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169,513 results

Showing: 2951 - 3000 of 169513 results

  2. eIF2a 2

    Plasmid
    #21808
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Mutation
    S52A
    Available Since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21808-plasmid-map-sequence-id-229779

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  3. pBS L30 mRuby3-Rab18

    Plasmid
    #166431
    Purpose
    Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Insert
    Rab18
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    L30 weak promoter
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166431-plasmid-map-sequence-id-333545

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  4. hprtSAGFP

    Plasmid
    #41594
    Purpose
    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences
    Depositor
    Maria Jasin
    Article
    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.
    Insert
    SA-GFP reporter
    Use
    Mouse Targeting; Dsb repair reporter substrate
    Expression
    Mammalian
    Available Since
    Dec. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    41594-plasmid-map-sequence-id-228244

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  5. mCherry-TGNP-N-10

    Plasmid
    #55145
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    TGNP (TGOLN2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55145-plasmid-map-sequence-id-339930

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  8. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  9. Lamp1_mStayGold

    Plasmid
    #221902
    Purpose
    Expresses mStayGold fused to Lamp1
    Depositor
    Janelia Integrative Imaging
    Insert
    Lamp1_mStayGold
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    221902-plasmid-map-sequence-id-438067

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  23. pLV-mScarlet-I

    Plasmid
    #159172
    Purpose
    To express a bright monomeric red FP for labelling and detection of mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mScarlet-I
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    Codons were optimized to Human codon usage
    Promoter
    CMV
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159172-plasmid-map-sequence-id-311154

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  29. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid
    #72486
    Purpose
    Express Luc2 and tdTomato under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72486-plasmid-map-sequence-id-418875

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  37. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Available Since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122650-plasmid-map-sequence-id-437776

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  40. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Expression
    Mammalian
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91706-plasmid-map-sequence-id-183279

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  46. pLKO.1 hygro

    Plasmid
    #24150
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
    Depositor
    Bob Weinberg
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 for shRNA
    Available Since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24150-plasmid-map-sequence-id-376525

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  47. pTiGc

    Plasmid
    #78314
    Purpose
    Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).
    Depositor
    Samantha Sampson
    Article
    Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/
    Inserts
    TurboFP635
    GFP
    Use
    Synthetic Biology; Mycobacterial replicating vect…
    Expression
    Bacterial
    Promoter
    hsp60 and hsp60(ribo)
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2951 - 3000 of 169513 results