161,202 results

1. TRE-UBC-HLA-E SCT-T2A-GFP

   Plasmid

   #205461

   Purpose

   lentiviral plasmid for expression of HLA-E SCT

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   HLA-E SCT (HLA-E Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myosin-IIA-GFP

   Plasmid

   #38297

   Depositor

   Matthew Krummel

   Article

   Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

   Insert

   Myosin II A (Myh9 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SPYtag-Histag-Avidin

   Plasmid

   #214836

   Purpose

   This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-Histag-Avidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEBB HA cIAP1

   Plasmid

   #38232

   Depositor

   Colin Duckett

   Article

   Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

   Insert

   cIAP1 (BIRC2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Use

   Tags

   81-bp polyadenylation

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmScarlet3-alphaTubulin_C1

   Plasmid

   #189768

   Purpose

   Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-alphaTubulin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NSK021 CMV-TO-p53R248Q (FLP-IN)

   Plasmid

   #191167

   Purpose

   Expression of human p53 mutant R248Q in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   p53(R248Q) (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R248Q

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. eSrtA(2A-9) pET29b

   Plasmid

   #75145

   Purpose

   reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

   Depositor

   David Liu

   Article

   Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   reprogrammed sortase A variant
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   S102C A104H E105D K138P K152I N160K K162H T164N K…

   Promoter

   T7

   Available since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

    Plasmid

    #213481

    Purpose

    AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    GFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-PTK2

    Plasmid

    #23902

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SPYtag-GFPnanobody

    Plasmid

    #214837

    Purpose

    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    SPYtag-GFPnanobody
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACYC-T7

    Plasmid

    #41187

    Depositor

    Dan Bolon

    Article

    Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

    Insert

    S. cerevisiae Hsp82
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. PG13-mCherry

    Plasmid

    #195291

    Purpose

    mCherry reporter containing 13 copies of the p53-binding consensus sequence

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EF1a-TET1-dCas9

    Plasmid

    #235593

    Purpose

    Tet1CD fused to N-terminus of dCas9

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
    

    Use

    CRISPR

    Tags

    3xNLS, TET1 CD, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE8.20-SpRY

    Plasmid

    #226859

    Purpose

    Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-SpRY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1 3xHA-KillerRed-OMP25

    Plasmid

    #174544

    Purpose

    localizes KillerRed to mitochondria

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    3xHA-KillerRed-OMP25
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R5 EMCV

    Plasmid

    #51733

    Purpose

    Dicistronic vector with EMCV IRES

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    EMCV IRES
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Non tagged Avidin

    Plasmid

    #214835

    Purpose

    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Non tagged Avidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-Bak

    Plasmid

    #32564

    Depositor

    Richard Youle

    Article

    Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

    Insert

    human Bak (BAK1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid

    #106965

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hsyn-GRAB_DA2m

    Plasmid

    #140553

    Purpose

    Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2m (medium affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p58sheLL

    Plasmid

    #37324

    Depositor

    John Schiller

    Article

    Kondo et al Virology. 2007 Feb 20;358(2):266-72. Epu

    Insert

    HPV58L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHRIG-Akt1

    Plasmid

    #53583

    Purpose

    lentiviral expression of constitutively active human Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    cAkt1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available since

    Oct. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Neurl1-Flag pcDNA3.1

    Plasmid

    #17282

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2007 Dec 12. ():.

    Insert

    Neuralized-like (Neurl1a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225241

    Purpose

    Mammalian expression of iPASnFR-H in the cytosol

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBabe puro IRES-EGFP

    Plasmid

    #14430

    Depositor

    L. Miguel Martins

    Has Service

    Cloning Grade DNA

    Insert

    IRES-EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AiP12315 - pAAV-AiE0464m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2315)

    Plasmid

    #223981

    Purpose

    AiE0464m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NLS-Laconic/pCMV-myc-nuc

    Plasmid

    #46307

    Purpose

    a nuclear-targeted, genetically-encoded sensor for lactate

    Depositor

    Luis Felipe Barros

    Article

    Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

    Insert

    NLS-Laconic
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ABE8e-NG

    Plasmid

    #226856

    Purpose

    Expresses ABE8e-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8e-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LT3GEPIR-WEE1shRNA1

    Plasmid

    #220571

    Purpose

    To inducibly knockdown WEE1 expression

    Depositor

    Sean Lee

    Insert

    WEE1 shRNA1 (WEE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LT3GEPIR-WEE1shRNA2

    Plasmid

    #220572

    Purpose

    To inducibly knockdown WEE1 expression

    Depositor

    Sean Lee

    Insert

    WEE1 shRNA2 (WEE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti_gRNA -hMLHdn-Puro

    Plasmid

    #191105

    Purpose

    Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    I31 silent mutation (ATA to ATC) to remove early …

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p31sheLL

    Plasmid

    #37322

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV31L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti TurboRFP BlastR

    Plasmid

    #102343

    Purpose

    Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

    Insert

    TurboRFP
    

    Use

    Lentiviral

    Tags

    N/A

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Cys-30nmSAH-SPYcatcher

    Plasmid

    #214839

    Purpose

    This plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-30nmSAH-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHR_SFFv_4D5-High-CAR_RHL002

    Plasmid

    #164825

    Purpose

    Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV10/3xflag-Clock

    Plasmid

    #47334

    Purpose

    full length clock in pCMV10 with 3x flag at the N-terminus

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    clock
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #210525

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCW57-RFP-P2A-MCS

    Plasmid

    #78933

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge