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32,656 results

Showing: 4651 - 4700 of 32656 results

  1. pFP.E222

    Plasmid
    #138248
    Purpose
    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, aā€¦
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138248-plasmid-map-sequence-id-273451

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  7. pN565

    Plasmid
    #49990
    Purpose
    expresses an attenuated T7 RNAP under an isopropyl Ī²-d-1-thiogalactopyranoside (IPTG) inducible control
    Depositor
    Christopher Voigt
    Article
    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1
    Insert
    T7 RNAP*
    Use
    Synthetic Biology
    Tags
    umuD degradation tag
    Expression
    Bacterial
    Mutation
    R632S
    Promoter
    pTac-symmetric
    Available Since
    Jan. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    49990-plasmid-map-sequence-id-384231

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  9. pAWPSG-Po-nCas9-BE

    Plasmid
    #195740
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195740-plasmid-map-sequence-id-386428

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  12. pMBEC6

    Plasmid
    #187598
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187598-plasmid-map-sequence-id-367542

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  14. pET-MBP-Geo_st

    Plasmid
    #87700
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87700-plasmid-map-sequence-id-174647

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  15. pX2-Cas9

    Plasmid
    #85811
    Purpose
    Arabinose inducible Cas9 in a broad host range backbone.
    Depositor
    Ryan Gill
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    85811-plasmid-map-sequence-id-177180

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  16. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Article
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and contaā€¦
    Promoter
    IRES and tac
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pFEndoNA2

    Plasmid
    #174667
    Purpose
    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA2 fusion protein
    Tags
    Histag
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174667-plasmid-map-sequence-id-343065

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  22. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasĪ¦-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  24. pHUE-eGFP-Clu-tails

    Plasmid
    #215397
    Purpose
    Expression of a His6-Ubiquitin-Clu(228-238)-eGFP-Clu(204-227) fusion protein
    Depositor
    Franz-Ulrich Hartl
    Insert
    Clu(228-238)-eGFP-Clu(204-227) (CLU Human)
    Tags
    His6-Ubiquitin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215397-plasmid-map-sequence-id-430524

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  28. pBDC

    Plasmid
    #135188
    Purpose
    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    CmR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    None
    Promoter
    cat
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135188-plasmid-map-sequence-id-273773

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  34. FH

    Plasmid
    #38978
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FH (FH Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. ABL1-N-HiBiT kinase domain_v1

    Plasmid
    #207121
    Purpose
    Bacterially expressed HiBiT-ABL1 kinase domain (D233-S500) for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human Abelson Kinase Domain N-terminal HiBiT, N-terminal 10X His-tag (ABL1 Human)
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207121-plasmid-map-sequence-id-422565

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  49. T7 Lig-C-HiBiT

    Plasmid
    #215441
    Purpose
    Bacterially expressed T7 Lig-C-HiBit for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Escherichia phage T7 DNA Ligase, C-terminal HiBiT, C-terminal 10XHis.Thrombin tag
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 geneā€¦
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215441-plasmid-map-sequence-id-428723

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Showing: 4651 - 4700 of 32656 results