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166,671 results

Showing: 1601 - 1650 of 166671 results

  8. Gq-CASE

    Plasmid
    #168125
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F124/E125 within GNAQ
    Promoter
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168125-plasmid-map-sequence-id-332869

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  11. pMSP1D1

    Plasmid
    #20061
    Purpose
    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477
    Insert
    MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    deletion mutant (1-11) of MSP1; His-tag on N-term…
    Promoter
    Available Since
    March 16, 2009
    Availability
    Academic Institutions and Nonprofits only
    20061-plasmid-map-sequence-id-354445

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  14. Salsa6f

    Plasmid
    #140188
    Purpose
    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)
    Depositor
    Michael Cahalan
    Article
    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1
    Insert
    Salsa6f
    Use
    Tags
    tdTomato-v5-GCaMP6f
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Immediate Early
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140188-plasmid-map-sequence-id-316791

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  15. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV5)

    Viral Prep
    #55645-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available Since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  17. PB-CMV-MCS-Puro

    Plasmid
    #219794
    Purpose
    Mammalian expression plasmids using piggyBac Transposon System
    Depositor
    Keiichi Nakayama
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    219794-plasmid-map-sequence-id-441249

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  19. pCDH-EF1-mVenus-p27K−

    Plasmid
    #176651
    Purpose
    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
    Depositor
    Mohamed Bentires-Alj
    Article
    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:
    Insert
    mVenus-p27K- (Cdkn1b Mouse)
    Use
    Lentiviral
    Tags
    mVenus
    Expression
    Mutation
    Mutant K- (lacks affinity to Cdk)
    Promoter
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    176651-plasmid-map-sequence-id-354255

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  25. pLenti-PalmGRET

    Plasmid
    #158221
    Purpose
    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.
    Depositor
    Charles P. Lai
    Article
    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014
    Insert
    PalmGFP-Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    158221-plasmid-map-sequence-id-309022

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  29. pAAV-CAG-dLight1.3b (AAV5)

    Viral Prep
    #125560-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci
    Promoter
    CAG
    Tags
    None
    Available Since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  30. AAV-CAG-jGCaMP8f-WPRE (AAV Retrograde)

    Viral Prep
    #179254-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CAG
    Tags
    None
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  31. pGWB-nLUC

    Plasmid
    #174050
    Purpose
    Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay
    Depositor
    Alberto Macho
    Article
    Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    N-terminal luciferase
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174050-plasmid-map-sequence-id-342879

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  40. pHIV-Luciferase

    Plasmid
    #21375
    Purpose
    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    21375-plasmid-map-sequence-id-357945

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  50. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pTRE3G-bidirectional
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

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Showing: 1601 - 1650 of 166671 results