20,433 results

1. AICSDP-19:LAMP1-mEGFP

   Plasmid

   #101782

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

   Depositor

   Allen Institute for Cell Science

   Insert

   LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

   Plasmid

   #132771

   Purpose

   Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   Cyclic GMP-AMP synthase (CGAS Human)
   

   Use

   Lentiviral

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   E225A D227A

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHIV-Luciferase

   Plasmid

   #21375

   Purpose

   self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

   Depositor

   Bryan Welm

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEx-4T-1-YTHDF3

   Plasmid

   #70088

   Purpose

   Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-HA-TCF1

   Plasmid

   #40620

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   TCF7 (TCF7 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDest-565

   Plasmid

   #11520

   Purpose

   Gateway destination vector for bacterial expression; His,GST tag

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-tTRKRAB

   Plasmid

   #12249

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Insert

   TetR-KRAB fusion
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS3Clux

   Plasmid

   #55172

   Purpose

   lux-reporter vector, integration at sacA, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS185 CMV-Cre

   Plasmid

   #11916

   Purpose

   Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

   Depositor

   Brian Sauer

   Article

   Sauer et al New Biol. 1990 May . 2(5):441-9.

   Insert

   cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1his p66shc (corrected)

    Plasmid

    #32574

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBT120a17

    Plasmid

    #138510

    Purpose

    P1

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    gIII-xLuxAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 Flag C/EBPb LAP

    Plasmid

    #66979

    Purpose

    Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    C/EBP beta (Cebpb Mouse)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

    Plasmid

    #164822

    Purpose

    Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Inserts

    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    miniCMV and pGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA GFP10-ZIP

    Plasmid

    #182241

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP
    

    Use

    Tags

    split-GFP10 tripartite

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV500 A-CREB

    Plasmid

    #33371

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    CREB (Creb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET_sfCherry(1-10)_32aalinker_sfCherry(11)

    Plasmid

    #83030

    Purpose

    Bacterial expression vector for split sfCherry screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry(1-10)_32aalinker_sfCherry(11)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK24

    Bacterial Strain

    #51950

    Purpose

    broad-host range natural conjugative plasmid

    Depositor

    Farren Isaacs

    Article

    Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

    Bacterial Resistance

    Tetracycline

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pLX311 MCL1

    Plasmid

    #117729

    Purpose

    Open reading frame vector encoding MCL1

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    MCL1L (MCL1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15-UbE2D1

    Plasmid

    #15782

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
    

    Use

    Tags

    6x His, T7 tag, and Xpress tag (enterokinase clea…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Myc-E-Syt1

    Plasmid

    #66833

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt1 (ESYT1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-mSmo

    Plasmid

    #25395

    Depositor

    Philip Beachy

    Article

    Chen et al Genes Dev. 2002 Nov 1. 16(21):2743-8.

    Insert

    mouse Smoothened cDNA (Smo Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDiGi

    Plasmid

    #59323

    Purpose

    Inducible expression of dsRed and GFP in bacteria

    Depositor

    Sophie Helaine, David Holden

    Article

    Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

    Inserts

    EGFP
    dsRed
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and Plac

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCS2_IRES2_EGFP_KCNQ2 var4

    Plasmid

    #173154

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Simian CMV IE94

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSB700

    Plasmid

    #64046

    Purpose

    Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CAGGS

    Available since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIB184 (EM)

    Plasmid

    #90194

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBABE-puro-hTERT-HA

    Plasmid

    #1772

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET16b-Trx

    Plasmid

    #36944

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. beta2-spectrin-HA

    Plasmid

    #31070

    Depositor

    Vann Bennett

    Insert

    beta2-spectrin (SPTBN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA ZIP-GFP11

    Plasmid

    #182242

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP11

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    ZIP-GFP11
    

    Use

    Tags

    split-GFP11 tripartite

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28c-F30-Broccoli

    Plasmid

    #66788

    Purpose

    Expresses Broccoli in the F30 scaffold in bacterial cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Broccoli in the F30 scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLV-hSyn1-GFP

    Plasmid

    #177810

    Purpose

    Encodes a GFP driven by human synapsin I promoter

    Depositor

    Christopher Grigsby

    Article

    Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

    Insert

    CopGFP inserted behind human synapsin I promoter (SYN1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Orai1 YFP

    Plasmid

    #19756

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pShuttle-Cre-HA

    Plasmid

    #16583

    Depositor

    Bert Vogelstein

    Article

    Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

    Insert

    cre recombinase (cre P1 bacteriophage)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-22b DT 51E/148K

    Plasmid

    #11081

    Depositor

    John Collier

    Insert

    DT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    51E/148K, catalytically inactive

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEBB-XIAPmut

    Plasmid

    #11559

    Depositor

    Jon Ashwell

    Article

    Yang et al Science 2000 May 5;288(5467):874-7.

    Insert

    XIAPmut (XIAP Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed his 467 to Alanine

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

37. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV1)

    Viral Prep

    #171027-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Promoter

    EF1a

    Tags

    tdTomato

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

38. pBA2219

    Plasmid

    #38152

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

    Insert

    DsbC (dsbC E. coli)
    

    Use

    Tags

    N-terminal leader peptide (cloning artifact)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCM189-ParB1::mCherry

    Plasmid

    #87253

    Purpose

    Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB1
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pBAD/tetO

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Human Brunello kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

    Pooled Library

    #75314

    Purpose

    Human sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

41. pCDH-PGK

    Plasmid

    #72268

    Purpose

    Express gene of interest under PGK (phosphoglycerate kinase I) promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pBAtC

    Plasmid

    #78097

    Purpose

    Plant expression of Cas9 and gRNA empty backbone; Basta resistance

    Depositor

    Jin-Soo Kim

    Article

    Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NLS-HA

    Expression

    Plant

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcdna3.1 hciap1

    Plasmid

    #8311

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMXs_FLAG-NMNAT1

    Plasmid

    #133259

    Purpose

    Express FLAG-tagged NMNAT1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT1 (NMNAT1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. VN-alpha Synuclein

    Plasmid

    #89470

    Purpose

    expresses human alpha Synuclein fused to the N-terminal part of Venus

    Depositor

    Bradley Hyman, Tiago Outeiro

    Article

    Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCALNL-GFP

    Plasmid

    #13770

    Purpose

    Cre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    EGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. RGS-6xHis-BLIMP-1-pcDNA3.1-

    Plasmid

    #52518

    Purpose

    expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Blimp1 (PRDM1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAAV-Ef1a-Con/Fon-GCaMP6F (AAV8)

    Viral Prep

    #137122-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-GCaMP6F (#137122). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-GCaMP6F plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    None

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

49. pCGN N-Ras wt

    Plasmid

    #14723

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    N-ras (NRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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50. p2attPC

    Plasmid

    #51547

    Purpose

    DICE donor plasmid without neural transcription factors.

    Depositor

    Michele Calos

    Article

    Zhu et al Nucleic Acids Res. 2013 Dec 4.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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