19,770 results

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LZRS-DNMT1

Plasmid

#24952

Depositor

Paul Khavari

Article

Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

Insert

DNMT1 (DNMT1 Human)

Use

Retroviral

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST-SorLA-v1

Plasmid

#154892

Purpose

Expresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

SORL1 (SORL1 Human)

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-HA-COPS5

Plasmid

#22541

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

COPS5 (COPS5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-human NPM1 wt

Plasmid

#160692

Purpose

Mammalian expression of FLAG-tagged NPM1

Depositor

Charles Sherr

Article

Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

Insert

NPM1 (NPM1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 M

Plasmid

#158078

Purpose

Expresses SARS-CoV-2 membrane protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 matrix (M )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

Plasmid

#69908

Purpose

Expresses a GFP circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1, GFP (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCB591

Plasmid

#141096

Purpose

E. coli-Lactobacilli shuttle vector for recombineering template cloning

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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DRH337: AAV-hsyn-CheRiff-eGFP

Plasmid

#51697

Purpose

AAV production of CheRiff channelrhodopsin actuator

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

CheRiff-eGFP

Use

AAV

Tags

eGFP

Promoter

hSynapsin

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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MELOPS

Plasmid

#80151

Purpose

Genetically encoded melanosome-localized pH sensor.

Depositor

Santiago Di Pietro

Article

Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

Insert

OCA2 (OCA2 Human)

Tags

mNectarine in first luminal loop

Expression

Mammalian

Mutation

nonpathologic polymorphism D257A, pore mutation V…

Promoter

CMV

Available Since

Sept. 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits
psicheck2 PTEN 3'UTR

Plasmid

#50936

Purpose

reporter for PTEN RNAi

Depositor

Pier Pandolfi

Article

Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

Insert

PTEN 3'UTR (PTEN Human)

Use

RNAi

Tags

Renilla luciferase

Expression

Mammalian

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-parkin C431S

Plasmid

#45877

Depositor

Edward Fon

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

C431S mutation (ubiquitin acceptor site)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pT7-gRNA

Plasmid

#46759

Purpose

gRNA cloning vector for in vitro transcription of target gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

gRNA core

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-TREX1(D18N)

Plasmid

#27220

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

D18N sequence 52-GAC changed to AAC, resulting i…

Available Since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pEB-C5

Plasmid

#28213

Purpose

5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

Insert

Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)

Expression

Mammalian

Available Since

June 20, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5M-ADAM10

Plasmid

#31717

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

ADAM10 (ADAM10 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pHM840

Plasmid

#20701

Depositor

Thomas Stamminger

Article

Sorg et al Biotechniques. 1999 May . 26(5):858-62.

Insert

GFP-NLS-lacZ

Expression

Mammalian

Available Since

April 29, 2009

Availability

Academic Institutions and Nonprofits only

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pSLQ1661-sgMUC4-E3(F+E)

Plasmid

#51025

Purpose

Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

Depositor

Bo Huang, Stanley Qi

Article

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

optimized sgRNA (MUC4 Human, Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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BE4

Plasmid

#100802

Purpose

Expresses BE4 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

BE

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HC-M

Plasmid

#173481

Purpose

An rrnB P1-based GFP ATP reporter in E. coli

Depositor

Rahul Sarpeshkar

Article

Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

Insert

GFP-mut2

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

rrnB P1

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGfaABC1D-nLac

Plasmid

#53131

Purpose

expression of lacZ driven by human GfaABC1D promoter

Depositor

Michael Brenner

Article

Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

Insert

Gfa2 (GFAP Human)

Use

Mouse Targeting

Tags

LacZ, NLS, and mP1

Expression

Mammalian

Mutation

contains ABC1D regions of Gfa2 promoter, -1757 to…

Promoter

human Gfa2

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

Plasmid

#122668

Purpose

C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Lentiviral

Tags

mCherry-SspB

Expression

Mammalian

Mutation

Contains only amino acids 186-230

Promoter

SFFV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDESTmycQKI

Plasmid

#19870

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

quaking homolog, KH domain RNA binding (QKI Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only
MigR1-AE9a

Plasmid

#12433

Depositor

Dong-Er Zhang

Article

Yan et al Nat Med. 2006 Aug . 12(8):945-9.

Insert

AML1-ETO9a fusion (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

AML-ETO protein with ETO exon 9a.

