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pcDNA3.1-ZNF598-TEV-3xFLAG
Plasmid#105690PurposeExpresses C terminally 3xFLAG tagged ZNF598 proteinDepositorInsertZNF598 (ZNF598 Human)
UseTagsTEV-3xFLAGExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-MCS-IRES-mRFP
Plasmid#36972DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTREGFP2
Plasmid#22450DepositorInsertEGFP (eGFP )
UseTagsExpressionMutationPromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1[85Q]
Plasmid#33237DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsFlagExpressionMammalianMutationinsert contains a stretch of 85 Glutamines and no…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTP943
Plasmid#104157PurposeBacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fab specific nanobody TP886
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
7TGC
Plasmid#24304DepositorInsert7xTcf-eGFP // SV40-mCherry
UseLentiviralTagsExpressionMutationEGFP is GFP with S65T and F64L mutationsPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pU6-sp-sgRNA-RNF2_+41nick
Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorInsertRNF2_+41 nicking sgRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgRNA(F+E)-sgGal4
Plasmid#100549PurposesgGal4-expressing vector modified from Addgene plasmid #51024DepositorInsertsgGal4
UseCRISPRTagssgRNAExpressionMammalianMutationPromoterU6Available sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pseudojanin (PJ)
Plasmid#37999DepositorUseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-AML1B
Plasmid#12426PurposepCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.DepositorInsertAML1B (RUNX1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-Cul7
Plasmid#20695DepositorInsertcullin 7 (CUL7 Human)
UseTagsMyc3ExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pH2B_mCherry_IRES_neo3
Plasmid#21044DepositorInsertpH2B
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG4/κ
Plasmid#61887PurposeExpresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-H2BtdiRFP-IP
Plasmid#47884PurposeHistone H2B fused with tandem iRFP, ires-puroDepositorInsertH2B-tdiRFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX304-FOS-V5
Plasmid#59140Purposewild-type FOS with C-terminal V5 tag expressed from pLX vectorDepositorInsertFOS (FOS Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
PJ-DEAD
Plasmid#38002DepositorUseTagsmRFPExpressionMammalianMutationAsp 1263 mutated to Ala and Cys 779 mutated to SerPromoterAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterGalLAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
mADAM17 in pcDNA3.1
Plasmid#19141DepositorInsertADAM17 (Adam17 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
BirA(R118G)-HA destination vector
Plasmid#53581PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)DepositorTypeEmpty backboneUseTagsBirA(R118G) and HA tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Hygro DEST (693-2)
Plasmid#17291Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, HygroDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP2SA11
Plasmid#89163PurposeExpress Rotavirus SA11 VP2 in mammalian cellsDepositorInsertRotavirus SA11 VP2
UseTagsExpressionMammalianMutationPromoterT7 promotorAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP1SA11
Plasmid#89162PurposeExpress Rotavirus SA11 VP1 in mammalian cellsDepositorInsertRotavirus SA11 VP1
UseTagsExpressionMammalianMutationPromoterT7 promotorAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-L11
Plasmid#20936DepositorInsertRibosomal protein L11 (RPL11 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PE2-BSD
Plasmid#161514PurposeExpression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selectionDepositorInsertPE2-P2A-BSD
UseLentiviralTagsSV40 NLSExpressionMammalianMutationPromoterEF-1α core promoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEos4b-C1
Plasmid#54812PurposeLocalization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneUseTagsmEos4bExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP×2-N1
Plasmid#86775PurposeA mammalian expression vector with double EGFP at C-terminus.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)
Plasmid#44028DepositorInsertmHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
UseTagsMycExpressionMammalianMutationP402A, P577A, N813APromoterCMVAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha-wt-pBabe-puro
Plasmid#19365DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwt human cDNAPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-mCherry
Plasmid#75161PurposeDerived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-YFP II (pMIY II)
Plasmid#52108Purposea bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorTypeEmpty backboneUseRetroviralTagsIRES-EYFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
E. coli groE operon
UseTagsExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-rev-ChR2-tdtomato
Plasmid#18917PurposeCre-dependent viral expression of ChR2-tdTomato for neuronal excitationDepositorInsertChannelrhodopsin 2-tdtomato
UseAAV and Cre/Lox; Adeno-associated virusTagstdtomatoExpressionMutationPromoterCAGAvailable sinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eNpHR 3.0-EYFP
Plasmid#26972PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibitionDepositorHas ServiceAAV5InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHsp70-Cas9
Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorInsertCodon optimized Cas9
UseCRISPRTags3x FLAGExpressionMutationSilent change from C to A at bp 834 of insertPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cerulean-N1
Plasmid#54742PurposeLocalization: N1 Cloning Vector, Excitation: 433, Emission: 475DepositorTypeEmpty backboneUseTagsCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNAFLAG-ATM-kd
Plasmid#43908DepositorInsertataxia telangiectasia mutated (ATM Human)
UseTagsFLAG and HisExpressionMammalianMutationkinase dead (D2870A and N2875K)PromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSADdeltaG-GFP-F2
Plasmid#32635DepositorInsertGFP
UseRabies virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab35 WT
Plasmid#47424DepositorInsertRab35 (RAB35 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-flex-dsRed-shvgat
Plasmid#67845PurposeExpresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switchDepositorInsertAAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
UseAAV, Cre/Lox, Mouse Targeting, and RNAiTagsExpressionMammalianMutationPromoterh-synapsinAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only
