19,178 results
-
-
pSL21-mCherry
Plasmid#164410PurposeRetroviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoterā¦Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3-VEGFR3
Plasmid#119230PurposePlasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.DepositorInsertVEGFR3 (FLT4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-MCS-IRES-eGFP-P2A-iCas9
Plasmid#178533PurposeFor genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cellsDepositorInsertiCaspase9 (CASP9 Human)
UseTagsExpressionMammalianMutationFKBP12 F36 to V Truncated Caspase9 (135-416; lackā¦PromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDB327
Plasmid#113671Purposeexpresses Ulp1_R3 in Escherichia coliDepositorInsertUlp1_R3
UseTags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB::Pleft* mScarlet
Plasmid#169410PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarletDepositorInsertspLeft
mScarlet proper
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-BLM
Plasmid#110299PurposeMammalian expression of a EGFP-tagged full length Bloom's syndrome helicaseDepositorInsertBLM (Bloom's Syndrome) helicase (BLM Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-SARS2-B.1.617.2
Plasmid#172320Purposeexpressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.DepositorInsertSpike of B.1.617.2 strain (S SARS-CoV-2)
UseTagsExpressionMammalianMutationT19R, 156G, 157-158del, L452R, T478K, D614G, P681ā¦PromoterCMV-FAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6sheLLr
Plasmid#37318DepositorInsertHPV6L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Human LFA-1 alpha
Plasmid#8630DepositorInsertLFA1 alpha (ITGAL Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-GW
Plasmid#61702PurposeGateway compatible Y2H vector - ADDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)
Plasmid#140659PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NlucDepositorInsertOsTIR1(F74G) mAID-EGFP-Nluc TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-GL
Plasmid#51225Purposebi-directional expression vector for yeast, galactose inducible, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGAL1,10Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTlpA36-Wasabi
Plasmid#86115PurposemWasabi reporter gene gated by TlpA36DepositorInsertsTlpA36
mWasabi
UseTags6xHisExpressionBacterialMutationD341A, P60L, D135V, K187R, K202I, L208QPromoterpTlpA and pTlpA, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cre-IRES-PuroR
Plasmid#30205PurposeLentiviral coexpression of Cre and puromycin from the human EF1a promoterDepositorInsertCre recombinase (cre Enterobacteria phage P1)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-Lux
Plasmid#109383PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coliDepositorInsertpARABAD-LUX operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Actb KI
Plasmid#131479PurposeEndogenous tagging of Ī²actin: N-terminal (amino acid position: D2)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP
Plasmid#174168PurposeLentiviral vector to constitutively express YAP under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationPromoterEFSAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR-PIP-FUCCI
Plasmid#118621PurposeGateway cloning vector for the cell cycle indicator PIP-FUCCIDepositorInsertPIP-FUCCI
UseGateway cloningTagsmCherry and mVenusExpressionMutationPromoterAvailable sinceApril 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 KRAB-T2A-GFP
Plasmid#67620PurposeCo-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFPDepositorInserthumanized dead Cas9 KRAB T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP-IRES2-EGFP
Plasmid#80333PurposeExpresses Danio rerio VSP with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC5Kan-P2A
Plasmid#51814PurposeP2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single messageDepositorInsertP2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
UseShuttle vector for insect expression vectorsTagsExpressionMutationcodons altered for Drosophila and to eliminate reā¦PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV
Plasmid#91719PurposeAAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2DepositorInsertmEGFP-P2A-paAIP2(R5AR6A)
UseAAVTagsExpressionMutationPromoterCaMKII 1.3Available sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-Membrane-1
Plasmid#57992PurposeLocalization: Plasma Membrane, Excitation: 555, Emission: 584DepositorTypeEmpty backboneUseTagsN-terminal 20 Aas MLS from Neuromodulin and mTagRā¦ExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
CRY2high-mCherry
Plasmid#104063PurposeExpresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherryDepositorInsertCRY2high-mCherry
UseTagsExpressionMammalianMutationCRY2PHR E490RPromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsEDC4
Plasmid#66597PurposeExpresses lambdaN-HA-tagged EDC4 in mammalian cellsDepositorInsertEDC4 (EDC4 Human)
UseTagslambdaN-HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nMagHigh1-EGFP-CAAX
Plasmid#67300PurposeBlue light dependent photoswitching systemDepositorInsertnMagHigh1-EGFP-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab11 WT
Plasmid#12679DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsDsRed and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184S
Plasmid#106186PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184SPromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPC-scribP305L
Plasmid#24601DepositorInsertScribble (SCRIB Human)
UseRetroviralTagsExpressionMammalianMutationP305LPromoterAvailable sinceMay 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA
Plasmid#37090PurposeExpresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitationDepositorInserthChr2(H134R)-mCherry
UseAAV and Cre/Lox; Cre-offTagsmCherryExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9-KRAB
Plasmid#110822PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86ā¦PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-DsRed
Plasmid#13769PurposeCre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.DepositorInsertDsRed2
UseCre/Lox; Flp/frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA
Plasmid#183775PurposeAAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertNLS-GFP
UseAAVTagsExpressionMutationNAPromoterCAGAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBT-PE2-PuroTK-pegRNA_GG
Plasmid#173222PurposepiggyBac transposon containing both PE2 and a pegRNA cloning site.DepositorInsertsPE2
PuroTK
pegRNA cloning cassette
UsePiggybacTagsExpressionMammalianMutationPromoterCMV, P2A, and hU6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3b-8XGliBS:EGFP
Plasmid#84602PurposeGli protein-dependent EGFP reporter for analysis of Hedgehog signalingDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoter8X Gli-binding siteAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEE2E-TRI
Plasmid#71288PurposeEgg cell-specific promoter-controlled expression 3ĆFLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPCDepositorInsertssgRNA targeting TRY and CPC genes
sgRNA targeting ETC2 gene
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 enhancer fused to EC1.1 promoter and U6-26pā¦Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPerRed-mito
Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
UseTagsMitochondrial targeting signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A
Plasmid#69290PurposeGateway entry vector; single gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP
Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC12-10XUAS-IVS-myr::GFP
Plasmid#26222DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGWB555
Plasmid#74885PurposeGateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-P2A-EGFP (RTW3027)
Plasmid#139987PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.LifeAct-Ruby.W
Plasmid#51009PurposeLentivector that expresses LifeAct-Ruby from internal human PGK promoterDepositorInsertLifeAct-mRFPruby
UseLentiviralTagsLifeActExpressionMammalianMutationPromoterhuman PGKAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-NFAT1(4-460)-GFP
Plasmid#11107PurposeExpresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
UseTags3x HA and GFPExpressionMammalianMutationContains amino acids 4-460 of NFAT1; L78P compareā¦PromoterCMVAvailable sinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf17-22
Plasmid#198272PurposeUnmodified pPayload; contains WT PVC payloads/regulatory genesDepositorInsertPVCpnf17-22
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-Neo-ISG15
Plasmid#80404Purposeoverexpression of ISG15DepositorInsertISG15 (ISG15 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK170.AAV-TRE-Cre-WPRE (Supernova)
Plasmid#85040PurposeFor AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.DepositorInsertnlsCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
plRL2
Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-hEAAT3
Plasmid#32815DepositorInserthEAAT3 (SLC1A1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only
