Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

169,512 results

Showing: 751 - 800 of 169512 results

  4. mTagRFP-T2-PCNA-19

    Plasmid
    #58043
    Purpose
    Localization: Nucleus, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    PCNA (PCNA Human)
    Tags
    mTagRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    58043-plasmid-map-sequence-id-175700

    Enlarge

  9. TFORF1914

    Plasmid
    #143553
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RUNX2 (RUNX2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143553-plasmid-map-sequence-id-400350

    Enlarge

  10. MSP2133

    Plasmid
    #72249
    Purpose
    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    Y450A, N497A, R661A, Q695A, and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72249-plasmid-map-sequence-id-131869

    Enlarge

  13. Membrane EGFP

    Plasmid
    #198056
    Purpose
    Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Insert
    LCK membrane EGFP (LCK A. victoria, Synthetic, Human)
    Use
    Sea urchin, mammal, zebrafish
    Tags
    EGFP
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    198056-plasmid-map-sequence-id-389155

    Enlarge

  24. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

    Enlarge

  27. pZac2.1 gfaABC1D-cyto-GCaMP8m

    Plasmid
    #186051
    Purpose
    AAV-mediated expression of GCaMP8m under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186051-plasmid-map-sequence-id-370165

    Enlarge

  28. qTAG-C-mNeon-Puro-TOMM20

    Plasmid
    #207790
    Purpose
    Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    207790-plasmid-map-sequence-id-415639

    Enlarge

  29. AV0-BWS-2.0-mC-G-CL3B

    Plasmid
    #124975
    Purpose
    evaluate autophagy
    Depositor
    Kazuhiro Oka
    Insert
    mCherry-GFP-LC3B
    Use
    AAV
    Promoter
    EF1-alpha
    Available Since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124975-plasmid-map-sequence-id-243955

    Enlarge

  33. TFORF1919

    Plasmid
    #141887
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RUNX1 (RUNX1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141887-plasmid-map-sequence-id-392593

    Enlarge

  45. mEmerald-Desmoglein1-N-18

    Plasmid
    #54064
    Purpose
    Localization: Desmosomes, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Desmoglein1 (DSG1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54064-plasmid-map-sequence-id-444156

    Enlarge

  49. mEmerald-Peroxisomes-2

    Plasmid
    #54228
    Purpose
    Localization: Peroxisomes, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Peroximal Targeting Signal 1 (PTS1)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 751 - 800 of 169512 results