173,387 results
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Plasmid#70453PurposeGateway ORF clone of human PIK3CG [NM_002649.3] with stop codon (for native or N-terminal fusions)DepositorInsertPIK3CG (PIK3CG Human)
UseGateway entry cloneAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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CRISPR_TRAC
Plasmid#164993PurposeExpression of gRNA targeting TCRalpha constant locusDepositorInsertgRNA against TRAC
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYSD300
Plasmid#212930PurposeEncodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.DepositorInsertAGA1 (AGA1 Budding Yeast)
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SPNS2_STOP
Plasmid#161473PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSPNS2 (SPNS2 Human)
ExpressionMammalianAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG416GAL-ccdB-EGFP
Plasmid#14195DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS AF
Plasmid#140023PurposePlasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationY271A, Y349FPromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPIKE-P
Plasmid#101172Purposechimeric spike resembling V4 region of prokaryotic 16S rRNA to be used in metagenomicsDepositorInsertV4 16S rRNA chimeric spike
UseSynthetic BiologyAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-ZNF746
Plasmid#207825PurposeDox-inducible expression of ZNF746DepositorAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
cyto-iATPSnFR1.0
Plasmid#102550Purposecytoplasmic ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.0
ExpressionMammalianAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)
Plasmid#140659PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NlucDepositorInsertOsTIR1(F74G) mAID-EGFP-Nluc TOL2
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDG_KCNK12_OE_1
Plasmid#161632PurposeOverexpresses KCNK12DepositorAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MoClo Tool Kit
Plasmid Kit#1000000044PurposeCollection of standardized genetic modules that can be used for hierarchical assembly of multi-gene expression constructs.DepositorApplicationCloning and Synthetic BiologyVector TypeEukaryotic ExpressionCloning TypeGolden Gate (MoClo)Available SinceApril 8, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC_C_FGFR2
Plasmid#187751PurposeMAC-tagged gene expression of human FGFR2DepositorInsertFGFR2 (FGFR2 Human)
ExpressionMammalianAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0375
Plasmid#141596PurposeLentiviral vector for overexpressing the MYBL1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYSD153
Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCoofy35
Plasmid#43985DepositorTypeEmpty backboneTagsMBPExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
Affibody EGFR:1907.BirA*-pET301/NT-DEST
Plasmid#174691PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
TagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-EGFP-ccdB
Plasmid#14309DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BIRC7
Plasmid#196363Purposeprotein expression of the His-tagged full length proteinDepositorInsertBIRC7/Livin
TagsHis6-TEVExpressionBacterialPromoterT7Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSS02:cyt-Peredox-mCherry
Plasmid#161743Purposecytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plantsDepositorInsertcyt-Peredox-mCherry
ExpressionPlantPromoterUbiquitin 10Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbS15c-RFP
Plasmid#126198PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23100Available SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK 4x wt
Plasmid#11313DepositorInsert4 bulged binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
lenti CAG-FLAG-dCas9-VPR
Plasmid#114197PurposeLentivirus compatible dCas9-VPR construct driven by the CAG promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianPromoterCAGAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti_dCas9-KRAB-MeCP2
Plasmid#122205PurposeBipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.DepositorInsertdCas9-KRAB-MeCP2
UseLentiviralTagsKRAB-MeCP2ExpressionMammalianMutationCas9 (D10A, H840A)Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ERT2-iCre-ERT2
Plasmid#178059PurposeConditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.DepositorInsertERT2-iCre-ERT2
UseAAVExpressionMammalianPromoterHuman synapsin 1 (Syn) promoterAvailable SinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4(TA) + PGK-puro
Plasmid#167826PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
Tagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNarsenic
Plasmid#247320PurposeBiosensor module for arsenic in E. coli with naringenin-inducible arsR expressionDepositorInsertsfdeR
mkate2
