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8,883 results

Showing: 3451 - 3500 of 8883 results

  3. pR_LG lenti-(empty)-del-CTS

    Plasmid
    #112895
    Purpose
    Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries
    Depositor
    Paul Blainey
    Article
    Feldman et al bioRxiv 262121
    Type
    Empty backbone
    Use
    Lentiviral
    Promoter
    none
    Available Since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112895-plasmid-map-sequence-id-217829

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  25. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79749-plasmid-map-sequence-id-443904

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  26. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90085-plasmid-map-sequence-id-182691

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  39. pCS-kI

    Plasmid
    #51553
    Purpose
    Mammalian phiC31 integrase expression vector. For use in applications where pseudosite integration is not desired.
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    phiC31 integrase
    Expression
    Mammalian
    Available Since
    March 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    51553-plasmid-map-sequence-id-261191

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  41. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  46. pAAV-Ef1a-DIO EYFP

    Plasmid
    #27056
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    EYFP
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    27056-plasmid-map-sequence-id-126562

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  49. AICSDP-15:ACTB-mEGFP

    Plasmid
    #87425
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87425-plasmid-map-sequence-id-174163

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Showing: 3451 - 3500 of 8883 results