19,158 results
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Plasmid#170446PurposeLentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.DepositorInsertmCherry-GFP-LC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-myr-HA-Akt1
Plasmid#46969Purposelentiviral expression of myr-HA-Akt1DepositorInsertAKT1 (AKT1 Human)
UseLentiviralTagsHA and myrExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-shRNA-ctrl
Plasmid#85741PurposeshRNA ctrlDepositorInsertshRNA ctrl
UseAAVTagsEYFPExpressionMutationPromoterAvailable sinceApril 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_eGFP
Plasmid#26822DepositorInsert5'UTR-eGFP-3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
R5 EMCV
Plasmid#51733PurposeDicistronic vector with EMCV IRESDepositorInsertEMCV IRES
UseTagsExpressionMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R
Plasmid#58978Purposeexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
UseTagsExpressionBacterialMutationPromoterT7/Sp6Available sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXPR_120
Plasmid#96917Purposefor CRISPRa, lentiviral expression of dCas9-VPR 2A BlastRDepositorInsertdCas9
UseLentiviralTagsVPRExpressionMammalianMutationD10A, N863APromoterEF1aAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSin Capsid
Plasmid#127693PurposePlasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Capsid
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVITRO-HPV18 L1L2
Plasmid#52592PurposeExpresses HPV18 L1 and L2 in mammalian cellsDepositorInsertsHPV18 L1
HPV18 L2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLIB
Plasmid#80610PurposepLIB - Library vector (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_R280K
Plasmid#22933DepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsv5ExpressionMammalianMutationR280K mutantPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pM-Cas12a
Plasmid#196291Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GPDepositorInsertFull length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
UseCRISPRTagsFlagExpressionPlantMutationSee depositor comments belowPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GW1-pHRed
Plasmid#31473Purposegenetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeimaDepositorInsertpHRed
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-NONO
Plasmid#127655PurposeOver-express HA-tagged human NONODepositorInsertNONO (NONO Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Oct3/4
Plasmid#13366PurposeRetroviral expression of mouse Oct3/4DepositorInsertPOU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSL2680
Plasmid#85581PurposeReplicating base vector for constructing cpf1/CRISPR editing plasmidsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-IQGAP1
Plasmid#30112DepositorInsertIQGAP1 (IQGAP1 Human)
UseTagstandem EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-hTERT
Plasmid#1773PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFR_HCV_xb
Plasmid#11510DepositorInsertFirefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
Halo-Rab6A
Plasmid#190171PurposeExpresses HaloTag-Rab6A in mammalian cells.DepositorInsertHaloTag-Rab6A
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP720-N1
Plasmid#136560PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720DepositorInsertmiRFP720
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC189
Plasmid#32114DepositorTypeEmpty backboneUseTransposon delivery vectorTags3x FLAGExpressionMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBE45
Plasmid#174655PurposeCAPTURE method receiver plasmid for Streptomyces heterologous expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ4M_hnRNPA2_FL_WT
Plasmid#139109PurposeExpress FL hnRNPA2 with a C-terminal MBP tagDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-GFP [N86/38.1R]
Plasmid#114492PurposeMammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.DepositorInsertanti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBE44
Plasmid#174654PurposeCAPTURE method 15A origin of replication receiver plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOpen-MMLV_RT (mut H)
Plasmid#165546PurposeSingle 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.DepositorInsertMoloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-Expotin 1
Plasmid#42047DepositorInsertExportin-1 (XPO1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
UBC plasmid
Plasmid#169746PurposeUnregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_Capture1_APOBEC_HA_P2A_mRuby
Plasmid#183901PurposeInducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTADepositorTypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsCapture1_APOBEC_HA_P2A_mRubyExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 hSCN5A
Plasmid#145374Purposeexpresses human Nav1.5 (WT) in mammalian cellsDepositorInsertsodium voltage-gated channel alpha subunit 5 (SCN5A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYD1
Plasmid#73447Purposeexpression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cellsDepositorTypeEmpty backboneUseTags6xHis, AGA2, T7 peptide, V5 tag, and Xpress tagExpressionYeastMutationPromoterGAL1Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p37-2iDMD-LR
Plasmid#88892Purpose37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pS-cr:GFP
Plasmid#196295Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSsDepositorInsertFull length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
UseCRISPRTagsSpeI-DR-BsaI-DR-SpeIExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP shRNA tet pLKO puro
Plasmid#163017PurposeControl tet-inducible shRNA targeting eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
GAPDH-CPG-TV
Plasmid#70148Purposea targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyADepositorInsert5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
UseMouse Targeting and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT525
Plasmid#131392PurposeL1RP retrotransposition reporterDepositorInsertL1RP GFP-AI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7pol
Plasmid#59926PurposeExpression vector for T7 RNA polymerase geneDepositorInsertT7 RNA polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-UPRT-T2A-RFP-IRES-CD
Plasmid#126677PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFPDepositorInsertUPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP
Plasmid#89684PurposepCAG promoter driving EGFPDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-AAVR-FLAG
Plasmid#166716PurposeVector expressing AAVR (KIAA0319L) with a C-terminal Flag tagDepositorInsertAAVR-FLAG (KIAA0319L Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208 CMV sTurboID (C)-HA-FRB-ERM
Plasmid#153007PurposeExpresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membraneDepositorInsertsTurboID (C) (G74)-ERM (Cb5)
UseLentiviralTagsFRB and HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_R249S
Plasmid#22935DepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationR249S MutantPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
L1-neo-TET
Plasmid#51284Purposeexpresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassetteDepositorInsertL1RE1 (L1RE1 Human)
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVS1-VIR2
Plasmid#134745PurposeVirulence and replication helperDepositorInsertvir gene cluster
UseVir gene expressionTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pTriEx4-Cdc42-2G
Plasmid#68814PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)DepositorInsertCdc42 activation reporter
UseTagsHis and MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-hPdcd4
Plasmid#20693DepositorInsertProgrammed Cell Death Protein 4 (PDCD4 Human)
UseTagsGSTExpressionBacterialMutationI36V- does not alter functionPromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only