169,513 results
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pCAG-GCaMP6s
Plasmid#171156PurposeEncodes GCaMP6sDepositorInsertGCaMP6s
ExpressionMammalianAvailable SinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry
Plasmid#36084Purpose3rd generation lentiviral plasmid expressing mCherryDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUltra
Plasmid#24129Purpose3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interestDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianAvailable SinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-MAFB-IRF8-CEBPA
Plasmid#190720PurposeFor microglia differentiation from iPSCDepositorInsertMAFB-IRF8-CEBPA
ExpressionMammalianAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-BRD4-WT
Plasmid#90331Purposeexpresses Wild type BRD4DepositorAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionMutationDetailed in manuscriptPromoterT7 (inactivated)Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV_3xFLAG_NLS_ISCro4_NLS_T2A_puroR_SV40_polyA
Plasmid#251494PurposeExpression of a human codon optimised ISCro4 bridge recombinaseDepositorInsertISCro4 bridge recombinase
ExpressionMammalianMutationNAAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
Lenti‐Cas9‐2A‐Blast
Plasmid#73310PurposeLentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoterDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NSAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-neo
Plasmid#21916Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-HA-mScarlet3-tdP2A-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP
Plasmid#241139PurposeExpresses Anti-HA frankenbody-mScarlet3, anti-GCN4 scfv-sfGFP, and HaloTag-tdMCP to track mature and nascent HA and SunTag-tagged proteins and MS2 tagged mRNADepositorInsertsAnti-HA frankenbody
anti-GCN4 scfv
tdMCP
TagsHaloTag, mScarlet3, and sfGFPExpressionMammalianPromoterCMVAvailable SinceSept. 3, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
PLKO.1-Scrambled
Plasmid#136035PurposeScrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)DepositorInsertNone (Scrambled)
UseLentiviralExpressionMammalianAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-eGFP-Flag-SETX
Plasmid#218838PurposeMammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.DepositorAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn A53T
Plasmid#105727PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCh-Climp63
Plasmid#136293Purposeexpression of Climp63DepositorAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScaf
Plasmid#111401PurposePhagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18DepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2w/b
Plasmid#111568PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorHas ServiceAAV5InsertPlasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
UseAAVTagsmCherryAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.PI.EGFP.WPRE.bGH
Plasmid#105530PurposeAAV expression of EGFP from CMV promoterDepositorHas ServiceAAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVExpressionMammalianPromoterCMVAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPLICS LY-MT Long P2A
Plasmid#213613PurposeDetect the long-range Lysosome-Mitochondria contactDepositorInsertSPLICS LY-MT Long P2A
ExpressionMammalianAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJR101
Plasmid#187241PurposeLentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector for Perturb-seq with mU6 promoter
UseLentiviralAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCasKP-apr
Plasmid#117231PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagGFP2-Golgi-7
Plasmid#56415PurposeLocalization: Golgi Complex, Excitation: 483, Emission: 506DepositorInsertGolgi (B4GALT1 Human)
TagsmTagGFP2ExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable SinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…MutationP697S mutation in putative replicase. S239G and S…Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-lyso-pHluorin
Plasmid#70113PurposeExpression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidificationDepositorInsertlyso-pHluorin (Cd63 Aequorea victoria, Mouse)
TagsHA-Tag and pHluorinExpressionMammalianPromoterCMVAvailable SinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro
Plasmid#234070PurposeFor lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selectionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDB327
Plasmid#113671Purposeexpresses Ulp1_R3 in Escherichia coliDepositorInsertUlp1_R3
Tags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-R-iLACCO1
Plasmid#208025PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1
ExpressionMammalianAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xHA-TurboID-NLS_pCDNA3
Plasmid#107171Purposeexpresses 3xHA-tagged TurboID in the mammalian nucleusDepositorInsertTurboID (BirA mutant)
ExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-CMV
Plasmid#16405PurposeShuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralExpressionMammalianAvailable SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_dCas9_KRAB
Plasmid#126030PurposePiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusionDepositorInsertsdCas9 fusion with KRAB
Hygromycin resistance
UseCRISPR; PiggybacExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterEF1 alpha and TREAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDG02583
Plasmid#104129PurposeOptimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)DepositorInsertNEDD8-specific protease 1 (NEDP1)
Tags14x Histidine tag, Maltose binding protein, and S…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V
Plasmid#66789PurposeExpression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRalpha (Pdgfra Mouse)
TagsEGFPExpressionMammalianMutationD842V (constitutively active)PromoterEF1aAvailable SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
phU6-gRNA
Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman U6Available SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcVpx.myc
Plasmid#213388PurposeGenerate lentiviral vector which increases gene delivery into primary human myeloid cellsDepositorInsertSIVmac vpx (vpx Simian Immunodeficiency Virus)
UseLentiviralTagsmyc-6xHisExpressionMammalianPromoterCMVAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PDi-CRISPRn
Plasmid#73500PurposeDox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locusDepositorInsertsCas9
rtTA
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCAG and TREAvailable SinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-HP1alpha
Plasmid#17652DepositorAvailable SinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTRK892
Plasmid#71803PurposeExpression of mutated L. gasseri gusA from strong Ppgm promoterDepositorInsertgusA3
ExpressionBacterialMutationD573APromoterPpgmAvailable SinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)
Plasmid#129405Purposecircular Pepper RNADepositorInsertcircular F30-Pepper(TAR Variant-2)
ExpressionMammalianPromoterU6Available SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-deLACCO1-NGR
Plasmid#208024PurposeControl biosensor for extracellular L-lactateDepositorInsertdeLACCO1
ExpressionMammalianAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneTagsCub-PLVExpressionYeastAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-BEC enhancer-minBG-SYFP2
Plasmid#236722PurposeSYFP2-tagged Brain Endothelial Cell Enhancer mini Beta Goblin promoterDepositorTypeEmpty backboneUseAAVTagsSYFP2Promoterminimal beta globulinAvailable SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
PB-TA-ERN-ANKRD11
Plasmid#236615PurposeExpresses human ANKRD11 in mammalian cellsDepositorAvailable SinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tTA
Plasmid#104102PurposeExpresses tTA2 under the CAG promoter in mammalian cells.DepositorInserttTA2
ExpressionMammalianPromoterCAGAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-EGFP
Plasmid#122848PurposeN-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsEGFPExpressionMammalianPromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNX32-Dest
Plasmid#105083PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Puro
Plasmid#214402PurposeExpresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)DepositorUseLentiviralExpressionMammalianPromoterSFFVAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1-Cre-PGK-Puro
Plasmid#108543PurposeLentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoterDepositorInsertscre recombinase
Puromycin
PromoterEF1apha and PGKAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1
Plasmid#72266PurposeExpress gene of interest under EF-1 promoterDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-OVA
Plasmid#64599PurposeExpression of chicken ovalbumin as model antigen for study of MHC class I and class II presentationDepositorInsertOvalbumin
ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-mScarlet
Plasmid#227274PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only
