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161,670 results

Showing: 1951 - 2000 of 161670 results

  3. pEcCas

    Plasmid
    #73227
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
    Depositor
    Sheng Yang
    Article
    Li et al Acta Biochim Biophys Sin (Shanghai). 202
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR; Red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native promoter
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73227-plasmid-map-sequence-id-419024

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  4. pLVX-puro-cOVA-IRES-BFP

    Plasmid
    #135074
    Purpose
    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.
    Depositor
    Maria Castro
    Insert
    cOVA-IRES-BFP (OVAL Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    135074-plasmid-map-sequence-id-266791

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  10. p-dCas9-LSD1-Hygro

    Plasmid
    #104406
    Purpose
    transient expression of dCas9-LSD1 fusion protein
    Depositor
    Stephan Beck
    Insert
    LSD1 (KDM1A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104406-plasmid-map-sequence-id-204465

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  12. pAAV-hSyn-EGFP (AAV Retrograde)

    Viral Prep
    #50465-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. Synapsin-driven EGFP-expression control. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  17. pCMVR8.74

    Plasmid
    #22036
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Insert
    gag pol tat rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22036-plasmid-map-sequence-id-339901

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  19. pAAV.hSyn.iGluSnFr.WPRE.SV40

    Plasmid
    #98929
    Purpose
    AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    iGluSnFr
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    98929-plasmid-map-sequence-id-190089

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  21. pAAV-hSyn-hM4D(Gi)-mCherry (AAV2)

    Viral Prep
    #50475-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV Retrograde)

    Viral Prep
    #20298-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Oct. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  28. AAV-GfaABC1D-Cre-4x6T (AAV5)

    Viral Prep
    #196410-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV-GfaABC1D-Cre-4x6T (#196410). In addition to the viral particles, you will also receive purified AAV-GfaABC1D-Cre-4x6T plasmid DNA. GfaABC1D driven expression of Cre recombinase in astrocytes. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Promoter
    CAG and GfaABC1D
    Tags
    None
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  30. pAAV-hSyn-DIO-EGFP (AAV Retrograde)

    Viral Prep
    #50457-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  32. Lenti-LucOS

    Plasmid
    #22777
    Depositor
    Tyler Jacks
    Insert
    OVA-SIY
    Use
    Cre/Lox and Lentiviral
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22777-plasmid-map-sequence-id-419746

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  36. AAV-CamKIIa-jGCaMP8m-WPRE (AAV1)

    Viral Prep
    #176751-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CamKIIalpha
    Tags
    None
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  38. pGP-AAV-syn-iGABASnFR2-WPRE (AAV1)

    Viral Prep
    #218874-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-iGABASnFR2-WPRE (#218874). In addition to the viral particles, you will also receive purified pGP-AAV-syn-iGABASnFR2-WPRE plasmid DNA. Syn-driven expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Kolb et al bioRxiv 2025.03.25.644953
    Promoter
    Syn
    Tags
    None
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  40. pMAL-AGR2

    Plasmid
    #18097
    Depositor
    Ronny Drapkin
    Insert
    AGR2 (AGR2 Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only

  41. pAAV-hSyn-hM4D(Gi)-mCherry (AAV1)

    Viral Prep
    #50475-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  45. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128096-plasmid-map-sequence-id-249669

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Showing: 1951 - 2000 of 161670 results