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169,450 results

Showing: 1651 - 1700 of 169450 results

  6. pBBR1-P2-SpCas9

    Plasmid
    #232354
    Purpose
    The plasmid pBBR1-P2-SpCas9 employs pBBR1MCS-2 as the vector, with Cas9 protein derived from S. pyogenes and the stationary phase promoter P2 sourced from S. marcescens HBQA7.
    Depositor
    Yujie Cai
    Article
    Gou et al Biotechnol Bioeng. 2025 May;122(5):1233-
    Inserts
    Cas9
    sacB
    Use
    CRISPR
    Promoter
    promoter P2 from S. marcescens HBQA7
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    232354-plasmid-map-sequence-id-485173

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  7. pEdit

    Plasmid
    #232355
    Purpose
    The pEdit series plasmids are derived from the pTarget series plasmids by inserting homologous recombination arms targeting the gene of interest, which enables gene knockout or gene insertion at the t
    Depositor
    Yujie Cai
    Article
    Gou et al Biotechnol Bioeng. 2025 May;122(5):1233-
    Insert
    sgRNA
    Use
    CRISPR
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    232355-plasmid-map-sequence-id-485174

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  12. pMRX-IP-EGFP-PA-BAX(mito, G108V)

    Plasmid
    #233343
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-OMP25 in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233343-plasmid-map-sequence-id-469412

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  16. pcDNA3.1(+)GαsS-246-Nluc

    Plasmid
    #207157
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAS (GNAS Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(246)N
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207157-plasmid-map-sequence-id-410282

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  18. pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV1)

    Viral Prep
    #83898-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    mCherry
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  22. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid
    #196720
    Purpose
    Tet-inducible expression of Cas9-T2A-TagBFP
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196720-plasmid-map-sequence-id-389756

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  23. pAAV-CKIIa-stGtACR2-FusionRed (AAV1)

    Viral Prep
    #105669-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    CaMKII
    Tags
    FusionRed
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. pAAV-hSyn-hChR2(H134R)-mCherry (AAV9)

    Viral Prep
    #26976-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-hChR2(H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-mCherry plasmid DNA. Synapsin-driven, humanized channelrhodopsin H134R mutant, fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  32. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a / U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139462-plasmid-map-sequence-id-308474

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  41. pZac2.1-GfaABC1D-lck-jGCaMP8f (AAV9)

    Viral Prep
    #176759-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8f (#176759). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8f plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    GfaABC1D
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  42. pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV9)

    Viral Prep
    #83896-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato (physically separate, not a fusion protein)
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  44. pAAV-DIO-ATLASsnFlp

    Plasmid
    #232352
    Purpose
    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.
    Depositor
    Don Arnold
    Article
    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d
    Has Service
    AAV8
    Insert
    ATLASsnFlp
    Use
    AAV
    Tags
    ALFA-Tag
    Promoter
    hSyn
    Available Since
    May 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    232352-plasmid-map-sequence-id-471581

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  45. pAAV-Syn-ChR2(H134R)-GFP (AAV Retrograde)

    Viral Prep
    #58880-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Syn-ChR2(H134R)-GFP (#58880). In addition to the viral particles, you will also receive purified pAAV-Syn-ChR2(H134R)-GFP plasmid DNA. Humanized channelrhodopsin H134R mutant fused to GFP, under the control of the Synapsin promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.
    Promoter
    Syn
    Tags
    GFP
    Available Since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only

  46. Lamp1-RFP

    Plasmid
    #1817
    Purpose
    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Article
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Available Since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    1817-plasmid-map-sequence-id-350820

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  48. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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Showing: 1651 - 1700 of 169450 results