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165,839 results

Showing: 2551 - 2600 of 165839 results

  1. DHIP1

    Bacterial Strain
    #226851
    Purpose
    Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria
    Depositor
    David Nielsen
    Article
    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:
    Bacterial Resistance
    Streptomycin
    Species
    Escherichia coli
    Available Since
    Nov. 15, 2024
    Availability
    Academic Institutions and Nonprofits only

  3. pCS2+MLS-HyPer7

    Plasmid
    #136470
    Purpose
    Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Article
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Insert
    HyPer7
    Use
    Tags
    Tandem mitochondrial targeting signal of cytochro…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136470-plasmid-map-sequence-id-271540

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  4. pINDUCER dCas9-TET1CDMut

    Plasmid
    #101920
    Purpose
    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
    Depositor
    Danwei Huangfu
    Article
    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10
    Insert
    dCas9-TET1CD Mut
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Mutated TET1 catalytic domain
    Promoter
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101920-plasmid-map-sequence-id-194554

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  17. pHCA/GAL4(1-93).ER.VP16

    Plasmid
    #108216
    Purpose
    Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain
    Depositor
    Didier Picard
    Article
    Louvion et al Gene 1993 Sep 6;131(1):129-34.
    Insert
    Gal4 DBD-ERalpha HBD-VP16
    Use
    Tags
    Expression
    Yeast
    Mutation
    ERalpha HBD has G400V mutation
    Promoter
    ADH1
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108216-plasmid-map-sequence-id-208792

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  18. TFORF3532

    Plasmid
    #145008
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LIN28B (LIN28B Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145008-plasmid-map-sequence-id-287138

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  19. pG1K

    Plasmid
    #71741
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71741-plasmid-map-sequence-id-130144

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  20. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  22. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid
    #74939
    Purpose
    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    Ubiquitin
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available Since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    74939-plasmid-map-sequence-id-151347

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  35. TFORF2531

    Plasmid
    #142954
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX9 (SOX9 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    142954-plasmid-map-sequence-id-396957

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  37. mTurquoise-Rab5a-7

    Plasmid
    #55578
    Purpose
    Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Rab5a
    Use
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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Showing: 2551 - 2600 of 165839 results