4,106 results

1. pcDNA-dCas9-VP64

   Plasmid

   #47107

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Flag, HA, SV40 NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR_PGK_LaG16_2_synNotch_Gal4VP64

   Plasmid

   #85421

   Purpose

   Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   LaG16_2 GFP nanobody synNotch Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42337

   Purpose

   Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C1(1-29)-TurboID-V5_pLX304

   Plasmid

   #107175

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Str-Golgin84_VSVG-SBP-EGFP

   Plasmid

   #65305

   Purpose

   synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPP441

   Plasmid

   #158801

   Purpose

   Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Use

   Tags

   2xSV40NLS-2xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-FLAG-CRBN-pGK-HYG

   Plasmid

   #107380

   Purpose

   Mammalian expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.2/v5-DEST hGlut4

   Plasmid

   #18087

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   Glut4 (SLC2A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV500 A-CREB

    Plasmid

    #33371

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    CREB (Creb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-GFAP-EGFP

    Plasmid

    #50473

    Purpose

    EGFP under the control of GFAP promoter

    Depositor

    Bryan Roth

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    GFAP

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBFP2-C1

    Plasmid

    #22880

    Depositor

    Dorus Gadella

    Article

    Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

    Insert

    SBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas9-sgAAVS1-1

    Plasmid

    #129726

    Purpose

    Expressing SpCas9 and sgAAVS1-1

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-1
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GalL

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLOVE empty vector

    Plasmid

    #15948

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PMXS-NDI1

    Plasmid

    #72876

    Purpose

    expresses NDI1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

    Insert

    NDI1 (NDI1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-myc3-bTrCP

    Plasmid

    #20718

    Depositor

    Yue Xiong

    Article

    Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

    Insert

    beta-transducin repeat containing (BTRC) (Btrc Mouse)
    

    Use

    Tags

    Myc3

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA 3 5' cMyc SMC1 wt

    Plasmid

    #32363

    Depositor

    Michael Kastan

    Article

    Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

    Insert

    SMC1A (SMC1A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. beta2-spectrin-HA

    Plasmid

    #31070

    Depositor

    Vann Bennett

    Insert

    beta2-spectrin (SPTBN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. T4 Lysozyme WT*

    Plasmid

    #18111

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No cysteines: C54T, C97A

    Promoter

    Available since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

    Plasmid

    #55639

    Purpose

    Flp-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

    Plasmid

    #126049

    Purpose

    For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

    Depositor

    François Bachand

    Article

    Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

    Insert

    TurboID
    

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CRE recombinase

    Plasmid

    #62730

    Purpose

    Expresses Cre recombinase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP-E-Syt1

    Plasmid

    #66830

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt1 (ESYT1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RelA cFlag pcDNA3

    Plasmid

    #20012

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Insert

    RelA (Rela Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLentiPGK Puro DEST p38KTRClover

    Plasmid

    #59152

    Purpose

    Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ampkar

    Plasmid

    #35097

    Purpose

    A fluorescent reporter of AMPK activity and cellular energy stress

    Depositor

    Lewis Cantley

    Article

    Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

    Insert

    ampkar
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    na

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGTR

    Plasmid

    #63143

    Purpose

    PCR template to construct polycistronic tRNA-gRNA

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    gRNA-tRNA fusion
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HA-EglN1-pcDNA3

    Plasmid

    #18963

    Depositor

    William Kaelin

    Article

    Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

    Insert

    Egg laying Nine - 1 (EGLN1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET mCherry LIC cloning vector (u-mCherry)

    Plasmid

    #29769

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.3_eGFP

    Plasmid

    #26822

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-eGFP-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. GFP::L4440

    Plasmid

    #11335

    Depositor

    Guy Caldwell

    Insert

    GFP
    

    Use

    RNAi

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV-beta-catenin deltaN90

    Plasmid

    #36985

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    beta-catenin deltaN90 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90 (Constitutively Acti…

    Promoter

    EF1a

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lentiGuide-Hygro-eGFP

    Plasmid

    #99375

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDest-566

    Plasmid

    #11517

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDuet TDP43 WT

    Plasmid

    #27462

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. 7TGP

    Plasmid

    #24305

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Raf-1 GST RBD 1-149

    Plasmid

    #13338

    Depositor

    Channing Der

    Article

    Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

    Insert

    Raf-1 (RAF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3-AKT-PH-GFP

    Plasmid

    #18836

    Depositor

    Craig Montell

    Article

    Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

    Insert

    AKT-PH domain (AKT1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains amino acids 1-148 (compared to GenBank r…

    Promoter

    CMV

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLX302

    Plasmid

    #25896

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. WWP-Luc (p21/WAF1 promoter)

    Plasmid

    #16451

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    WAF1 promoter (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid

    #140002

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHaloTag-EGFP

    Plasmid

    #86629

    Purpose

    Expresses HaloTag-eGFP tandem (cytosolic)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Flag-Bcl2

    Plasmid

    #18003

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS-DSCP

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLOVE-Nmyc

    Plasmid

    #15951

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

    Insert

    v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Promoter

    Available since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only