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4,132 results

Showing: 1 - 40 of 4132 results

  1. pMD2.G

    Plasmid
    #12259
    Purpose
    VSV-G envelope expressing plasmid
    Depositor
    Didier Trono
    Insert
    VSV G
    Use
    Lentiviral; Envelope
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12259-plasmid-map-sequence-id-443804

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  2. psPAX2

    Plasmid
    #12260
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12260-plasmid-map-sequence-id-467229

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  3. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  4. pAdDeltaF6

    Plasmid
    #112867
    Purpose
    AAV helper plasmid
    Depositor
    James M. Wilson
    Insert
    E4, E2a and VA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    112867-plasmid-map-sequence-id-372973

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  5. pAAV2/9n

    Plasmid
    #112865
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap9
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112865-plasmid-map-sequence-id-350300

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  7. pCMV-VSV-G

    Plasmid
    #8454
    Purpose
    Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    8454-plasmid-map-sequence-id-404044

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  9. pRSV-Rev

    Plasmid
    #12253
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    Rev (rev )
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12253-plasmid-map-sequence-id-443803

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  10. pMDLg/pRRE

    Plasmid
    #12251
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12251-plasmid-map-sequence-id-433915

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  11. pAAV2/5

    Plasmid
    #104964
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes for production of serotype 5
    Depositor
    Melina Fan
    Insert
    Rep2/Cap5
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    p5
    Available since
    Oct. 2, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    104964-plasmid-map-sequence-id-364714

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  13. Tet-pLKO-puro

    Plasmid
    #21915
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21915-plasmid-map-sequence-id-326721

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  20. pAAV2/1

    Plasmid
    #112862
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap1
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112862-plasmid-map-sequence-id-421935

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  21. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  23. Cre Reporter

    Plasmid
    #62732
    Purpose
    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).
    Depositor
    Niels Geijsen
    Article
    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1
    Insert
    LoxP-DsRED-STOP-LoxP-eGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    LoxP sites
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-Alpha
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    62732-plasmid-map-sequence-id-204219

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  24. pUMVC

    Plasmid
    #8449
    Purpose
    Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2005
    Availability
    Academic Institutions and Nonprofits only
    8449-plasmid-map-sequence-id-272509

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  25. eeBxb1

    Plasmid
    #222339
    Purpose
    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells
    Depositor
    David Liu
    Article
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    222339-plasmid-map-sequence-id-473210

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  27. PB-TO-hNGN2

    Plasmid
    #172115
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172115-plasmid-map-sequence-id-448148

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  28. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52962-plasmid-map-sequence-id-322376

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  37. pPSU1

    Plasmid
    #89439
    Purpose
    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Article
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89439-plasmid-map-sequence-id-190157

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  38. pPSU2

    Plasmid
    #89566
    Purpose
    High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Article
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    750 bp EcoRV fragment
    3000 bp EcoRV fragment
    4000 bp EcoRV fragment
    50 bp PstI fragment
    100 bp PstI fragment
    200 bp PstI fragment
    300 bp PstI fragment
    400 bp PstI fragment
    500 bp PstI fragment
    600 bp PstI fragment
    1500 bp Psti fragment
    4100 bp PstI fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89566-plasmid-map-sequence-id-177206

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  39. pComb3XSS

    Plasmid
    #63890
    Purpose
    3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Inserts
    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    Use
    Phage display
    Tags
    6x His, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63890-plasmid-map-sequence-id-449948

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  40. pEcCas

    Plasmid
    #73227
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
    Depositor
    Sheng Yang
    Article
    Li et al Acta Biochim Biophys Sin (Shanghai). 202
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR; Red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native promoter
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73227-plasmid-map-sequence-id-419024

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Showing: 1 - 40 of 4132 results