20,424 results

1. plenti-ATF4

   Plasmid

   #125238

   Purpose

   ATF4 expression vector

   Depositor

   Rozita Yarmand

   Article

   Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 4xnrUAS:GFP

   Plasmid

   #61373

   Purpose

   4xnrUAS driving GFP

   Depositor

   Marnie Halpern

   Article

   Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

   Insert

   4xnr UAS:GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-TRE-DIO-FLPo

   Plasmid

   #118027

   Purpose

   AAV vector for sparse and bright labeling in a cell-type specific manner

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

   Insert

   TRE-DIO-FLPo
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-EF1a-fDIO-Cre

   Plasmid

   #121675

   Purpose

   Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

   Depositor

   Alexander Nectow, Esteban Engel

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   NLS-CRE-HA
   

   Use

   AAV

   Tags

   3xHA and SV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBud-EGFP

   Plasmid

   #23027

   Depositor

   Andrea LeBlanc

   Article

   Jodoin et al PLoS One. 2009 . 4(8):e6647.

   Insert

   EGFP (eGFP )
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YEE-BE4max

   Plasmid

   #138157

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YEE)-nSpCas9-UGI-UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBb(RSF1010)1k-GFPuv

   Plasmid

   #106395

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p4054 HA-E6AP isoform II

   Plasmid

   #8658

   Depositor

   Peter Howley

   Article

   Kao et al J Virol 2000 Jul;74(14):6408-17.

   Insert

   E6-AP isoform II (UBE3A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCE-BiFC-VC155

   Plasmid

   #22020

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Nat Protoc. 2008 . 3(4):588-96.

   Insert

   VC155
   

   Use

   Tags

   HA

   Expression

   Worm

   Mutation

   Changed Alanine 206 to Lysine

   Promoter

   Available since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD-smURFP-RBS-HO-1

    Plasmid

    #80341

    Purpose

    Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    HO-1
    

    Use

    Tags

    6 Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSBbi-Bla

    Plasmid

    #60526

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Human Parkin (1-465)

    Plasmid

    #110758

    Purpose

    Bacterial expression of human Parkin

    Depositor

    David Komander

    Article

    Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

    Insert

    PRKN (PRKN Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 FLII12Pglu-700uDelta6

    Plasmid

    #17866

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLII12Pglu-700uDelta6
    

    Use

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    700uDelta6, ECFP insert in mglB

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1227 pcDNA3 Myr HA Akt2

    Plasmid

    #9016

    Depositor

    William Sellers

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABEpuro-CRAF

    Plasmid

    #51124

    Purpose

    mammalian expression of wt CRAF

    Depositor

    Matthew Meyerson

    Insert

    CRAF (RAF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFRT-DHX36iso1

    Plasmid

    #159585

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-H2B-GFP

    Plasmid

    #116869

    Purpose

    Nuclear localized GFP

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCGN-ATF6 (1-373)

    Plasmid

    #27173

    Purpose

    For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

    Depositor

    Ron Prywes

    Article

    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains only aa 1-373 (please see depositor comm…

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC016 - LwCas13a guide expression backbone with U6 promoter

    Plasmid

    #91906

    Purpose

    Backbone for cloning LwCas13a guides under U6 promoter

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Has Service

    Cloning Grade DNA

    Insert

    LwCas13a Guide site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pet28a-His6-Keap1

    Plasmid

    #62454

    Purpose

    His6 tagged Keap1 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSMT3-M

    Plasmid

    #26589

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    n/a
    

    Use

    Shuttle vector for mycobacteria

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pF CAG luc hygro

    Plasmid

    #67502

    Purpose

    Constitutive expression of firefly luciferase in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-EFS-MCP-HALOnls

    Plasmid

    #121937

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    MCP-HALOnls
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3.1(+)-NEMOf

    Plasmid

    #189932

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 4xmts-mNeonGreen

    Plasmid

    #98876

    Purpose

    In vivo visualization of mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    4xmts
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CHMP2A_GFP_N_term

    Plasmid

    #31805

    Depositor

    Daniel Gerlich

    Article

    Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Flag-HA-USP20

    Plasmid

    #22573

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP20 (USP20 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-Rab4B

    Plasmid

    #61801

    Purpose

    Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab4B (RAB4B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCgCas9_recT

    Plasmid

    #153337

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Has Service

    AAV1

    Insert

    mKate2-PDE4DCatL (PDE4D Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

    Plasmid

    #35152

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5, pTrc

    Available since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BASU RaPID plasmid

    Plasmid

    #107250

    Purpose

    Expresses the BASU RaPID labeling protein

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    BASU
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEB1-mVenus NB

    Plasmid

    #103986

    Purpose

    Plasmid encoding mVenus NB

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus NB
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1_nMagHigh1-EGFP-CAAX

    Plasmid

    #67300

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    nMagHigh1-EGFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CaV1.2

    Plasmid

    #26572

    Depositor

    Diane Lipscombe

    Article

    Helton et al J Neurosci. 2005 Nov 2. 25(44):10247-51.

    Insert

    Calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Pomc-pGL3

    Plasmid

    #17553

    Purpose

    Luciferase expression under control of Pomc promoter (–646 to +65).

    Depositor

    Domenico Accili

    Article

    Kitamura et al Nat Med. 2006 May . 12(5):534-40.

    Insert

    Pomc promoter (Pomc Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pomc

    Available since

    Oct. 17, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pU6-Sp-pegRNA-RNF2_+5GtoT

    Plasmid

    #135957

    Purpose

    S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    RNF2_5GtoT pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEF Synaptophysin-mRuby

    Plasmid

    #188980

    Purpose

    Synaptic buton marker

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Synaptophysin (Syp Synthetic, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-CAG-SomaGCaMP6f2 (AAV8)

    Viral Prep

    #158757-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Promoter

    CAG promoter

    Tags

    None

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

40. pHIV-dTomato

    Plasmid

    #21374

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUbC-nls-ha-stdMCP-stdGFP

    Plasmid

    #98916

    Purpose

    synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

    Depositor

    Robert Singer

    Article

    Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

    Insert

    stdMCP-stdGFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. sfGFP-pBAD

    Plasmid

    #54519

    Purpose

    Localization: Protein Expression Vector , Excitation: 485, Emission: 507

    Depositor

    Geoffrey Waldo, Michael Davidson

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    6xHis and SfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. GFP-PBRM1

    Plasmid

    #65387

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. HIVGKO

    Plasmid

    #112234

    Purpose

    HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

    Depositor

    Eric Verdin

    Article

    Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

    Insert

    csGFP-EF1a-mKO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDONR223-LATS1

    Plasmid

    #23665

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    LATS1 (LATS1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAAV-Ef1a-mCherry (AAV Retrograde)

    Viral Prep

    #114470-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

47. pGEx-4T-1-YTHDF1

    Plasmid

    #70087

    Purpose

    Encode YTHDF1 with N-terminal GST tag for E. coli expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pRK5-myc-Miro2

    Plasmid

    #47891

    Purpose

    Expresses myc tagged Miro2

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro2 (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGL4.22-PGK1-HRE::dLUC

    Plasmid

    #128095

    Purpose

    Hypoxia response element real-time luciferase reporter

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    

    Use

    Luciferase

    Tags

    drives expression of destabilized luciferase (2CP…

    Expression

    Mammalian

    Mutation

    Promoter

    inserted PGK1 hypoxia response elements (HRE) (x3)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. YFP-Parkin

    Plasmid

    #23955

    Purpose

    Mammalian expression of human Park2 fused to EYFP

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Park2 (PRKN Human)
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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