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162,717 results

Showing: 2151 - 2200 of 162717 results

  2. FURIN-bio-His

    Plasmid
    #51755
    Purpose
    Expresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    FURIN (FURIN Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    51755-plasmid-map-sequence-id-79339

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  5. hGli1 flag3x

    Plasmid
    #84922
    Purpose
    mammalian expression of Gli1
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli1 (GLI1 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84922-plasmid-map-sequence-id-422144

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  6. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

    Plasmid
    #89347
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences
    Depositor
    Ron Vale
    Article
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    Jurkat TCRalpha/beta alleles and CD3e and CD3z
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    89347-plasmid-map-sequence-id-176322

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  9. pSPObooster

    Plasmid
    #216160
    Purpose
    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains
    Depositor
    Grant Brown
    Article
    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978
    Inserts
    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    MKT1 gene containing an aspartic acid to glycine …
    Promoter
    endogenous promoter
    Available since
    May 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216160-plasmid-map-sequence-id-433867

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  10. pMBEC6

    Plasmid
    #187598
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187598-plasmid-map-sequence-id-367542

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  13. pCDF-Frb-v1

    Plasmid
    #201923
    Purpose
    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.
    Depositor
    Ryan Mehl, Richard Cooley
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (modified), T7 (modified, moderate), T7 (modif…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201923-plasmid-map-sequence-id-395412

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  24. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  26. hGli2 flag3x

    Plasmid
    #84920
    Purpose
    mammalian expression of Gli2
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli2 (GLI2 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84920-plasmid-map-sequence-id-203620

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  30. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Use
    Tags
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  31. AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610)

    Plasmid
    #191737
    Purpose
    AI plasmid ALIAS: AiP12610. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    191737-plasmid-map-sequence-id-376686

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  32. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Ryan Mehl, Richard Cooley
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  35. pYHY2

    Plasmid
    #216108
    Purpose
    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT0294 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    BfP1E6 promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216108-plasmid-map-sequence-id-434369

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  37. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid
    #217971
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    217971-plasmid-map-sequence-id-436106

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  39. pCW57.1-FOXM1b

    Plasmid
    #68811
    Purpose
    Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells
    Depositor
    Adam Karpf
    Article
    Barger et al Oncotarget. 2015 Jul 16.
    Insert
    FOXM1b (FOXM1 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68811-plasmid-map-sequence-id-419548

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  43. GATA3-pCDNA3.1

    Plasmid
    #115271
    Purpose
    Expresses human GATA3 in mammalian cells
    Depositor
    Arne S. Schaefer
    Insert
    GATA3 (GATA3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115271-plasmid-map-sequence-id-220672

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  49. pBMN-I-GFP

    Plasmid
    #1736
    Purpose
    This retroviral vector has an IRES-GFP downstream of the cloning sites
    Depositor
    Garry Nolan
    Insert
    IRES-GFP
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    1736-plasmid-map-sequence-id-418359

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Showing: 2151 - 2200 of 162717 results