76,239 results
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pDEST40-2XHA-Src-K298M
Plasmid#140314PurposeExpresses HA-tagged human Src-K298M in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationK298MPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT G228A
Plasmid#84926Purposeluciferase reporter for mutant TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG228APromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCF806-shRen.713
Plasmid#186712PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).DepositorInsertshRen.713
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2
Plasmid#40978DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA
Plasmid#200641PurposeExpresses miniTurboID-FKBPF36V-2xHA in mammalian cells.DepositorInsertminiTurboID-6xGGSG linker-FKBPF36V-2xHA
UseTagsHA-tagExpressionMammalianMutationPromoterT7Available sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-miniAAVR-FLAG
Plasmid#166717PurposeVector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tagDepositorInsertminiAAVR-FLAG
UseLentiviralTagsFLAGExpressionMammalianMutationOnly expressing PKD1-3 of ectodomainPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
hCGNL1-Myc-HA
Plasmid#236515PurposeFor expression of hParacingulinL1 in mammalian cellsDepositorInsertCGNL1 (CGNL1 Human)
UseTagsMyc-HAExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pN7HELIX
Plasmid#190662PurposeExpresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
UseTagsExpressionMammalianMutationnAniI = K227M, F80K, L232KPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH051
Plasmid#171625Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-BsmBI-Destination
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
mNeon-Vamp2
Plasmid#186619PurposeEncodes Vamp 2 fused to mNeonGreenDepositorInsertVesicle Associated Membrane Protein 2 (VAMP2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLOX-CW-CRE
Plasmid#12238DepositorInsertCRE
UseCre/Lox and LentiviralTagsExpressionMammalianMutationCMV promoter is upstream of nls-CRE. LoxP in 3&#x…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
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CMV-d2eGFP-empty
Plasmid#26164DepositorInsert
UseTagsExpressionMammalianMutation…ccgcCTCGAG[sponge sites]GGGCCCgttt… became …ccgc…PromoterAvailable sinceAug. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pREP-8xARE-GFP-SV40-BFP
Plasmid#134910PurposeNRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFPDepositorInserts8xARE- minimal promoter - GFP
TagBFP
UseTagsExpressionMammalianMutationPromoter8xARE - minimal promoter and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry2(1-10)
Plasmid#82603PurposeLentiviral vector for constitutive expression of sfCherry2(1-10)DepositorInsertsfCherry2(1-10)
UseLentiviralTagsExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterSFFVAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-Fos
Plasmid#131593Purposedoxycycline-inducible expression of mouse c-Fos in mammalian cellsDepositorInsertFBJ osteosarcoma oncogene (Fos Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2164
Plasmid#142186PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGRHL2 (GRHL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBRed-d2-hygro
Plasmid#139196Purposenon-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puroDepositorInsertCBRed
UseLuciferase and RetroviralTagsd2 PEST domain and nuclear localization signal (N…ExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX2-Tango
Plasmid#66440PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertMRGPRX2 (MRGPRX2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EB3mCherry_cmv-LifeActGFP
Plasmid#133927PurposeLive dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeletonDepositorInsertEB3-mCherry and Lifeact-GFP (MAPRE3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR
Plasmid#204146PurposeLentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genesDepositorInsertsSARS-CoV-2 BA.1 variant spike
zsGreen-T2A-PuR
UseLentiviralTagsExpressionMammalianMutation21 amino acid cytoplasmic tail deletionPromoterCMV and TRE3GAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2509
Plasmid#144387PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEP300 (EP300 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-D
Plasmid#38133DepositorInsertMITF-D (MITF Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBJ FLAG-hPar1
Plasmid#53226Purposemammalian expression of human Par1 with N-terminal FLAG tagDepositorInsertPar1 (F2R Human)
UseTagsFLAG and bovine prolactin signal sequence (MDSKGS…ExpressionMammalianMutationDeletion of aa1-39 (native signal peptide)PromoterSV40Available sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
CSTB_pCSdest
Plasmid#53910DepositorInsertCSTB (CSTB Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceSept. 8, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRIP-SFFV-mtagBFP-2A STING
Plasmid#102586PurposeLentivector to express STING and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HA-Flag-natT1R2
Plasmid#113944Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839PromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 Wt (1-674aa)
Plasmid#75478Purposemammalian expression of GADD34DepositorInsertGADD34 (PPP1R15A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-IDH2-WT
Plasmid#87926PurposeExpresses IDH2 (wild-type) in mammalian cellsDepositorInsertisocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1092-luc2
Plasmid#227112PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
IDP432
Plasmid#87000Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertSNAP-Nlg1AB-1xλN(i)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP
Plasmid#169332PurposeNuclear expression of HaloTag9 in mammalian cellsDepositorInsertH2B-HaloTag9 (H2BC21 Human)
UseTagsHaloTagExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Flag-mCherry-Binder
Plasmid#179139PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
UseTagsFLAG and mCherryExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6_N7-sgRNA2
Plasmid#190664PurposeExpresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloningDepositorInsertN7 sgRNA2
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H2B-iRFP720
Plasmid#128961PurposeLentiviral plasmid for expression of far red iRFP720 nuclear markerDepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterhPGKAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40
Plasmid#124603PurposeCre-dependent soma-localized ChrimsonR for optogenetic stimulationDepositorHas ServiceAAV9InsertSoma-localized ChrimsonR
UseAAVTagsmRuby2ExpressionMammalianMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-FLAG-FAM134B
Plasmid#128260PurposeExpresses FAM134B in mammalian cellsDepositorInsertFAM134B (RETREG1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP
Plasmid#63215PurposeExpression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationPromoterCMV-EF1α hybrid (CEF)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sniper2L
Plasmid#193856PurposeExpresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSDDepositorInsertSniper2L
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationE1007LPromoterEFSAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PG13-mCherry
Plasmid#195291PurposemCherry reporter containing 13 copies of the p53-binding consensus sequenceDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV_Cas9_puro
Plasmid#65655PurposeRetroviral introduction of Cas9 into mammalian cell line.DepositorInsertCas9
UseCRISPR and RetroviralTags5 ' FLAG tagExpressionMammalianMutationPromoterMSSV_LTRAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab7 DN
Plasmid#12662DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsDsRed and HAExpressionMammalianMutationT22NPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pFLAG-HHARI
Plasmid#17450DepositorInsertHHARI (ARIH1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-K298M
Plasmid#140315PurposeExpresses Flag-tagged human Src-K298M in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationK298MPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161928PurposeDonor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine activation reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpEFAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only