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti-smURFP-T2A-mCardinal

Plasmid

#80348

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
mCardinal

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti_MCP-LSD1_Hygro

Plasmid

#138457

Purpose

lenti vector encoding the MS2-LSD1 with a 2A Hygro resistance marker

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ecAT3.10

Plasmid

#107215

Purpose

Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

Depositor

Mathew Tantama

Article

Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

Insert

ecAT3.10

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGDestTol2LC-5sgRNA

Plasmid

#64243

Purpose

destination vector for five U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C3-Sec15

Plasmid

#53760

Purpose

pEGFP-C3 plasmid that expresses Sec15

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec15 (EXOC6 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
mTagBFP-Nucleus-7

Plasmid

#55265

Purpose

Localization: Nuclear Localization, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Insert

SV40 T-Antigen Nuclear Localization Signal (NLS)

Tags

mTagBFP-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

Plasmid

#46168

Purpose

Codon optimized Cas9_SV40 NLS with intron

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

codon optimized Cas9_SV40 NLS with intron

Use

CRISPR

Mutation

intron inserted at position 3113-3163, and SV40 N…

Promoter

eef-1A.1 (eft-3)

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-Raichu-RhoA-CR

Plasmid

#40258

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Raichu-RhoA-CR (RHOA Human, Synthetic)

Expression

Mammalian

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pmTurquoise2-Tubulin

Plasmid

#36202

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Alpha-tubulin (TUBA1B Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BE1

Plasmid

#73019

Purpose

Expresses BE1 with C-terminal NLS in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Kif5A-GFP-CIBN

Plasmid

#102252

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-GFP-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHN781

Plasmid

#178828

Purpose

Bacterial expression for human Saposin A

Depositor

Kazuhiro Abe

Insert

artificial gene encoding human Saposin A (AR Human)

Tags

His8, TEV

Expression

Bacterial

Mutation

Codon-usage was optimized for bacterial expressio…

Promoter

T7

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p52sheLL

Plasmid

#46950

Purpose

HPV52 L1 and L2 Bicistronic, too large to self-package

Depositor

John Schiller

Article

Kondo et al J Med Virol. 2008 May;80(5):841-6. doi:

Insert

HPV52L1+L2 (L1, L2 Human)

Expression

Mammalian

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2-hBIN1-V5

Plasmid

#27303

Depositor

Robin Shaw

Article

Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Insert

bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)

Tags

V5

Expression

Mammalian

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA4-Flag-Timeless

Plasmid

#22887

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

Insert

Timeless (TIMELESS Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted normal start codon, replaced with start c…

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-leu2d(empty)

Plasmid

#20120

Depositor

Jay Keasling

Article

Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP6-RNAP

Plasmid

#48143

Purpose

Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

rna polymerase SP6

Use

Synthetic Biology

Expression

Bacterial

Mutation

V314A (numbering based on NCBI Sequence ID: NP_85…

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIV_pTp66p51

Plasmid

#78233

Purpose

Expresses HIV reverse transcriptase

Depositor

Christopher Voigt

Article

Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

Inserts

p66
p51
LacI

Expression

Bacterial

Promoter

Const. Promoter (From DNA2.0 Vec) and pTac Minima…

Available Since

Aug. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-2XFL-wtSrc

Plasmid

#140295

Purpose

Expresses Flag-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Tags

2X Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1(+)-NEMOf

Plasmid

#189932

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOf

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmtb-t7-alpha-bungarotoxin

Plasmid

#69542

Purpose

For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

Depositor

Sean Megason

Article

Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

Insert

alpha-bungarotoxin

Use

Tol2, beta-actin promoter, sp6,t7 for zebrafish

Promoter

beta-actin, sp6, t7

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mCherry-MHC-IIA

Plasmid

#35687

Depositor

Venkaiah Betapudi

Article

Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

Insert

MHC IIA (MYH9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Clover-mRuby2

Plasmid

#49089

Purpose

Clover-mRuby2 tandem fusion for FRET calibration

Depositor

Kurt Beam

Insert

Clover-mRuby2 fusion

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
BcatMutS33/S37.T41/S45

Plasmid

#24204

Depositor

David Rimm

Insert

beta catenin (CTNNB1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S33A, S37A, T41A,S45A

Promoter

CMV

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CWB-yellow pre-eGRASP(p32)

Plasmid

#111580

Purpose

An AAV vector that expresses yellow pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p32)

Use

AAV

Promoter

CaMKIIa

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE-Medium-FT

Plasmid

#31914

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

PTight Tet-responsive PminCMVd

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge

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19,770 results