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166,751 results

Showing: 1951 - 2000 of 166751 results

  3. pAAV.Syn.GCaMP6s.WPRE.SV40 (AAV1)

    Viral Prep
    #100843-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.GCaMP6s.WPRE.SV40 (#100843). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Tags
    None
    Available Since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Article
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

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  11. pSIJ8

    Plasmid
    #68122
    Purpose
    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression
    Depositor
    Alex Nielsen
    Article
    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103
    Inserts
    flp recombinase
    rhaS
    rhaR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rhamnose
    Available Since
    Dec. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68122-plasmid-map-sequence-id-243302

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  12. pGFP Cortactin

    Plasmid
    #50728
    Purpose
    mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    Cortactin (CTTN Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50728-plasmid-map-sequence-id-339652

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  21. pMCB320

    Plasmid
    #89359
    Purpose
    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites
    Depositor
    Michael Bassik
    Article
    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103
    Insert
    puromycin resistance, mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89359-plasmid-map-sequence-id-176457

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  23. enIscB

    Plasmid
    #205410
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    205410-plasmid-map-sequence-id-421947

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  28. MoClo Yeast Toolkit

    Plasmid Kit
    #1000000061
    Purpose
    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes
    Depositor
    John Dueber
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. PB-TO-NFIA.1-SOX9

    Plasmid
    #182310
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182310-plasmid-map-sequence-id-488651

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  31. pCW57-MCS1-2A-MCS2

    Plasmid
    #71782
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71782-plasmid-map-sequence-id-222919

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  35. ColorfulCell

    Plasmid
    #62449
    Purpose
    MXS_chaining vector with 6 cassettes
    Depositor
    Pierre Neveu
    Article
    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do
    Inserts
    CMV::GTS-Citrine-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::NLS-TagBFPx3-bGHpA
    CMV::MTS-AzamiGreen-bGHpA
    CMV::iRFP670-SKL-bGHpA
    CMV::ETS-mCherry-KDEL-bGHpA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    62449-plasmid-map-sequence-id-209509

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  37. Dual-sgRNA_hU6-mU6

    Plasmid
    #154194
    Purpose
    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154194-plasmid-map-sequence-id-308648

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  38. pAAV-hSyn-hM4D(Gi)-mCherry (AAV2)

    Viral Prep
    #50475-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  42. pEcCas

    Plasmid
    #73227
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
    Depositor
    Sheng Yang
    Article
    Li et al Acta Biochim Biophys Sin (Shanghai). 202
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR; Red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native promoter
    Available Since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73227-plasmid-map-sequence-id-419024

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  45. pAAV2/6

    Plasmid
    #240485
    Purpose
    AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein
    Depositor
    Addgene Research Program, Meghan Rego
    Insert
    AAV2 Rep and AAV6 Cap
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    240485-plasmid-map-sequence-id-474484

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  49. pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (AAV8)

    Viral Prep
    #137148-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (#137148). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of Arch3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Cre and Flp-dependent)
    Available Since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only

  50. pAAV-hSyn-GRAB_NE1m (AAV9)

    Viral Prep
    #123308-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NE1m (#123308). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NE1m plasmid DNA. Synapsin-driven expression of GRAB-NE1m norepinephrine sensor in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Promoter
    Syn
    Tags
    None
    Available Since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1951 - 2000 of 166751 results