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179,463 results

Showing: 2351 - 2400 of 179463 results

  11. AIO-Puro

    Plasmid
    #74630
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Publication
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Puromycin resistant marker
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    74630-plasmid-map-sequence-id-373032

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  13. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Publication
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21 Articles
    60820-plasmid-map-sequence-id-371636

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  14. mCherry-Gamma-Tubulin-17

    Plasmid
    #55050
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Gamma-Tubulin (TUBG1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    55050-plasmid-map-sequence-id-215600

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  29. pGP-AAV-syn-jGCaMP7f-WPRE (AAV PHP.eB)

    Viral prep
    #104488-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only

  30. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (AAV1)

    Viral prep
    #75470-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (#75470). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, Flp recombinase-dependent expression of channelrhodopsin H134R mutant fused to mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Connie Cepko
    Publication
    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:
    Promoter
    CAG
    Tags
    mCherry
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  31. pLVX-puro-cOVA-IRES-BFP

    Plasmid
    #135074
    Purpose
    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.
    Depositor
    Maria Castro
    Insert
    cOVA-IRES-BFP (OVAL Chicken)
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    135074-plasmid-map-sequence-id-266791

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  32. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)

    Viral prep
    #100854-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only

  34. pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV Retrograde)

    Viral prep
    #65014-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Promoter
    Syn
    Tags
    GFP
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only

  35. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV Retrograde)

    Viral prep
    #51084-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available since
    June 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  37. pAAV-CAG-tdTomato (codon diversified) (AAV5)

    Viral prep
    #59462-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available since
    Oct. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  38. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

    Plasmid
    #64222
    Purpose
    Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    sgRNA cassette
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    May 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    64222-plasmid-map-sequence-id-107958

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  44. Zinc Finger Consortium Expression Vector and Modular Assembly Kit

    Plasmid kit
    #1000000005
    Purpose
    Plasmids and strain to assemble Zinc finger nucleases (ZFNs) for performing genome engineering in a variety of organisms.
    Depositor
    Keith Joung
    Application
    Genome Editing
    Vector type
    Bacterial Expression, Insect Expression, Mammalian Expression, Plant Expression
    Editing type
    ZFN
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  45. Zinc Finger Consortium Expression Vector Kit v1.0

    Plasmid kit
    #1000000010
    Purpose
    Plasmids and strain for the bacterial two-hybrid system used to screen Zinc finger nucleases (ZFNs).
    Depositor
    Keith Joung
    Application
    Genome Editing
    Vector type
    Bacterial Expression, Insect Expression, Mammalian Expression, Plant Expression
    Editing type
    ZFN
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV-hsyn-GRAB_5-HT1.0 (AAV9)

    Viral prep
    #140552-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_5-HT1.0 (#140552). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_5-HT1.0 plasmid DNA. Synapsin-driven expression of GRAB_5-HT1.0 sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Publication
    Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s
    Promoter
    Syn
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 2351 - 2400 of 179463 results