75,142 results
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pCMV-T7-EGFP (BPK1098)
Plasmid#133962PurposeCMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcriptionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-mNeonGreen
Plasmid#188115PurposeTemplate plasmid to transcribe circular RNA encoding mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3
Plasmid#73021PurposeExpresses BE3 in mammalian cellsDepositorInsertBE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-NES
Plasmid#168301PurposeChemogenetic fluorescent H2O2 reporter targeted to the cytoplasmDepositorInsertHyPer7.2DAAO-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-GFP
Plasmid#180020PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-EGFP markerDepositorInsertPEmax-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL2- Empty control -SV40-Luc
Plasmid#26280PurposeAddgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF2304
Plasmid#142020PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCIITA (CIITA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbC-dsRED
Plasmid#84034PurposeContains Golden Gate cassette to express up to four gRNAs and dsREDDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag MKK6
Plasmid#13517DepositorInsertMKK6 (MAP2K6 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
Plasmid#119816Purpose3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cellsDepositorInsertsLuciferase
Puromycin
UseLentiviral and LuciferaseTagsEGFP and V5ExpressionMammalianMutationStop codon removed in the frame of the V5 frame v…PromoterCMV/TO and Human Phosphoglycerate kinaseAvailable sinceJan. 2, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
FUdeltaGW-rtTA
Plasmid#19780Purpose3rd generation lentiviral vectorDepositorInsertreverse tetracycline transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Bxb1
Plasmid#182142PurposeExpression of human codon-optimized Bxb1DepositorInserthuman codon optimized Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHP.AX
Plasmid#195218PurposeAAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)DepositorInsertSynthetic construct isolate AAV-PHP.AX VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-OVA
Plasmid#64599PurposeExpression of chicken ovalbumin as model antigen for study of MHC class I and class II presentationDepositorInsertOvalbumin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-HaloTag7-mGFP
Plasmid#184903PurposeStable expression of HaloTag7-mGFP in mammalian cells to measure autophagic fluxDepositorInsertmonomeric EGFP
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP670
Plasmid#136571PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertH2B-emiRFP670
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-RFP
Plasmid#127347PurposeExpresses RFP constitutively via the CAGGS promoter in mammalian cellsDepositorInsertRed Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterCAGGS PromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA
Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertSPgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS395_PhCMV-SB100
Plasmid#169633PurposeExpression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)DepositorInsertPCMV-driven SB100
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-hSyn-mCherry
Plasmid#87916PurposesgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)DepositorInsertmCherry
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterhSynAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Xist-PA
Plasmid#26760DepositorInsertXist (Xist Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry-a-tubulin
Plasmid#31930DepositorInsertPAmCherry1
UseTagsa-tubulinExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K33
Plasmid#17607PurposeMammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK33 only, other lysines mutated to arginines; P19…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 light chain
Plasmid#175358PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chainDepositorInsertanti-FLAG M2 light chain
UseTagsN-terminal secretion signal peptide (MEAPAQLLFLLL…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
UseTagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMito-LSSmOrange
Plasmid#37135DepositorInsertLSSmOrange
UseTagshuman cytochrome C oxidase subunit VIIIExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2
Plasmid#132775PurposePrime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3bDepositorInsertPE2
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF2-ABCG2-IRES-Neo
Plasmid#25983DepositorInsertATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
UseLentiviralTagsEF1-apha promoter and IRES-NeoExpressionMammalianMutationNonePromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR-HNRNPA1C-mCh-Cry2WT
Plasmid#101226PurposehnRNPA1(186-320) fused to mCh-Cry2WTDepositorInserthnRNPA1(186-320) (HNRNPA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pSBbi-Pur
Plasmid#60523PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Centrin2-N-10
Plasmid#55018PurposeLocalization: Centrosomes, Excitation: 587, Emission: 610DepositorInsertCentrin2 (CETN2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA-H2B-mScarlet3
Plasmid#224436PurposeConstruct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3DepositorInsertH2B-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMV & SP6Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX552
Plasmid#60958PurposepAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.DepositorInsertsU6_(SpaI)_sgRNA
Syn_EGFP-KASH
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMammalianMutationPromoterSynapsin and U6Available sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI WT
Plasmid#167289PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
gag/pol
Plasmid#14887DepositorInsertgag / pol
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC hTMEM115-3C-3xHA-P2A-eGFP
Plasmid#214996PurposeStably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.DepositorInsertTMEM115 (TMEM115 Human)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterUBCAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
gag-COM-pol
Plasmid#211373PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertMMLVgag-COM-pol
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29
Plasmid#22903DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-ER alpha
Plasmid#28230PurposeMammalian expression of Estrogen Receptor alpha fused to EGFPDepositorInsertEstrogen Receptor alpha (ESR1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENN239_CRISPRcharm_Kv1
Plasmid#220838PurposeCRISPRcharm Kv1 expression in mammalian cellsDepositorInsertCRISPRcharm Kv1
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 S HexaPro
Plasmid#154754PurposeMammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variantDepositorInsertSpike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTet-IRES-EGFP
Plasmid#64238PurposeLentiviral, doxycycline-inducible system to express transgene of interest and EGFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterpTetAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLDLR-Luc (aka: pES7)
Plasmid#14940DepositorInsertLDLR promoter (LDLR Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pmCherry Paxillin
Plasmid#50526DepositorInsertPXN paxillin (PXN Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI-hTFR1
Plasmid#218796PurposeRepCap for AAV productionDepositorInsertAAV Rep-Cap BI-hTFR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_sgRNA-ver2
Plasmid#126776PurposegRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNADepositorInsertsgRNA (for SpCas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-GFP
Plasmid#74165PurposeMammalian expression of EGFPDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only
