177,993 results
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Plasmid#149333PurposeBacterial expression of SENP_EuB proteaseDepositorInsertSENP_EuB protease
TagsHis14 and Tev protease cleavage siteExpressionBacterialAvailable SinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sybody Generation Toolbox
Plasmid Kit#1000000160PurposePlasmids to generate synthetic nanobodies against soluble proteins, membrane proteins, and protein complexes.DepositorApplicationGene Expression and LabelingVector TypeBacterial ExpressionAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-G3
Plasmid#135521PurposeEngineered form of fibroblast growth factor 2 with improved thermostabilityDepositorInsertfibroblast growth factor 2 (FGF2 Human)
Tags6x His tag and Agg Thrombin TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV SYN flex PSAM4 GlyR IRES EGFP (AAV5)
Viral Prep#119741-AAV5PurposeReady-to-use AAV5 particles produced from AAV SYN flex PSAM4 GlyR IRES EGFP (#119741). In addition to the viral particles, you will also receive purified AAV SYN flex PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven, Cre-dependent expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available SinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)
Plasmid#101139PurposeLuciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
Plasmid#61628PurposeExpresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoterDepositorInsertsntdTomato
n2xEGFP
ExpressionPlantMutation2 copies of EGFP with N-terminal SV40 NLSPromoterAuxin-dependent DR5 promoter containing 9 reverse…Available SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-Cetn1
Plasmid#50717PurposeCetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.DepositorAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE PGK-GFP-IRES-LUC-W
Plasmid#46793Purpose2nd generation lentiviral vectorDepositorInsertsUseLentiviralAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL01009::AtU6p
Plasmid#46968PurposeEncodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.DepositorInsertAtU6p
UseCRISPRExpressionPlantAvailable SinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pN1 Lck-GCaMP5G
Plasmid#34924PurposeExpresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membraneDepositorInsertLck-GCaMP5G
ExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterCMVAvailable SinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Hygro empty (w214-1)
Plasmid#17484Purpose3rd gen lentiviral empty control vector, CMV/TO promoter, HygroDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BeGFP
Plasmid#21210DepositorAvailable SinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-cMYC
Plasmid#20324DepositorAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycDICER
Plasmid#19873DepositorAvailable SinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBabe-c-myc-zeo
Plasmid#17758DepositorAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
JD633
Plasmid#160393PurposeCRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloningDepositorInsertsZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA
UseBinary vectorAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC G3BP1-GFP-GFP
Plasmid#119950PurposeLentiviral vector expressing G3BP1-GFP fusion proteinDepositorAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-hygro-STING
Plasmid#102598PurposeExpress STING for IFN-beta luciferase reporter assay.DepositorAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mScarlet-I
Plasmid#98827PurposeIn vivo visualization of lysosomes (can be used for colocalization studies)DepositorInsertLamp1
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-3xFLAG-V5
Plasmid#87071PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneUseLentiviralTags3xFLAG-V5ExpressionMammalianAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_AAVS1_T2
Plasmid#79888PurposeExpresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.DepositorTypeEmpty backboneUseCRISPRTagsUntagged eSpCas9(1.1)ExpressionMammalianPromoterCBhAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1- dCas9-2xNLS-EGFP
Plasmid#74710PurposeExpresses dCas9-2xNLS-EGFP in mammalian cellsDepositorInsertdCas9-2xNLS-EGFP
UseCRISPRTagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GMutationS34C mutation in NefAvailable SinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
murine TCR OTII-2A.pMIG II
Plasmid#52112Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorUseRetroviralTagsP2A (from porcine teschovirus-1)ExpressionMammalianAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mNeptune2.5
Plasmid#51310PurposeExpresses far-red fluorescent protein mNeptune2.5 in mammalian cellsDepositorInsertmNeptune2.5
ExpressionMammalianPromoterCMVAvailable SinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight Puro DEST (w768-1)
Plasmid#26430DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorExpressionMammalianAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Hu-L-Myc
Plasmid#26022DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
VCP(DK0)-EGFP
Plasmid#23974DepositorInsertValosin containing protein (VCP Human)
Tags6xHis, EGFP, and HAExpressionMammalianMutationE305QE578QAvailable SinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Ataxin3Q84
Plasmid#22123DepositorAvailable SinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
12.4kbVillin-ΔATG
Plasmid#19358DepositorInsert12.4kb Villin promoter
ExpressionBacterialMutationNo ATG - transgenes cloned downstream of the vill…Available SinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lac-I-SceI-Tet
Plasmid#17655DepositorInsertlac I-SceI tet
ExpressionBacterialAvailable SinceMay 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pSV Sport SREBP-1c
Plasmid#8883DepositorAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8m-WPRE (AAV Retrograde)
Viral Prep#162375-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Venus-Akaluc (neo)
Plasmid#124701PurposeAka-Luciferase reporterDepositorInsertVenus-Akaluciferase
UseLentiviralPromoterhPGKAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (AAV9)
Viral Prep#124603-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (#124603). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 plasmid DNA. EF1a-driven, Cre-dependent expression of ChrimsonR fused to mRuby2 fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorTagsmRuby2 (Cre-dependent)Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-EGFP
Plasmid#122848PurposeN-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseGateway Cloning and LentiviralTagsEGFPExpressionMammalianPromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
px330-mcherry
Plasmid#98750PurposeCas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
DLX2-hygro
Plasmid#97330PurposeGenerating lentiviruses to express Dlx2 and hygromycin resistanceDepositorAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMA4008
Plasmid#70109PurposemtDNA destruction/production of rho-0 cellsDepositorInsertsEGFP
Herpes simplex virus UL12.5M185
TagsnoneExpressionMammalianMutationN-terminal deletion of UL12.5PromoterCMV and RSVAvailable SinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MCS-ASC-GFP
Plasmid#73957PurposeExpress human ASC with a C-terminal EGFP tag in mammalian cellsDepositorInsertPYD and CARD domain containing, PYCARD (PYCARD Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterCMV IEAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cas9 sgRNA vector
Plasmid#68463PurposeCas9 sgRNA vector for cloningDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh
Plasmid#68853PurposeExpresses SMASh tag fused to YFP at C terminus in mammalian cellsDepositorInsertYFP-SMASh
TagsSMASh tagExpressionMammalianPromoterCMVAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
Plasmid#64218PurposeExpression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2ADepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-BFP-P2A-E1BExpressionMammalianPromoterCBh and U6Available SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-N1
Plasmid#54521PurposeLocalization: N1 Cloning Vector, Excitation: 462, Emission: 492DepositorTypeEmpty backboneTagsmTFP1ExpressionMammalianAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA eIF4GI (1-1599)
Plasmid#45640DepositorAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
AmCyan-P2A-mCherry
Plasmid#45350DepositorInsertAmCyan
TagsmCherryExpressionMammalianMutationSV40 NLSPromoterCMVAvailable SinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG2
Plasmid#27341DepositorInsertSFFV promoter, IRES-eGFP
UseLentiviralExpressionMammalianAvailable SinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
myc-hULK1
Plasmid#31961DepositorAvailable SinceSept. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sh1368 knockdown c-myc
Plasmid#29435DepositorAvailable SinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only