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pJL1-mCherry

Plasmid

#102629

Purpose

In vitro expression of mCherry from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mCherry

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pETNb-nALFA

Plasmid

#159988

Purpose

For the protein expression with ALFA tag at N-terminus in E. coli

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

Expression

Bacterial

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304-Flag-APEX2-NES

Plasmid

#92158

Purpose

Cytosolic APEX2 construct

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

Flag-APEX2-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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SpCas9-YE1-BE4max

Plasmid

#198546

Purpose

Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-HaloTag7-mGFP

Plasmid

#184903

Purpose

Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

monomeric EGFP

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNa

Plasmid

#107980

Purpose

expression of full-length OPN

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

full-length osteopontin (SPP1 Human)

Expression

Mammalian

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDMyc-TNPO1

Plasmid

#86081

Purpose

A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Tags

Myc

Expression

Mammalian

Promoter

T7

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCas9-Venus

Plasmid

#70267

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Article

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

Cas9
Venus Reporter

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS and P2A

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLX304-VHL-IRES-Tdtomato

Plasmid

#187643

Purpose

VHL Expression vector with Tdtomato

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

VHL (VHL Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAN-PTet-dCas9

Plasmid

#62244

Purpose

controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

catalytically inactive, D10A, H840A

Promoter

Ptet

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCnCre

Plasmid

#232907

Purpose

Electroporation vector for expression of Cre recombinase in Cuprividus necator

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

Cre recombinase (cre Synthetic)

Use

Cre/Lox and Synthetic Biology

Promoter

Ptac-cymO

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D hM3D mCherry SV40

Plasmid

#92284

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

hM3D (CHRM3 Human)

Use

AAV

Tags

mCherry

Promoter

GfaABC1D

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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ABE-NW1

Plasmid

#240615

Purpose

Adenine base editor with narrowed editing window

Depositor

Tingting Jiang

Article

Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

Insert

TadA8e(N108Q,M151C,Q154Y)-linker-nCas9 (D10A)

Use

CRISPR

Expression

Mammalian

Mutation

TadA8e(N108Q,M151C,Q154Y),Cas9 (D10A)

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry_pBirA-HA_FKBP8

Plasmid

#154457

Purpose

expresses mCherry_pBirA-HA_FKBP8/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry_pBirA-HA_FKBP8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.18 NR0B1-Luc

Plasmid

#100983

Purpose

NR0B1 luciferase reporter - firefly luciferase driven by NR0B1 promoter

Depositor

Lee Helman

Article

Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

Insert

NR0B1 (NR0B1 Human)

Expression

Mammalian

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-sRGECO (AAV8)

Viral Prep

#137127-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-sRGECO (#137127). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-sRGECO plasmid DNA. EF1a-driven, Cre-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Promoter

EF1a

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 puro Nodamura B2

Plasmid

#17228

Depositor

Christopher Sullivan

Article

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Expression

Mammalian

Mutation

2 silent point mutations

Available Since

June 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pWZL-Blast FAPwt

Plasmid

#210643

Purpose

Expresses the FAP (Fibroblast Activation Protein) protein.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

none

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstarC

Plasmid

#232737

Purpose

Expresses control sensor NAPstarC in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstarC

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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VP64-dCas9

Plasmid

#177171

Purpose

VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

VP64-dCas9(D10A, H840A)

Use

CRISPR

Tags

NLS and NLS-3xFLag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE_hygro_mRFP1_NRF2

Plasmid

#136579

Purpose

mammalian expression of mRFP1-tagged human NRF2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (NFE2L2 Human)

Use

Retroviral

Tags

N-mRFP1-FLAG

Expression

Mammalian

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pIB2

Plasmid

#25451

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pEJS578_DD-dSpyCas9-mCherry-APEX2

Plasmid

#108570

Purpose

Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Insert

dSpyCas9

Use

CRISPR and Lentiviral

Tags

APEX2, Destabilization domains, Flag, and mCherry…

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Sox2

Plasmid

#13367

Purpose

Retroviral expression of mouse Sox2

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

SRY-box containing gene 2 (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR4GN (Lenti)

Plasmid

#222693

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Dual sgRNA CRISPRi Library

Pooled Library

#1000000192

Depositor

Jonathan Weissman

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
pHSP02

Plasmid

#117259

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537

Use

Other

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLI50

Plasmid

#13573

Depositor

Chia Lee

Article

Lee et al Gene. 1991 Jul 15. 103(1):101-5.

Type

Empty backbone

Use

S. aureus-e. coli shuttle vector

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

Plasmid

#194458

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-CD19 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-synORP1

Plasmid

#191680

Purpose

Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

rredox(roGFP2-synORP1

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR15.2-2H12.D11opt

Plasmid

#221185

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2.1

Expression

Bacterial

Promoter

Constitutive

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPRx-Sth1Cas9-L5

Plasmid

#140993

Purpose

Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

Depositor

Wilbert Bitter

Article

Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

Inserts

Sth1Cas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified; Integrative in mycobactreia

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEMG-GRIS

Plasmid

#225134

Purpose

For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDisplay-GRAB_UCN1.0

Plasmid

#208667

Purpose

Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Promoter

UBC

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A2_G_31AACTdel

Plasmid

#237352

Purpose

Expression plasmid for RSV A2 G

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV A2 G human codon optimized from Genbank Accesion KT992094.1

Expression

Mammalian

Mutation

31 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-SpG

Plasmid

#159978

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-SpG

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Pb-TRE3G-SpCas9-IRES-blast

Plasmid

#195506

Purpose

Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-HPH-C4

Plasmid

#160343

Purpose

High-copy expression vector for K. marxianus, hygromycin selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-His6dNLP

Plasmid

#105498

Purpose

Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

His6dNLP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PRKD1

Plasmid

#23500

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKD1 (PRKD1 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK340

Plasmid

#192639

Purpose

Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dSpG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dSpG has se…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-ASAP5-Kv2.1-WPRE

Plasmid

#225707

Purpose

Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Has Service

AAV1

Insert

ASAP5-Kv2.1

Use

AAV

Promoter

human synapsin

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-TDP-43

Plasmid

#235487

Purpose

Expression of human TDP-43-EGFP

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2962

Plasmid

#144438

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR2C2 (NR2C2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
BCL2alpha-MJK2243

Plasmid

#227690

Purpose

Expresses human BCL2alpha

Depositor

Curt Civin

Article

Goodis et al Eur J Med Chem. 2024 Dec 22;285:117190.

Insert

human BCL-2alpha (BCL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.2_mNeonGreen2

Plasmid

#113727

Purpose

Expresses the fluorescent protein mNeonGreen2 in a lentiviral backbone

Depositor

Vanessa LaPointe

Article

Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

Insert

mNeonGreen2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

Plasmid

#159905

Purpose

Mutagenesis of Slc32a1

Depositor

Larry Zweifel

Insert

Slc32a1 (Slc32a1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG261-His-greenFAST

Plasmid

#160349

Purpose

Expresses His-tagged greenFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-greenFAST

Expression

Bacterial

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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