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169,597 results

Showing: 3751 - 3800 of 169597 results

  4. pCMVR8.74

    Plasmid
    #22036
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Insert
    gag pol tat rev
    Expression
    Mammalian
    Available Since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22036-plasmid-map-sequence-id-339901

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  11. pGL6

    Plasmid
    #48746
    Purpose
    Expression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region (-1128 to +2129)
    Use
    Luciferase
    Available Since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. Mito-Dendra2

    Plasmid
    #238077
    Purpose
    Expresses photoactivable fluorescent reporter for visualizing mitochondrial network
    Depositor
    Partha Mondal
    Insert
    Dendra2
    Tags
    Mito
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    238077-plasmid-map-sequence-id-474211

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  18. WT-eGFP

    Plasmid
    #215168
    Purpose
    Encodes the enhanced green fluorescent protein
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    WT-eGFP
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215168-plasmid-map-sequence-id-430164

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  20. TFORF1885

    Plasmid
    #144114
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BRCA1 (BRCA1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144114-plasmid-map-sequence-id-396178

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  22. pHN924

    Plasmid
    #178885
    Purpose
    Expression of GST-His8-HRV3C
    Depositor
    Kazuhiro Abe
    Insert
    human rhinovirus 3C protease
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    178885-plasmid-map-sequence-id-354971

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  23. FUGW-H2B-mCherry

    Plasmid
    #132333
    Purpose
    Third generation lentiviral vector for hUbC-driven H2B-mCherry expression
    Depositor
    Bjoern Schwer
    Insert
    H2B-mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hUbC
    Available Since
    July 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    132333-plasmid-map-sequence-id-258771

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  26. TFORF2875

    Plasmid
    #143650
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ELF3 (ELF3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143650-plasmid-map-sequence-id-400466

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  37. SPLICSFAST-ER-PM-P2A

    Plasmid
    #214893
    Purpose
    Expresses equimolar Plasma Membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PM Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-ER-PM-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214893-plasmid-map-sequence-id-423479

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  45. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available Since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

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  49. cr3Vn

    Plasmid
    #184861
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, gam, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184861-plasmid-map-sequence-id-363183

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  50. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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Showing: 3751 - 3800 of 169597 results