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Showing: 851 - 900 of 174131 results

  10. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  17. pUC57-mitoLbNOX

    Plasmid
    #74448
    Purpose
    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Insert
    mitoLbNOX
    Tags
    Flag tag with linker and mitochondrial targeting …
    Mutation
    Human codon optimized
    Available Since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    74448-plasmid-map-sequence-id-203966

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  26. pET28-MBP-super TEV protease

    Plasmid
    #171782
    Purpose
    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    MBP-sTEV
    Tags
    Arg6 tag and Internal His6 tag
    Expression
    Bacterial
    Mutation
    No internal TEV cleavage site between the MBP and…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171782-plasmid-map-sequence-id-443047

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  29. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Article
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    Fok1 nuclease (homodimeric wildtype) and NLS
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Available Since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    52970-plasmid-map-sequence-id-82408

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  43. pWZL-Neo-Myr-Flag-DEST

    Plasmid
    #15300
    Purpose
    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF
    Depositor
    Jean Zhao
    Article
    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Flag and Myr
    Expression
    Mammalian
    Available Since
    July 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    15300-plasmid-map-sequence-id-190527

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  45. pAAV2/rh10

    Plasmid
    #112866
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap-rh10
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112866-plasmid-map-sequence-id-339914

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Showing: 851 - 900 of 174131 results