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161,273 results

Showing: 3301 - 3350 of 161273 results

  4. pComb3XSS

    Plasmid
    #63890
    Purpose
    3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Inserts
    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    Use
    Phage display
    Tags
    6x His, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63890-plasmid-map-sequence-id-449948

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  8. pSIJ8

    Plasmid
    #68122
    Purpose
    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression
    Depositor
    Alex Nielsen
    Article
    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103
    Inserts
    flp recombinase
    rhaS
    rhaR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rhamnose
    Available since
    Dec. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68122-plasmid-map-sequence-id-243302

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  9. pCas

    Plasmid
    #62225
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgR
    Depositor
    Sheng Yang
    Article
    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii
    Has Service
    Cloning Grade DNA
    Insert
    cas9
    Use
    CRISPR; Lambda red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native cas9 promoter
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    62225-plasmid-map-sequence-id-275267

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  10. pCDH-CMV-mCherry-T2A-Puro

    Plasmid
    #72264
    Purpose
    Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.
    Depositor
    Kazuhiro Oka
    Insert
    mCherry-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72264-plasmid-map-sequence-id-322454

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  15. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid
    #72486
    Purpose
    Express Luc2 and tdTomato under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72486-plasmid-map-sequence-id-418875

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  16. CXCR3-DuET

    Plasmid
    #213220
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR3 (CXCR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213220-plasmid-map-sequence-id-430782

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  18. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid
    #181743
    Purpose
    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    T7
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181743-plasmid-map-sequence-id-354675

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  19. pFLAG_WT MDA5

    Plasmid
    #216798
    Purpose
    Expresses wild type (wt) melanoma differentiation-associated protein 5
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 (IFIH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216798-plasmid-map-sequence-id-428831

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  24. PB-CT3G-CERP2-MG

    Plasmid
    #175503
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175503-plasmid-map-sequence-id-345096

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  25. mCherry-Beta-Catenin-20

    Plasmid
    #55001
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55001-plasmid-map-sequence-id-300582

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  30. pH2B-mNG2(11)

    Plasmid
    #206043
    Purpose
    Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.
    Depositor
    Alisa Piekny
    Article
    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7
    Insert
    H2B gene fused to the mNG2(11) fragment
    Use
    Synthetic Biology
    Tags
    H2B-mNG2(11)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    206043-plasmid-map-sequence-id-411120

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  31. Dat - EGFP - Tol2

    Plasmid
    #85418
    Purpose
    for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene
    Depositor
    Marc Ekker
    Article
    Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:
    Insert
    dopamine transporter (slc6a3 Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    dat
    Available since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only

  39. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  47. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  48. pLV-EGFP-Cre

    Plasmid
    #86805
    Purpose
    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter
    Depositor
    Amy Lasek
    Article
    Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02
    Insert
    Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP and nuclear localization signal: PKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    86805-plasmid-map-sequence-id-436570

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  49. pCW57.1_V5_TurboID_FLAG_Nt

    Plasmid
    #194072
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    V5-TurboID-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194072-plasmid-map-sequence-id-380714

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Showing: 3301 - 3350 of 161273 results