Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

166,819 results

Showing: 2701 - 2750 of 166819 results

  9. FU-H2B-GFP-IRES-Puro

    Plasmid
    #69550
    Purpose
    Lentiviral, selectable fluorescent GFP tagging of nuclei.
    Depositor
    Charles Gersbach
    Insert
    Histone 2B - green fluorescent protein fusion
    Use
    Lentiviral
    Tags
    GFP fusion
    Expression
    Mutation
    Promoter
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69550-plasmid-map-sequence-id-122943

    Enlarge

  10. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (AAV5)

    Viral Prep
    #100834-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (#100834). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 plasmid DNA. CamKII-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Tags
    None
    Available Since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. pT3-EF1A-MYC-IRES-lucOS

    Plasmid
    #129776
    Purpose
    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
    Depositor
    Amaia Lujambio
    Article
    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82
    Insert
    human MYC (MYC Human)
    Use
    Tags
    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    129776-plasmid-map-sequence-id-263368

    Enlarge

  14. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

    Enlarge

  18. pPSU1

    Plasmid
    #89439
    Purpose
    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Article
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89439-plasmid-map-sequence-id-190157

    Enlarge

  20. pPSU2

    Plasmid
    #89566
    Purpose
    High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Article
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    750 bp EcoRV fragment
    3000 bp EcoRV fragment
    4000 bp EcoRV fragment
    50 bp PstI fragment
    100 bp PstI fragment
    200 bp PstI fragment
    300 bp PstI fragment
    400 bp PstI fragment
    500 bp PstI fragment
    600 bp PstI fragment
    1500 bp Psti fragment
    4100 bp PstI fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89566-plasmid-map-sequence-id-177206

    Enlarge

  21. pAAV.synP.DIO.EGFP.WPRE.hGH (AAV9)

    Viral Prep
    #100043-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.synP.DIO.EGFP.WPRE.hGH (#100043). In addition to the viral particles, you will also receive purified pAAV.synP.DIO.EGFP.WPRE.hGH plasmid DNA. Synapsin-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ian Wickersham
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. HOP-flash

    Plasmid
    #83467
    Purpose
    TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene
    Depositor
    Barry Gumbiner
    Article
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Insert
    Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83467-plasmid-map-sequence-id-350804

    Enlarge

  26. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (AAV5)

    Viral Prep
    #130998-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (#130998). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie
    Promoter
    EF1a
    Tags
    mScarlet
    Available Since
    March 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  29. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV2)

    Viral Prep
    #105530-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available Since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  31. iCas

    Plasmid
    #84232
    Purpose
    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)
    Depositor
    Meng How Tan
    Article
    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038
    Insert
    Cas9
    Use
    Tags
    2A-OFP co-expression and ERT2-ERT2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    84232-plasmid-map-sequence-id-323254

    Enlarge

  34. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (AAV8)

    Viral Prep
    #130909-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (#130909). In addition to the viral particles, you will also receive purified AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. CAG-driven, Flp-dependent expression of ChrimsonR-tdTomato. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    CAG
    Tags
    tdTomato (Flp-dependent)
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  41. pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV Retrograde)

    Viral Prep
    #65014-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Promoter
    Syn
    Tags
    GFP
    Available Since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only

  44. pAAV.CAG.GCaMP6f.WPRE.SV40 (AAV1)

    Viral Prep
    #100836-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.GCaMP6f.WPRE.SV40 (#100836). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Tags
    None
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  45. pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV9)

    Viral Prep
    #50476-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  47. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid
    #174722
    Purpose
    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    eGFP
    pac
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and F2A linker
    Expression
    Mammalian
    Mutation
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174722-plasmid-map-sequence-id-348963

    Enlarge

  48. PARP1 FL

    Plasmid
    #169815
    Purpose
    Expresses codon optimised full length His tagged PARP1 in bacterial cells
    Depositor
    David Neuhaus
    Article
    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226
    Insert
    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
    Use
    Tags
    MKHHHHHHMKQ
    Expression
    Bacterial
    Mutation
    Valine 762 mutated to an alanine
    Promoter
    T7 promoter
    Available Since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    169815-plasmid-map-sequence-id-334563

    Enlarge

Showing: 2701 - 2750 of 166819 results