76,239 results

1. pLenti-hSynapsin-Cre-WPRE

   Plasmid

   #86641

   Purpose

   Expresses Cre in mammalian cells

   Depositor

   Fan Wang

   Article

   Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

   Insert

   Cre
   

   Use

   Cre/Lox, Lentiviral, Mouse Targeting, and Synthet…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-CMV-cGAS-HA

   Plasmid

   #130910

   Purpose

   Expresses human cGAS-HA; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   cGAS
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

   Plasmid

   #214102

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-R-Creon shRNA[Control]

   Plasmid

   #180775

   Purpose

   This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

   Depositor

   Eun Mi Hwang

   Article

   Kim et al J Neurosci Methods. 2021 Dec 21:109452.

   Type

   Empty backbone

   Use

   AAV, Cre/Lox, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-C1 Utr230-EGFP-3XNLS

   Plasmid

   #58466

   Purpose

   Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   Utr230 with 3X glycine linker (UTRN Human)
   3X NLS with 3X stop codons
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-HP1gamma

   Plasmid

   #17650

   Depositor

   Tom Misteli

   Article

   Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

   Insert

   HP1 gamma (CBX3 Human)
   

   Use

   Tags

   GFP and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFP-Bak

   Plasmid

   #32564

   Depositor

   Richard Youle

   Article

   Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

   Insert

   human Bak (BAK1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

   Plasmid

   #113163

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

   Plasmid

   #107270

   Purpose

   Expresses Cas9 upon induction with doxicycline.

   Depositor

   Pablo Menéndez

   Article

   Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

   Inserts

   hSpCas9
   rtTA
   bGH polyA-SV40polyA
   

   Use

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF2541

    Plasmid

    #142399

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX5 (SOX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-hNox4

    Plasmid

    #69352

    Purpose

    expresses human Nox4 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

    Insert

    hNOX4 (NOX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-BRD4ΔN-mCherry-sspB

    Plasmid

    #121968

    Purpose

    Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-461

    Promoter

    SFFV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-DN-hCUL4A-FLAG

    Plasmid

    #15821

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin4A (CUL4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N440

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1481 pcDNA3 flag HA 14-3-3 zeta

    Plasmid

    #9002

    Depositor

    William Sellers

    Insert

    14-3-3 zeta (YWHAZ Bovine)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    T226A

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRwB

    Plasmid

    #48734

    Purpose

    dsRed plasmid

    Depositor

    John Schiller

    Article

    Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

    Insert

    dsRed-Express
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP-p65

    Plasmid

    #111190

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

    Insert

    RELA (RELA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PIK3CA - Exon20 H1047R

    Plasmid

    #16639

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-PV-NLS-GFP

    Plasmid

    #17300

    Depositor

    Anton Bennett

    Article

    Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

    Insert

    PV-GFP-NLS (Pvalb Rat)
    

    Use

    Tags

    Myc and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4.0-TERT G250A

    Plasmid

    #84925

    Purpose

    luciferase reporter for mutant TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    G250A

    Promoter

    TERT

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-mPCSK4

    Plasmid

    #122675

    Purpose

    Expresses mouse PCSK4 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4) (Pcsk4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. wtTDP43tdTOMATOHA

    Plasmid

    #28205

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    HA tag and tdtomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLX209-neo-active WRN

    Plasmid

    #125788

    Purpose

    constitutive, ectopic expression of human active WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-active WRN (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GLASTp-DsRed2

    Plasmid

    #17706

    Depositor

    Nicholas Gaiano

    Article

    Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

    Insert

    GLASTp (SLC1A3 Human)
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 SARS-CoV-2 N

    Plasmid

    #158079

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 nucleocapsid (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(+) NAPstar3b

    Plasmid

    #232736

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEYFP-C1-Drp1

    Plasmid

    #45160

    Depositor

    Richard Youle

    Article

    Frank et al Dev Cell. 2001 Oct;1(4):515-25.

    Insert

    Drp1 (DNM1L Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 1479 pcDNA3 flag HA 14-3-3 gamma

    Plasmid

    #9000

    Depositor

    William Sellers

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

28. SP6-VEE-IRES-Puro

    Plasmid

    #58971

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Plasmid

    #154430

    Purpose

    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Depositor

    David Liu

    Article

    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

    Insert

    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    VRQR point mutations in SpCas9

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-mNaChBac-T2A-tdTomato

    Plasmid

    #60650

    Purpose

    Expresses mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    mNaChBac-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ISceI-GR-RFP

    Plasmid

    #17654

    Depositor

    Tom Misteli

    Article

    Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

    Insert

    I-SceI-GRLBD (SCEI, Nr3c1 Budding Yeast, Rat)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    C655G mutation in the glucocorticoid receptor (se…

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHCMV_VSBV_G

    Plasmid

    #207312

    Purpose

    Mammalian expression of Variegated Squirrel Bornavirus G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    VSBV_G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HBV 1.3-mer X-null replicon

    Plasmid

    #65461

    Purpose

    containing 1.3 units of the X-null HBV genome (subtype ayw)

    Depositor

    Wang-Shick Ryu

    Article

    Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

    Insert

    1.3 units of the X-null HBV genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

    Plasmid

    #141372

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. MSCV-Cre

    Plasmid

    #24064

    Purpose

    Retroviral coexpression of Cre and EGFP

    Depositor

    Dan Littman

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MESV 5' LTR

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP-SF2

    Plasmid

    #17990

    Depositor

    Tom Misteli

    Article

    Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains SF2 (aa1-210)

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. STING-V1

    Plasmid

    #124262

    Purpose

    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

    Depositor

    Eric Schirmer

    Insert

    TMEM173 (STING1 Human)
    

    Use

    Tags

    Venus1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Cherry-Fibrillarin-C1

    Plasmid

    #134539

    Purpose

    Expresses Cherry-Fibrillarin fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    Cherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GFP-C1-AKT-PH

    Plasmid

    #21218

    Depositor

    Tobias Meyer

    Article

    Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

    Insert

    AKT-PH
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of AKT. (Lab plasmid WC 0002).

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. CXCR2-DuET

    Plasmid

    #213219

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR2 (CXCR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PB-CT3G-CERP2-MG

    Plasmid

    #175503

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pPB-CAG-rtTA-IRES-Hygro

    Plasmid

    #102423

    Purpose

    Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

    Depositor

    Kian Peng Koh

    Article

    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

    Insert

    tetracyclin-transactivator (rtTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLenti CMVtight Blast DEST (w762-1)

    Plasmid

    #26434

    Depositor

    Eric Campeau

    Type

    Empty backbone

    Use

    Lentiviral; Tet-on advanced destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCMV4-3 HA/IkB-alpha

    Plasmid

    #21985

    Depositor

    Warner Greene

    Article

    Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

    Insert

    I-kappa B alpha (NFKBIA Human)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Fuw-dCas9-Dnmt3a

    Plasmid

    #84476

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid

    #224899

    Purpose

    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

    Depositor

    Thomas Norman

    Article

    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

    Inserts

    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA3.1 TaGu N-1F

    Plasmid

    #203762

    Purpose

    For expression of N-flag tagged TaGu R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA3-S33Y Beta-catenin

    Plasmid

    #19286

    Purpose

    Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S33Y (presumptive GSK3β phosphorylation site)

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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50. lentiCRISPRv2_AMBRA1#2

    Plasmid

    #174153

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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