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161,600 results

Showing: 3401 - 3450 of 161600 results

  2. pUBQ10-SNAP-GW

    Plasmid
    #209350
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    209350-plasmid-map-sequence-id-420465

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  3. pLCS110

    Plasmid
    #209331
    Purpose
    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal StayGold fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209331-plasmid-map-sequence-id-420445

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  4. pLNHA-C1-HseIF4G_O

    Plasmid
    #147087
    Purpose
    Mammalian Expression of HseIF4G
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4G (EIF4G1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation M432V compared to the seu…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147087-plasmid-map-sequence-id-289534

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  6. pLNHA-C1-HseIF4AI_K

    Plasmid
    #146731
    Purpose
    Mammalian Expression of HseIF4AI
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4AI (EIF4A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    a non silent mutation (A200V) and 3 silent mutati…
    Promoter
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    146731-plasmid-map-sequence-id-357210

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  7. pPOU5F1-donor

    Plasmid
    #176367
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    POU5F1 (POU5F1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176367-plasmid-map-sequence-id-347873

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  8. pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

    Plasmid
    #160628
    Purpose
    Module for the constitutive expression of the nptII, Cas9 and DsRed genes.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S, 35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160628-plasmid-map-sequence-id-321993

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  12. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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  13. pMSP1D1

    Plasmid
    #20061
    Purpose
    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477
    Insert
    MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    deletion mutant (1-11) of MSP1; His-tag on N-term…
    Promoter
    Available since
    March 16, 2009
    Availability
    Academic Institutions and Nonprofits only
    20061-plasmid-map-sequence-id-354445

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  15. lenti_dCas9-KRAB-MeCP2

    Plasmid
    #122205
    Purpose
    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.
    Depositor
    Andrea Califano
    Article
    Tan et al bioRxiv 2021.06.28.449297
    Insert
    dCas9-KRAB-MeCP2
    Use
    Lentiviral
    Tags
    KRAB-MeCP2
    Expression
    Mammalian
    Mutation
    Cas9 (D10A, H840A)
    Promoter
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122205-plasmid-map-sequence-id-377395

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  16. pET21a-Streptavidin-Alive

    Plasmid
    #20860
    Purpose
    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Alive
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Wt gene
    Promoter
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20860-plasmid-map-sequence-id-194024

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  19. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Promoter
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122650-plasmid-map-sequence-id-437776

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  21. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44719-plasmid-map-sequence-id-472945

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  22. M13-kmR

    Plasmid
    #206861
    Purpose
    M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)
    Depositor
    Irene Chen
    Article
    Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371
    Insert
    Kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    206861-plasmid-map-sequence-id-412778

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  23. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-466939

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  25. RNA-Protein Landing Pad

    Plasmid
    #226162
    Purpose
    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression
    Depositor
    Dustin Maly
    Article
    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.
    Inserts
    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    226162-plasmid-map-sequence-id-450826

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  28. EBFP2-N1

    Plasmid
    #54595
    Purpose
    Localization: N1 Cloning Vector, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54595-plasmid-map-sequence-id-364695

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  29. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  31. mCherry-Lysosomes-20

    Plasmid
    #55073
    Purpose
    Localization: Lysosome Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55073-plasmid-map-sequence-id-215601

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  32. AIO-GFP

    Plasmid
    #74119
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Article
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    74119-plasmid-map-sequence-id-454380

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  34. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40

    Plasmid
    #105545
    Purpose
    AAV expression of EGFP-Cre from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP-Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    HI
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105545-plasmid-map-sequence-id-202462

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  44. pSNAPf-ADRβ2 Control Plasmid

    Plasmid
    #101123
    Purpose
    Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells
    Depositor
    Ana Egana, New England Biolabs
    Insert
    Beta-2-Adrenergic Receptor (ADRB2 Human)
    Use
    Tags
    SNAP-tag (SNAPf)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101123-plasmid-map-sequence-id-194092

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  47. AcGFP1-C1

    Plasmid
    #54607
    Purpose
    Localization: C1 Cloning Vector, Excitation: 475, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    AcGFP1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54607-plasmid-map-sequence-id-162738

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Showing: 3401 - 3450 of 161600 results