Arsenical resistance operon repressor
UseSynthetic BiologyPromoterP_arsOC2, P_fdeA, and P_fdeRAvailable SinceMarch 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.mCherry
Plasmid#248069PurposeiATPSnFR2.S29W.A95K variant with non-responsive mCherryDepositorInsertiATPSnFR2.S29W.A95K.mCherry
UseAAVMutationS29W.A95KAvailable SinceMarch 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.mCherry
Plasmid#248073PurposeiATPSnFR2.A95A.A119L variant with non-responsive mCherryDepositorInsertiATPSnFR2.A95A.A119L.mCherry
UseAAVMutationA95A.A119LAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).cpSFGFP.tdTomato
Plasmid#248076PurposecpSFGFP control with non-responsive mIRFP670nano3DepositorInsertcpSFGFP.mIRFP670nano3
UseAAVAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
TET1cd-dCas9 (D12)
Plasmid#239488PurposeEncodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.DepositorInsertRPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-P2A-BFP
UseCRISPRExpressionPlantAvailable SinceFeb. 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSFV_SRF-NLS-VP64 (3'UTR)_RLEloop
Plasmid#240258PurposeAttenuated PROTEUS vector with SRF-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.DepositorInsertSRF
TagsNLS-VP64ExpressionMammalianMutationSRF[aa1-265]; VP64 = 4*VP16[aa437-447]PromoterSFV subgenomic promoterAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_p53-ZF43
Plasmid#240263PurposeConstitutive expression (CMV promoter) of a fusion of the p53 DNA-binding domain and ZF43. Used as part of the circuit for Nb139-VP64 transgene activity.DepositorInsertp53-ZF43
ExpressionMammalianMutationp53[aa93-393]PromoterCMVAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pZF43x6_luciferase
Plasmid#240267PurposeCircuit-responsive (ZF43RE promoter) expression of firefly luciferase. Used for evaluation of Nb139-VP64 transgene activity.DepositorInsertfirefly luciferase
UseLuciferaseExpressionMammalianPromoterZF43x6-CAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTL50_cHS4-CAG-s36GFPmNG-t2A-PuroR-p2A-BFP-polyAcHS4
Plasmid#223541PurposePUFFFIN cassette with s36GFP-mNeonGreen and tagBFP, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cellsDepositorInserts36GFP-mNeonGreen, tagBFP, PAC
ExpressionMammalianPromoterCAGAvailable SinceJan. 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.A95K.mCherry
Plasmid#248071PurposeiATPSnFR2.A95K variant with non-responsive mCherryDepositorInsertiATPSnFR2.A95K.mCherry
UseAAVMutationA95KAvailable SinceJan. 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pKG1988_pPV1-UbC
Plasmid#239689PurposeBsaI Golden Gate assemby plasmid encoding the UbC promoterDepositorInsertUbC promoter
UseSynthetic BiologyMutationRemoval of BsaI cut sitesAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 JDM37-GFPnb-mCh
Plasmid#235666PurposeExpresses designed activator of Hsp70 tagged with a GFP nano body and mCherryDepositorInsertJDM37-GFPnb-mCh (PMCH Synthetic, Human)
TagsGFP nanobody and mCherryExpressionMammalianPromoterCMVAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYSD080
Plasmid#212917PurposeEncodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA1 (AGA1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD083
Plasmid#212920PurposeEncodes the HA-tagged S. cerevisiae Sed1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSED1 (SED1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD003
Plasmid#212900PurposeEncodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertECM14 (ECM14 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD023
Plasmid#212909PurposeEncodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD081
Plasmid#212918PurposeEncodes the HA-tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD005
Plasmid#212902PurposeEncodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertKSH1 (KSH1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-ZNF746-C571A
Plasmid#207826PurposeDox-inducible expression of ZNF746-C571ADepositorAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FusionRed
Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
ExpressionYeastPromoterCUP1 promoterAvailable SinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS238D1::sptse5-CT
Plasmid#192959PurposeFor expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studiesDepositorInsertsptse5-CT
TagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1317)Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZMYND8-2A-neo
Plasmid#190619PurposeLentiviral expression plasmid of human ZMYND8, neomycin selectionDepositorInsertZMYND8 (ZMYND8 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationsynonymous mutation for sgRNA resistance at aa214…Available SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only