20,449 results

1. pLKO-RB1-shRNA19

   Plasmid

   #25640

   Purpose

   3rd generation lentiviral vector

   Depositor

   Todd Waldman

   Article

   Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

   Insert

   RB1-shRNA 19 (RB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-hA3A-eBE-Y130F

   Plasmid

   #113423

   Purpose

   Expresses hA3A-eBE-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MELOPS

   Plasmid

   #80151

   Purpose

   Genetically encoded melanosome-localized pH sensor.

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

   Insert

   OCA2 (OCA2 Human)
   

   Use

   Tags

   mNectarine in first luminal loop

   Expression

   Mammalian

   Mutation

   nonpathologic polymorphism D257A, pore mutation V…

   Promoter

   CMV

   Available since

   Sept. 20, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pcDNA Flag-RARalpha

   Plasmid

   #35555

   Depositor

   Jonathan Kurie, Harish Srinivas

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Use

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTriEx-mCherry-PA-Rac1-C450A

   Plasmid

   #22028

   Purpose

   Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
   

   Use

   Tags

   6xHis and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MIGR-RORgt

   Plasmid

   #24069

   Depositor

   Dan Littman

   Insert

   Rorgt (Rorc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAD43-daf-16 RNAi

   Plasmid

   #34833

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   daf-16 (daf-16 Nematode)
   

   Use

   RNAi

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 13, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pRevTRE MKL1

   Plasmid

   #19846

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pGWB555

   Plasmid

   #74885

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-AHPH-DM

    Plasmid

    #71368

    Purpose

    Location sensor for RhoA-GTP

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    C terminus of Anillin (ANLN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A740D and E758K

    Promoter

    CMV

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Rlucrarr3-Flash1

    Plasmid

    #74129

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCW57-RFP-P2A-MCS (Neo)

    Plasmid

    #89182

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGWB441

    Plasmid

    #74827

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-EGFR

    Plasmid

    #65225

    Purpose

    Expression of EGFR in mammalian cells

    Depositor

    Axel Ullrich

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. P2X4-pHluorin123

    Plasmid

    #52926

    Purpose

    express P2X4 protein with pHluorin at 123rd position

    Depositor

    Baljit Khakh

    Article

    Xu et al J Gen Physiol. 2014 Jul;144(1):81-104. d

    Insert

    mP2X4-pHluorin123
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-SENP1 (catalytic domain)

    Plasmid

    #16356

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP1 catalytic domain (SENP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 419 to 644 catalytic domain of human S…

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE9-His-p97(wt)

    Plasmid

    #14666

    Depositor

    Graham Warren

    Article

    Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

    Insert

    p97 (Vcp Mouse)
    

    Use

    Tags

    RGS6xHis

    Expression

    Bacterial

    Mutation

    See depositor comments below.

    Promoter

    Available since

    April 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD-LacI

    Plasmid

    #46394

    Purpose

    Overproduction of LacI

    Depositor

    Florian Hollfelder

    Article

    Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

    Insert

    LacI
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    silent T to C mutation at position 19

    Promoter

    arabinose promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEP4 E02S EN2L

    Plasmid

    #20922

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSAM_Bt

    Plasmid

    #112497

    Purpose

    Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

    Depositor

    Jeffrey Gordon, Andrew Goodman

    Article

    Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

    Inserts

    INSeq-adapted mariner transposon
    Himar1C9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

    Plasmid

    #132403

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG Cas9-2A-Citrine

    Plasmid

    #92358

    Purpose

    CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRP_ASC-LmCerulean

    Plasmid

    #41840

    Depositor

    Eicke Latz

    Article

    Stutz et al Methods Mol Biol. 2013;1040:91-101. doi:

    Insert

    apoptosis-associated speck-like protein containing CARD (PYCARD Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Stop Codon removed

    Promoter

    CMV

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAM2991

    Plasmid

    #40248

    Depositor

    Susan Golden

    Article

    Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

    Insert

    One Step Cloneing Vector for Overexpression
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMON-HsNMT1

    Plasmid

    #67475

    Purpose

    Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    HsNMT1 (NMT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

    Plasmid

    #141379

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. nls-NgAgo-GK

    Plasmid

    #78253

    Purpose

    Mammalian expression of NgAgo (Argonaute from N. gregoryi).

    Depositor

    Chunyu Han

    Article

    Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    NgAgo
    

    Use

    Tags

    a nuclear localization signal (NLS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid

    #139988

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFD152

    Plasmid

    #125546

    Purpose

    derivative of pFD116 with an optimized RBS for E. coli

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Insert

    optimized RBS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    anhydrotetracycline Ptet promoter

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLAM12

    Plasmid

    #26908

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Acetamidase Promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

31. HA-Elongin B-pcDNA3.1(+)-Zeo

    Plasmid

    #20000

    Depositor

    William Kaelin

    Article

    Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

    Insert

    transcription elongation factor B (ELOB Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAVS1-Neo-CAG-Flpe-ERT2

    Plasmid

    #68460

    Purpose

    AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    mammalian-codon-optimized Flpe recombinase fused with ERT2
    

    Use

    Synthetic Biology

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAGGS-SspB-mScarlet-I-MYPT169

    Plasmid

    #178522

    Purpose

    Expresses SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    SspB-mScarlet-I-MYPT169
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDEST17-UbE2T

    Plasmid

    #15808

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2T (UBE2T Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

35. pTRE-TIGHT-EGFP-donor fw copy

    Plasmid

    #22074

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    TETO-eGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mKO2-SLBP(18-126)

    Plasmid

    #83914

    Purpose

    Fluorescent probe for S/G2 cell cycle transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mKO2-SLBP(18-126) (SLBP Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mApple-C1

    Plasmid

    #54631

    Purpose

    Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pU6-sgCD71-2

    Plasmid

    #46918

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgCD71 -2 (TFRC Human)
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX458-DsRed2

    Plasmid

    #112219

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    DsRed2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DsRed2 contains a silent nucleotide substitution …

    Promoter

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GFP-ataxin-3

    Plasmid

    #89975

    Purpose

    Expresses GFP-tagged ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. VE-cad-eGFP Donor plasmid

    Plasmid

    #92309

    Purpose

    Knock eGFP into human VE-cad locus

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad Homologous Recombination Arms (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    about 30 a.a. truncated at end of protein

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEGFP-N1-delta30-TFEB

    Plasmid

    #44445

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFEB (TFEB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-30; H32D

    Promoter

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. HA-Vps34

    Plasmid

    #86749

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    human Vps34 (PIK3C3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

    Plasmid

    #55648

    Purpose

    Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre off/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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46. pTOM20-ddGFP-B

    Plasmid

    #40291

    Depositor

    Robert Campbell

    Article

    Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    ddGFP-B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCDNA3.3_CoV2_B.1.1.7

    Plasmid

    #170451

    Purpose

    Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Yuan et al Science. 2021 Aug 13;373(6556):818-823.

    Insert

    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLL7.0: Venus-iLID-CAAX (from KRas4B)

    Plasmid

    #60411

    Purpose

    Mammalian Expression of Venus-iLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. 3xFLAG-dCas9/pCMV-7.1

    Plasmid

    #47948

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A (catalyticall…

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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50. LAMP1-GFP

    Plasmid

    #16290

    Depositor

    Ron Vale

    Article

    Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

    Insert

    LAMP1/RE72002
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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