164,349 results
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Plasmid#222848PurposeE. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivityDepositorInsertAT118-L
UseTagsV5-HisExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
EphA2-MT
Plasmid#108851PurposeEncodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linkerDepositorInsertEPHA2 (EPHA2 Human)
UseTagsLabeled with mTurquoise on the C-Terminus via a 1…ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf
Plasmid#175524PurposeCo-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cellsDepositorInsertBeta4Gal-HaloTag7 and Lyn11-SNAPf
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMSCVFLAG-ZFP423
Plasmid#24764DepositorInsertZinc Finger Protein 423 (Zfp423 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgE/λ
Plasmid#61879PurposeHuman immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin Epsilon constant region
Human immunoglobulin lambda constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Annexin-12
Plasmid#53991PurposeLocalization: Plasma Membrane, Excitation: 487, Emission: 509DepositorInsertAnnexin (ANXA4 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pZS2-123
Plasmid#26598DepositorInsertSynthetic
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Mo-Trip6
Plasmid#27254DepositorInsertTrip6 (Trip6 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
BDKBR1-Tango
Plasmid#66229PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertBDKBR1 (BDKRB1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Puro-EF-1α>{H2B-RFP}
Plasmid#201121PurposeRFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclearDepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationNoPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U2B''-fSNAPc
Plasmid#106988Purposeopen reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorInsertUB2" (SNRPB2 Human)
UseTagsfSNAPExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG-V5-Snail2-IRES-nls-GFP
Plasmid#44282DepositorInsertV5-Snail2 (SNAI2 Chicken)
UseTagsV5ExpressionMammalianMutationPromoterCMV-IE-enhancer and chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
GPR_control_lenti
Plasmid#216223PurposeGFP P2A RFP control reporter in a lenti backboneDepositorInsertGFP P2A RFP_stop
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CCreV
Plasmid#140132Purposecan be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomerDepositorInsertCCreV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NCreV
Plasmid#140131Purposecan be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomerDepositorInsertNCreV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL30008
Plasmid#50300DepositorInsertN terminal HA tag (6x Human influenza hemagglutinin)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMIN-VS1
Plasmid#197783PurposeLevel 2 destination vector: pMIN-VS1 backboneDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
13XLexAop2 IVS ChrimsonR mCherry_tr
Plasmid#111548PurposeOptogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_FH_WT
Plasmid#82210PurposeGateway Donor vector containing FH , part of the Target Accelerator Plasmid Collection.DepositorInsertFH (FH Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL30009
Plasmid#50301DepositorInsertN terminal Myc tag (4x Myc)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U1-70K-fSNAPc
Plasmid#106987Purposeopen reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorInsertU1-70K (SNRNP70 Human)
UseTagsfSNAPExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1
Plasmid#85070PurposeYellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)DepositorInsertPH-PLCD1 (PLCD1 Human)
UseTagsIRES-sYFP2-PH and mScarlet-HExpressionMammalianMutationPLCD aa 1–170PromoterCMVAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-MYOD1_AroPERFECT
Plasmid#215620PurposeFor integration of MYOD1 AroPERFECT T2A mEGFPDepositorInsertMYOD1 AroPERFECT (MYOD1 Human)
UseTagsT2A mEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
FL-fibulin-3-V5-lentiviral-GFP
Plasmid#29703DepositorInsertEFEMP1 (EFEMP1 Human)
UseLentiviralTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-GFP
Plasmid#170176PurposeExpresses GFP under the control of EF1a short promoterDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-KAR2-msGFP2-HDEL
Plasmid#160468PurposeYeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signalDepositorInsertKAR2-msGFP2-HDEL
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-MCAK-C-7
Plasmid#54161PurposeLocalization: Microtubules/Kinesin, Excitation: 487, Emission: 509DepositorInsertMCAK (KIF2C Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-moxGFP-Blast-H2BC11
Plasmid#207757PurposeDonor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC25A48
Plasmid#131995PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A48 (SLC25A48 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMK1200
Plasmid#84219PurposeLentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherryDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL50010
Plasmid#50310DepositorInsertC terminal Myc tag (4x Myc)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Cx43-7
Plasmid#54055PurposeLocalization: Gap Junctions, Excitation: 487, Emission: 509DepositorInsertCx43
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDCAF3-IS5
Plasmid#65220PurposeExpresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.DepositorInsertsndmA
ndmB
ndmC
ndmD
gst9
chlR
UseSynthetic BiologyTagsExpressionBacterialMutationChanged start codon from gtg to atg and Changed s…PromoterBBa_J23100Available sinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOGG001
Plasmid#113978PurposeLevel 0 module. nptII promoter for constitutive gene expressionDepositorInsertnptII promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX317 PRDM14
Plasmid#193682PurposeConstitutive lentiviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CDS12_mTurquoise2
Plasmid#136089PurposeFP for N-term fusion with a CTAGDepositorInsertCDS12_mTurquoise2(noStop)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICSL86977OD
Plasmid#86180PurposeGG compatible CDs acceptor BASTADepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2Aneo v2
Plasmid#80033PurposeUsed to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trappingDepositorTypeEmpty backboneUseAAV and Cre/Lox; A platform vector to construct t…TagsExpressionMutationPromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC39A6_STOP
Plasmid#161319PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC39A6 (SLC39A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUPD+Red-F
Plasmid#170874PurposePhytobrick - Red-F Luciferase CDSDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionMutationp.S286Y Red mutantPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Tensin1-C-14
Plasmid#54274PurposeLocalization: Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertTensin1 (TNS1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB1A3_GFP_dT23
Plasmid#89663Purposeexpression of doubleTAL staple protein dT23 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT23
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A47
Plasmid#131983PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A47 (SLC25A47 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB1A3_GFP_dT34
Plasmid#89669Purposeexpression of doubleTAL staple protein dT34 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT34
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
SV40 Large T K1 mutant in pENTR1A (w414-1)
Plasmid#22270DepositorInsertSV40 Large T antigen TK1 mutant (E107K)
UseEntry vectorTagsExpressionMutationE107K (K1 mutation)PromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_40
Plasmid#109239PurposePTac driving TALEsp2DepositorInsertTALEsp2
UseTagsExpressionBacterialMutationPromoterPTacAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT33
Plasmid#89668Purposeexpression of doubleTAL staple protein dT33 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT33
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK_kanR_proJK1_PAD
Plasmid#60630PurposeConstitutively expresses PAD (phenolic acid decarboxylase)DepositorInsertPhenolic acid decarboxylase padC (padC )
UseTagsExpressionBacterialMutationK146YPromoterproJK1Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only