERBB3-v2

    Plasmid

    #154894

    Purpose

    Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Tags

    Venus fragment 2 (v2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. SENP2M497A

    Plasmid

    #61088

    Purpose

    cleave the SUMO off when use PETSUMO vector for express fusion protein

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    SENP2M497A catalytic domain (SENP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M497A; Catalytic domain is residues 364-589

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pQTEV-UFD1L

    Plasmid

    #31560

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    ubiquitin fusion degradation 1-like (UFD1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJUMP47-2A(sfGFP)

    Plasmid

    #126991

    Purpose

    Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG/ATP10B-HA

    Plasmid

    #209216

    Purpose

    Mammalian expression of ATP10B

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lepr peak reporter-eGFP

    Plasmid

    #196584

    Purpose

    Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    eGFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEST-ERBB2-v1

    Plasmid

    #154901

    Purpose

    Expresses ERBB2 fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Tags

    Venus fragment 1 (v1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBbB2a-GFP

    Plasmid

    #35343

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHJ2: LexA DBD

    Plasmid

    #108240

    Purpose

    LexA DNA binding domain

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    LexA DNA binding domain (1-87)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO1

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR221-CLN3_STOP

    Plasmid

    #161034

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    CLN3 (CLN3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. Lenti-sgTp53#2/Cre

    Plasmid

    #173621

    Purpose

    Expresses a Tp53-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Tp53 (Trp53 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CVS-N2c-EGFP-FlpO

    Plasmid

    #172373

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    EGFP + FlpO
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pABM-D4-miR30-CypA

    Plasmid

    #132934

    Purpose

    Lentivector for CypA knockdown and blasticidin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    CypA shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBRC_double_attB_GFP_donor

    Plasmid

    #169692

    Purpose

    cargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)

    Depositor

    John Wang

    Article

    Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CSII-IDR2-chimpTRIM5-FOS

    Plasmid

    #79065

    Purpose

    Express chimpanzee TRIM5alpha in mammalan cells

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5alpha(chimpanzee)-FOS
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV-PS Intein

    Plasmid

    #242020

    Purpose

    AAV version of PS Intein palsmid.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein
    

    Use

    Adenoviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV GFAP CaBLAM

    Plasmid

    #244228

    Purpose

    Bioluminescent reporter for calcium signaling in astrocytes

    Depositor

    Ute Hochgeschwender

    Article

    Lambert et al bioRxiv. 2023 Jun 26:2023.06.25.546478.

    Inserts

    mNeonGreen
    CaBLAM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RAD21-HaloTag Vector

    Plasmid

    #238694

    Purpose

    Express RAD21-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    RAD21 (RAD21 Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. IL13Ra1-HaloTag BiBRET Vector

    Plasmid

    #238673

    Purpose

    Express IL13Ra1-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    IL13Ra1 (IL13RA1 Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pFUW-TetO-NFATC2

    Plasmid

    #207191

    Purpose

    doxycycline-inducible expression of Human NFATC2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

    Insert

    pFUW-TetO-NFATC2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA-DEST47-SIRT7

    Plasmid

    #242137

    Purpose

    Expression in mammalian cells

    Depositor

    J. Carl Barrett, Izumi Horikawa

    Article

    Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

    Insert

    SIRT7 (SIRT7 Human)
    

    Use

    Tags

    Cycle 3 GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV-CMV-Vcan-V3-HA

    Plasmid

    #238076

    Purpose

    Expresses Vcan V3 in mice heart tissues

    Depositor

    Yuxuan Guo

    Article

    Bai et al Cell Prolif. 2025 Jun 3:e70070. doi: 10.

    Insert

    Vcan (Vcan Mouse)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA5/FRT/TO_TOM20-HaloTag7_T2A_mEGFP

    Plasmid

    #187072

    Purpose

    Mitochondria surface expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

    Insert

    TOM20-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge