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p15A_SpyCas9_CmR
Plasmid#119162PurposeExpresses S. pyogenes Cas9DepositorInsertCas9 from S. pyogenes (NEWENTRY )
UseCrisprTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
VTN-bio-His
Plasmid#53429PurposeExpresses full-length Vitronectin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertVTN (VTN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-gfp[tagless]
Plasmid#40164DepositorInsertPromotor probe
UseTagsExpressionMutationPromoterAvailable sinceDec. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos6_AmpR
Plasmid#119149PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_dCas7-11
Plasmid#197605PurposeExpresses inactive Cas7-11 in mammalian cellsDepositorInsertdCas7-11
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTK562 (pDS-GFP)
Plasmid#183129PurposeControl plasmid that expresses off-target dsRNADepositorInsertGFPmut3 (no RBS)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-H1.1
Plasmid#32894DepositorInsertHistone H1.1 (H1-1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BetaPix
Plasmid#129611PurposeRhoGEFDepositorInsertBeta-PIX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-pBAD
Plasmid#54666DepositorTypeEmpty backboneUseTags6xHis and mCeruleanExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF0429
Plasmid#143213PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPBX1 (PBX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2103
Plasmid#91061PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-eLACCO2.1-NGR
Plasmid#208023PurposeBiosensor for extracellular L-lactateDepositorInserteLACCO2.1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nuc-meCpG
Plasmid#186464PurposeExpresses mCherry tagged methyl-CpG recognition motif targeted to nucleus in mammalian cells.DepositorInsertdimeric MBD1 (MBD1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2987
Plasmid#144463PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRORA (RORA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ArcLight-ST
Plasmid#182318PurposeFluorescent voltage indicator with left-shifted voltage-dependencyDepositorInsertArcLight-ST
UseTagsExpressionMammalianMutationMutation in an amino acid Q217E of ArcLight-MTPromoterCAGAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PA-Dre
Plasmid#169706PurposePhotoactivatable Dre recombinase for AAV production and expression in mammalian cellsDepositorInsertDre246-nMag-T2A-pMag-Dre247
UseAAVTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 WT KSR1
Plasmid#25970DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB-NLS
Plasmid#228870PurposePE7 connected to MLH1-SB-NLS via 2A sequenceDepositorInsertPE7-2A-MLH1-SB-NLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER Long P2A
Plasmid#164107PurposeDetect the long Mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP Mt-ER Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 1-320
Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1103
Plasmid#142730PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGTF2IRD1 (GTF2IRD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-B
Plasmid#177785PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0612
Plasmid#143054PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRBPJ (RBPJ Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
RasV12 in pENTR1A (w99-1)
Plasmid#22252DepositorInsertHRAS G12V (HRAS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pLX304 3xFlag-eRF1_IRES-GFP
Plasmid#198393PurposeeRF1 expressionDepositorInserteRF1 (ETF1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_Csx29
Plasmid#197606PurposeExpresses Csx29 in mammalian cellsDepositorInsertCsx29
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N2cL
Plasmid#100812Purposeexpress rabies virus CVS N2c strain polymerase gene (N2cL)DepositorInsertrabies virus CVS N2c strain polymerase gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N2cN
Plasmid#100801Purposeexpress rabies virus CVS N2c strain nucleoprotein gene (N2cN)DepositorInsertrabies virus CVS N2c strain nucleoprotein gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N2cP
Plasmid#100808Purposeexpress rabies virus CVS N2c strain phosphoprotein gene (N2cP)DepositorInsertrabies virus CVS N2c strain phosphoprotein gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-4A-VC
Plasmid#110649Purposeexpresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR bindingDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsHA and VCExpressionMammalianMutationC379A, C385A, C421A and C427APromoterCMVAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tom20-CR
Plasmid#171461PurposeExpresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cellsDepositorInsertTom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDZ2FE_Pogz_1-1410
Plasmid#105674PurposeExpresses Flag-tagged full-length human POGZ proteinDepositorInsertPOGZ (POGZ Human)
UseTags2X Flag tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXP(V5_H2B_APEX2)
Plasmid#107597PurposeExpresses V5_H2B_APEX2 in mammallian cellsDepositorInsertH2B-APEX2
UseTagsH2B and V5ExpressionMammalianMutationPromoterCMV Dox inducibleAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
hC73S-pEGFP-N1
Plasmid#111536PurposeExpresses human KCNB1 C73S in mammalian cells for imagingDepositorInserthuman C73S-Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationC73SPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYX-C-mScarlet-Zeo-TSC22D2
Plasmid#222670Purposeendogenously tag TSC22D2 with C-terminal mScarletDepositorInsertTSC22D2 (TSC22D2 Human)
UseTagsmScarletExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ND1 Left -GSVG-UGI
Plasmid#187412Purposehuman mitochondrial DNA ND1 target TALEDepositorInsertDddA GSVG variant
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL
Plasmid#111394PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTags6xHis, Myc, T7, and XpressExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAK501
Plasmid#48107PurposelacZ promoter-probe plasmid with pVS1 origin of replication and chloramphenicol resistanceDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PER1_WT
Plasmid#82152PurposeGateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.DepositorInsertPER1 (PER1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-NLS-SNAP
Plasmid#174879PurposePiggyBac transposon vector for expression of NLS-SNAPDepositorInsertNLS-SNAP
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSExpressionMutationPromoterTRE, PGKAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMD.RVG.CVS24-N2c
Plasmid#19712DepositorInsertRabies virus CVS24-N2c glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pBMN mEGFP
Plasmid#188643PurposeThis is the Lazarou lab's standard retrovirus vector backbone for expression of mEGFP-tag fusions onto the N-terminus of cloned genesDepositorTypeEmpty backboneUseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget
Plasmid#214052PurposeExpresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.DepositorInsertTarget sequence
UseTarget rna plasmidTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNon-target
Plasmid#214053PurposeExpresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.DepositorInsertNon-target sequence
UseNon-target rna plasmidTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHochTypeIII
Plasmid#214039PurposeBacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6DepositorInsertCas10
UseCRISPRTagsExpressionMutationWTPromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only
