9,128 results

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pmCherry Paxillin

Plasmid

#50526

Depositor

Kenneth Yamada

Insert

PXN paxillin (PXN Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA6A-EGFR WT

Plasmid

#42665

Depositor

Mien-Chie Hung

Article

Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

Insert

EGFR (EGFR Human)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pKOR1

Plasmid

#133446

Purpose

Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

Depositor

Taeok Bae, Olaf Schneewind

Article

Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

Type

Empty backbone

Use

E.coli/s.aureus shuttle vector

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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FLHKII-pGFPN3

Plasmid

#21920

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKII cDNA sequence …

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ADAM10-HA

Plasmid

#65106

Purpose

Expression of HA tagged ADAM10 in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM10 (ADAM10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Cilantro 2

Plasmid

#74450

Purpose

Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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BASU RaPID plasmid

Plasmid

#107250

Purpose

Expresses the BASU RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

BASU

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDestTol2pACryGFP

Plasmid

#64022

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

GFP

Use

Gateway Cloning

Promoter

alpha-Acrystallin promoter

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-VAMP8

Plasmid

#45912

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 8 (VAMP8 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

Plasmid

#31481

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce target) EF BFP

Use

Lentiviral

Mutation

mCherry contains M9S and M16L to reduce backgroun…

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pMT-puro

Plasmid

#17923

Purpose

Insect expression vector

Depositor

David Sabatini

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Drosophila metallothionein gene promoter drives e…

Expression

Insect

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pINDUCER dCas9-TET1CD

Plasmid

#101921

Purpose

The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

dCas9-TET1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLM-fSV2A

Plasmid

#27512

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

OCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2

Use

Lentiviral

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-puro

Plasmid

#45561

Depositor

Michael McVoy

Article

Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

Insert

Puromycin N-acetyl transferase

Use

Bifunctional selection protein

Tags

EGFP

Expression

Mammalian

Promoter

Phcmv (Strong human cytomegalovirus immediate ear…

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-MHC-IIA

Plasmid

#35687

Depositor

Venkaiah Betapudi

Article

Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

Insert

MHC IIA (MYH9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-Ecadherin

Plasmid

#65286

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304-YAP1(S94A)

Plasmid

#59145

Purpose

YAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factors

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

S94A

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_PGK_LaG16_2_synNotch_Gal4VP64

Plasmid

#85421

Purpose

Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

LaG16_2 GFP nanobody synNotch Gal4VP64

Use

Lentiviral

Promoter

PGK

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-AAV-syn-jGCaMP8m-WPRE

Plasmid

#162375

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-BRD7

Plasmid

#65379

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD7 (BRD7 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
2XMyc-LRRK2-R1441C

Plasmid

#25363

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Arginine 1441 mutated to Cysteine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiPGK Puro DEST ERKKTRClover

Plasmid

#90227

Purpose

Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet_C1

Plasmid

#85042

Purpose

Mammalian expression of mScarlet

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p2xFLAGhYAP1-S127A

Plasmid

#17790

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein mutant S127A (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
shp53 pLKO.1 puro

Plasmid

#19119

Purpose

3rd gen lentiviral vector for knocking down p53 gene expression

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p53 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA(123)

Plasmid

#102613

Purpose

Expression of human RPA in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Inserts

RPA1 (RPA1 Human)
RPA2 (RPA2 Human)
RPA3 (RPA3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-mDlx-GFP-Fishell-1

Plasmid

#83900

Purpose

GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

eGFP

Use

AAV

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
spNW15

Plasmid

#173483

Purpose

Expresses spNW15 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW15

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1a-IRES-Neo

Plasmid

#28019

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Type

Empty backbone

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PGK GFP Puro (w509-5)

Plasmid

#19070

Purpose

3rd gen lentiviral eGFP expression vector, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-LC3 (human)

Plasmid

#24920

Purpose

Mammalian expression of LC3 fused to EGFP

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

LC3 (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
35S-eGFP-nosT

Plasmid

#80127

Purpose

Transient expression vector for eGFP in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Type

Empty backbone

Tags

eGFP

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
paavCAG-post-mGRASP-2A-dTomato

Plasmid

#34912

Purpose

post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Has Service

AAV5

Insert

postsynaptic mGRASP

Use

AAV

Promoter

CAG

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-JMJD3

Plasmid

#24167

Purpose

Mammalian expression of human JMJD3 with HA tag

Depositor

Kristian Helin

Article

Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

Insert

JMJD3 (KDM6B Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-myc-GLUT4-mCherry

Plasmid

#64049

Purpose

3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

GLUT4 (Slc2a4 Mouse)

Use

Lentiviral

Tags

c-Myc and mCherry

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV-AmphoEnv

Plasmid

#15799

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

4070A Amphotropic Murine leukemia virus envelope gene

Expression

Mammalian

Mutation

N/A

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
p2attPC

Plasmid

#51547

Purpose

DICE donor plasmid without neural transcription factors.

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

mCherry

Expression

Mammalian

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TagRFP-T-EEA1

Plasmid

#42635

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

EEA1 (EEA1 Human)

Tags

TagRFP-T

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-Neo-M2rtTA

Plasmid

#60843

Purpose

AAVS1 donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Article

DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

Inserts

M2rtTA
Neo

Use

Targeting donor

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HIV-1 LTR-gfp

Plasmid

#115809

Purpose

HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp and ngfr

Use

Lentiviral

Promoter

HIV1 LTR

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-GFP

Plasmid

#22612

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Alpha-Actinin-19

Plasmid

#54975

Purpose

Localization: Alpha Actinin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

actinin alpha 1 (ACTN1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Abl-His6-FLAG

Plasmid

#52684

Purpose

expression of Abl in mammalian cells

Depositor

Benjamin Turk

Article

Deng et al Chembiochem. 2012 Nov 26;13(17):2510-2.

Insert

ABL1 (Abl1 Mouse)

Tags

FLAG and His6

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSH-EFIRES-P-AtAFB2-mCherry

Plasmid

#129716

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk2-DN-HA

Plasmid

#1885

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDawn-sfGFP

Plasmid

#107741

Purpose

Expresses superfolder GFP under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

superfolder GFP

Expression

Bacterial

Promoter

pLambda

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTD103luxI_sfGFP

Plasmid

#48885

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

LuxI, sfGFP, and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdc2-DN-HA

Plasmid

#1889

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdc2 (CDK1 Human)

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag Jnk1a1(apf)

Plasmid

#13846

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(apf) (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif Thr(183)-Pro-T…

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL7.0: tgRFPt-SSPB WT

Plasmid

#60415

Purpose

Mammalian Expression of tgRFPt-Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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