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169,365 results

Showing: 3251 - 3300 of 169365 results

  1. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112669
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Expression
    Mammalian
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112669-plasmid-map-sequence-id-229038

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  5. TFORF3054

    Plasmid
    #144530
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SALL4 (SALL4 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144530-plasmid-map-sequence-id-404230

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  7. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a / U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139462-plasmid-map-sequence-id-308474

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  9. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid
    #203350
    Purpose
    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    Nano Luciferase
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Promoter
    T7
    Available Since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203350-plasmid-map-sequence-id-399148

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  15. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Promoter
    tipA
    Available Since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    131464-plasmid-map-sequence-id-261686

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  17. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Expression
    Yeast
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  18. pHSV-ELGA1

    Plasmid
    #246926
    Purpose
    Bioluminescent endolysosomal Ca2+ indicator lower Ca2+ affinity
    Depositor
    Teresa Alonso
    Article
    Calvo et al J Cell Biol. 2025 Dec 1;224(12):e2024100
    Insert
    ELGA1 (Endo-lysosome GFP-Aequorin)
    Use
    Herpes viral vector
    Expression
    Mammalian
    Mutation
    D117A, D119A, D163A in aequorin gene
    Promoter
    IE4/5 promoter (HSV)
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246926-plasmid-map-sequence-id-492862

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  21. pCas

    Plasmid
    #62225
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgR
    Depositor
    Sheng Yang
    Article
    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii
    Has Service
    Cloning Grade DNA
    Insert
    cas9
    Use
    CRISPR; Lambda red recombinase
    Expression
    Bacterial
    Promoter
    native cas9 promoter
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    62225-plasmid-map-sequence-id-275267

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  27. pLenti.CAG.H2B-Dendra2.W

    Plasmid
    #51005
    Purpose
    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-Dendra2
    Use
    Lentiviral
    Tags
    histone2B
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51005-plasmid-map-sequence-id-490664

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  31. pAAV2/7

    Plasmid
    #112863
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap7
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    112863-plasmid-map-sequence-id-364707

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  40. pMH-Halo tag

    Plasmid
    #154144
    Purpose
    Gateway compatible mammalian expression plasmid for halo tag fusion proteins
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    Halo tag
    Expression
    Mammalian
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154144-plasmid-map-sequence-id-309445

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  44. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid
    #196720
    Purpose
    Tet-inducible expression of Cas9-T2A-TagBFP
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196720-plasmid-map-sequence-id-389756

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Showing: 3251 - 3300 of 169365 results