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20,807 results

Showing: 10951 - 11000 of 20807 results

  2. C1QBP-bio-His

    Plasmid
    #53330
    Purpose
    Expresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    C1QBP (C1QBP Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53330-plasmid-map-sequence-id-83060

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  4. pAAV-SynaptoTAG2

    Plasmid
    #175275
    Purpose
    AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
    Use
    AAV
    Tags
    EGFP and tdTomato (P2A cleavage)
    Promoter
    Synapsin
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175275-plasmid-map-sequence-id-349084

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  15. STING-V2

    Plasmid
    #124264
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus2
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124264-plasmid-map-sequence-id-241461

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  16. AICSDP-42:AAVS1-mTagRFPT-CAAX

    Plasmid
    #107580
    Purpose
    Homology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cells
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    May 9, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    107580-plasmid-map-sequence-id-208159

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  17. pWS175

    Plasmid
    #90962
    Purpose
    Cas9 gap repair vector - HygR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90962-plasmid-map-sequence-id-287209

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  21. pCDNA3.3_CoV2_B.1.1.7

    Plasmid
    #170451
    Purpose
    Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Yuan et al Science. 2021 Aug 13;373(6556):818-823.
    Insert
    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
    Tags
    NA
    Expression
    Mammalian
    Mutation
    HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …
    Promoter
    CMV
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170451-plasmid-map-sequence-id-333038

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  27. GPR158-Tango

    Plasmid
    #66332
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR158 (GPR158 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pCS6-YFP-SMASh(TI)

    Plasmid
    #111501
    Purpose
    Expresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    YFP-SMASh(TI)
    Tags
    HA epitope tag and SMASh tag
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, S122R, a…
    Promoter
    CMV
    Available Since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111501-plasmid-map-sequence-id-214268

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  34. pBIR-GFP

    Plasmid
    #49807
    Purpose
    In vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.
    Depositor
    Thanos Halazonetis
    Insert
    GFP
    Expression
    Mammalian
    Promoter
    beta-actin
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49807-plasmid-map-sequence-id-74303

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  38. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available Since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108692-plasmid-map-sequence-id-208976

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  46. AICSDP-19:LAMP1-mEGFP

    Plasmid
    #101782
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1
    Depositor
    Allen Institute for Cell Science
    Insert
    LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Available Since
    Nov. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101782-plasmid-map-sequence-id-198613

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Showing: 10951 - 11000 of 20807 results