Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

50+ requests Clear filter

Applied Filters

50+ requests Clear filter

9,054 results

Showing: 2101 - 2150 of 9054 results

  1. dCas9-hHDAC3

    Plasmid
    #98591
    Purpose
    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.
    Depositor
    Zhaolan Zhou
    Article
    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10
    Insert
    dCAS9-HDAC3 (HDAC3 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    98591-plasmid-map-sequence-id-339879

    Enlarge

  4. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid
    #135391
    Purpose
    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.
    Depositor
    Fan Wang
    Insert
    TeLC-P2A-EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    135391-plasmid-map-sequence-id-266854

    Enlarge

  5. hGli1 flag3x

    Plasmid
    #84922
    Purpose
    mammalian expression of Gli1
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli1 (GLI1 Human)
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84922-plasmid-map-sequence-id-422144

    Enlarge

  6. pUC57-mitoLbNOX

    Plasmid
    #74448
    Purpose
    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Insert
    mitoLbNOX
    Tags
    Flag tag with linker and mitochondrial targeting …
    Mutation
    Human codon optimized
    Available Since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    74448-plasmid-map-sequence-id-203966

    Enlarge

  7. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

    Enlarge

  13. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

    Enlarge

  17. hCas9_D10A

    Plasmid
    #41816
    Purpose
    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Insert
    Cas9_D10A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A nickase
    Promoter
    CMV
    Available Since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    41816-plasmid-map-sequence-id-116715

    Enlarge

  19. pBabe-Kras Wt

    Plasmid
    #75282
    Purpose
    Expresses human Kras wild-type
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    None. Wild-Type
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    75282-plasmid-map-sequence-id-299863

    Enlarge

  20. pLenti-EGFP-2xFYVE

    Plasmid
    #136996
    Purpose
    Lentiviral EGFP lipid sensor for PI(3)P
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-2xFYVE
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    EF1 alpha
    Available Since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    136996-plasmid-map-sequence-id-270659

    Enlarge

  23. pcDNA3.1-Clover-mRuby2

    Plasmid
    #49089
    Purpose
    Clover-mRuby2 tandem fusion for FRET calibration
    Depositor
    Kurt Beam
    Insert
    Clover-mRuby2 fusion
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    49089-plasmid-map-sequence-id-114654

    Enlarge

  24. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

    Enlarge

  40. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid
    #29663
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, GFP, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29663-plasmid-map-sequence-id-197189

    Enlarge

  42. pYPQ203 (pMDC32-Ubi1)

    Plasmid
    #86207
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromycin resistance marker
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Expression
    Plant
    Promoter
    Maize ubiquitin 1 (ZmUbi1)
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86207-plasmid-map-sequence-id-174284

    Enlarge

  45. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

    Plasmid
    #28289
    Purpose
    3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)
    Depositor
    Alex Chenchik, Gus Frangou
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    April 20, 2011
    Availability
    Academic Institutions and Nonprofits only
    28289-plasmid-map-sequence-id-197795

    Enlarge

  50. Halo-TOMM20-N-10

    Plasmid
    #123284
    Purpose
    Localization: Outer Mitochondrial Membrane
    Depositor
    Kevin McGowan
    Insert
    TOMM20 (TOMM20 Human)
    Tags
    HaloTag7
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    123284-plasmid-map-sequence-id-242688

    Enlarge

Showing: 2101 - 2150 of 9054 results