8,626 results

1. pJT305 Pa01 Lenti pEF-attB LSR GFP

   Plasmid

   #184948

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Michael Bassik, Lacra Bintu, Patrick Hsu, Ami Bhatt

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01 Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAF0687 Pa01_attP-mCherry donor

   Plasmid

   #193466

   Purpose

   Promoterless mcherry donor for Pa01

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-lyso-pHoenix

   Plasmid

   #70112

   Purpose

   Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse, Rat)
   

   Use

   Tags

   Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBbA5c-MevT(CO)-MBIS(CO, ispA)

   Plasmid

   #35151

   Depositor

   Jay Keasling, Taek Soon Lee

   Article

   Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

   Insert

   AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   R364S in Mevalonate Kinase

   Promoter

   LacUV5,

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-U6-sgRNA-PGK-puromycin

   Plasmid

   #51133

   Purpose

   expresses sgRNA from the U6 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEIGW roGFP2-Orp1

   Plasmid

   #64993

   Purpose

   Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFP-E-Syt1

   Plasmid

   #66830

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt1 (ESYT1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-PE6a

   Plasmid

   #207851

   Purpose

   Mammalian expression of PE6a prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6a
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-SARS2-Spike

   Plasmid

   #145032

   Purpose

   Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 Spike (S Synthetic)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Ezh2[FL]-dCas9

    Plasmid

    #100086

    Purpose

    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    14056 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[FL]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCytERM_mScarlet_N1

    Plasmid

    #85066

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    CytERM
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZac2.1 gfaABC1D-tdTomato

    Plasmid

    #44332

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al J Gen Physiol. 2013 Apr 15.

    Has Service

    AAV5

    Insert

    tdTomato
    

    Use

    AAV; Adeno-associated virus ()

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FAS promoter luciferase

    Plasmid

    #8890

    Depositor

    Bruce Spiegelman

    Article

    Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

    Insert

    Fatty Acid Synthetase Promoter (Fasn unknown - rat or mouse, Rat)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmScarlet_peroxisome_C1

    Plasmid

    #85063

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Peroxisomal targeting sequence
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET263-pUC57 24xMS2V7

    Plasmid

    #140705

    Purpose

    MS2V7 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V7
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    24 MS2 loops V7, high-affinity C-variant, inter-s…

    Promoter

    None

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBB535

    Plasmid

    #27392

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 . 7():32.

    Insert

    dnaK, dnaJ (dnaJ, dnaK E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAct-Cas9

    Plasmid

    #62209

    Purpose

    Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti CMV TetR Blast (716-1)

    Plasmid

    #17492

    Purpose

    3rd gen lentiviral Tetracycline repressor expression vector, CMV promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Tetracycline repressor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn-GRAB_NE1h

    Plasmid

    #123309

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-DFCP1

    Plasmid

    #86746

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human DFCP1 (ZFYVE1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

    Plasmid

    #61629

    Purpose

    R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    mDII
    ntdTomato
    DII
    n3xVenus
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CFP-Parkin

    Plasmid

    #47560

    Purpose

    CFP tagged Parkin for colocalization study

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLX304 Luciferase-V5 blast

    Plasmid

    #98580

    Purpose

    Lentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mScarlet-I-LaminB

    Plasmid

    #98831

    Purpose

    In vivo visualization of the nuclear envelope (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    CytERM
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-ERBB4

    Plasmid

    #29527

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

    Plasmid

    #163056

    Purpose

    mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Grx1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid

    #105537

    Purpose

    AAV expression of Cre from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3-HA-HA-humanCMYC

    Plasmid

    #74164

    Purpose

    Mammalian expression of human cMyc

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    cMyc (MYC Human)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. miRFP670nano-pcDNA

    Plasmid

    #127443

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCherry-Centrin2-N-10

    Plasmid

    #55018

    Purpose

    Localization: Centrosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Centrin2 (CETN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLXSN16E6E7

    Plasmid

    #52394

    Purpose

    The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

    Depositor

    Denise Galloway

    Article

    Halbert et al J Virol. 1991 Jan;65(1):473-8.

    Insert

    HPV16 E6E7
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-N-Flag-Caspase-1

    Plasmid

    #75128

    Purpose

    Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    caspase 1 (Casp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P341S in Caspase-1 (please see depositor comments…

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PL-SIN-EF1α-EGFP

    Plasmid

    #21320

    Purpose

    Control Lentiviral vector with strong EF1a promoter, expresses EGFP

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-BRD3

    Plasmid

    #65377

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD3 (BRD3 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP-SENP3

    Plasmid

    #34554

    Depositor

    Mary Dasso

    Article

    Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

    Insert

    SENP3 (SENP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV.GFAP.eGFP.WPRE.hGH

    Plasmid

    #105549

    Purpose

    AAV expression of EGFP from GFAP promoter

    Depositor

    James M. Wilson

    Has Service

    AAV5

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCRIS-PITChv2-FBL

    Plasmid

    #63672

    Purpose

    PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    EGFP-2A-PuroR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Promoterless

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MSCV-N-Flag-HA-IRES-PURO

    Plasmid

    #41033

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Type

    Empty backbone

    Use

    Retroviral; Gateway destination vector

    Tags

    Flag, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-PV-NLS-GFP

    Plasmid

    #17300

    Depositor

    Anton Bennett

    Article

    Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

    Insert

    PV-GFP-NLS (Pvalb Rat)
    

    Use

    Tags

    Myc and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

    Plasmid

    #132771

    Purpose

    Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

    Depositor

    Jan Lammerding

    Article

    Denais et al Science. 2016 Apr 15;352(6283):353-8. do

    Insert

    Cyclic GMP-AMP synthase (CGAS Human)
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    E225A D227A

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mTagBFP2-Lysosomes-20

    Plasmid

    #55308

    Purpose

    Localization: Lysosome Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    LAMP1
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCAG-dCas9-5xPlat2AflD

    Plasmid

    #82560

    Purpose

    Expression vector for Cas9 peptide array (linker length: 22aa).

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMJ922

    Plasmid

    #78312

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-EGFP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. spNW25

    Plasmid

    #173484

    Purpose

    Expresses spNW25 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW25
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. TFEB promoter-luciferase reporter

    Plasmid

    #66801

    Purpose

    Reporter for activity of TFEB gene promoter

    Depositor

    Albert La Spada

    Article

    Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

    Insert

    TFEB promoter (Tfeb Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3.1-Peredox-mCherry-NLS

    Plasmid

    #32384

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Use

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCAG-TetON-3G

    Plasmid

    #96963

    Purpose

    Mammalian expression of Clontech rtTA driven by human beta-actin promoter

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAL119-TK

    Plasmid

    #21911

    Depositor

    Maria Castro

    Article

    Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

    Insert

    HSV Type 1 thymidine kinase
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge