19,186 results

1. pDisplay-R-iGluSnFR1

   Plasmid

   #107335

   Purpose

   Red fluorescent glutamate sensor based on cpmApple. Membrane-displayed

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

   Insert

   R-iGluSnFR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA3.3_CoV2_B.1.1.7

   Plasmid

   #170451

   Purpose

   Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Yuan et al Science. 2021 Aug 13;373(6556):818-823.

   Insert

   SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

   Promoter

   CMV

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLJC6-GLS-3xFLAG

   Plasmid

   #163450

   Purpose

   lentiviral overexpression vector for human GLS

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   GLS (GLS Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   Ubc

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Delta(Pro-MP)ADAM17-HA

   Plasmid

   #65221

   Purpose

   Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   Delta(Pro-MP)ADAM17 (ADAM17 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted AA 18-473

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28HIS-hAPE1

   Plasmid

   #70757

   Purpose

   bacterial expression of human APE1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   APEX1 (APEX1 Human)
   

   Use

   Tags

   6-HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lac operon

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. IKK-2 K44M

   Plasmid

   #11104

   Depositor

   Anjana Rao

   Article

   Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

   Insert

   IKK-2 (IKBKB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K44M, kinase inactive

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZ8-T_dCas9

   Plasmid

   #74062

   Purpose

   pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   dcas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-1xMyc)

   Plasmid

   #160368

   Purpose

   Stable expression of GPR143-mScarlet-1xMyc fusion protein for use as a control with MelanoTag-PURO

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   GPR143 (GPR143 Human)
   

   Use

   Lentiviral

   Tags

   mScarlet-myc

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5/FRT/TO V5

   Plasmid

   #19445

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   V5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V5 tag inserted to create new vector

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMBP-MS2

    Plasmid

    #65104

    Purpose

    can be used to isolate RNA binding complexes

    Depositor

    Josep Vilardell

    Article

    Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

    Insert

    MS2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    double mutation (V75Q and A81G) that prevents oli…

    Promoter

    tac

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Bmi1-overexpression

    Plasmid

    #21577

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Insert

    Bmi-1 (Bmi1 Mouse)
    

    Use

    Lentiviral

    Tags

    ires-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-N1 alpha-actinin 1

    Plasmid

    #11908

    Depositor

    Carol Otey

    Article

    Edlund et al Cell Motil Cytoskeleton. 2001 Mar . 48(3

    Insert

    alpha-actinin 1 (ACTN1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-2T-Rac1-Q61L

    Plasmid

    #12979

    Depositor

    Gary Bokoch

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

14. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-CamKIIa-jGCaMP8f-WPRE

    Plasmid

    #176750

    Purpose

    AAV-mediated expression of GCaMP8f under the CamKIIa promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIalpha

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBb(RK2)1k-GFPuv

    Plasmid

    #106394

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on RK2 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMRXIP GFP-Stx17 WT

    Plasmid

    #45909

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AmCyan1-N1

    Plasmid

    #54616

    Purpose

    Localization: N1 Cloning Vector, Excitation: 453, Emission: 486

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    AmCyan1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAnc80L65

    Plasmid

    #68837

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

    Depositor

    Luk Vandenberghe

    Article

    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

    Insert

    Ancestral AAV Capsid Anc80L65
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Rep2

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti PGK V5-LUC Puro (w543-1)

    Plasmid

    #19360

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSF-MCM3/5

    Plasmid

    #116950

    Purpose

    Expresses human MCM3 and human MCM5

    Depositor

    James Chong

    Article

    Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

    Inserts

    Human MCM5 (MCM5 Human)
    human MCM3 (MCM3 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid

    #71707

    Purpose

    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

    Depositor

    Giulio Draetta

    Article

    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

    Insert

    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    

    Use

    CRISPR

    Tags

    3xFLAG and hGEM(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HIVGKO

    Plasmid

    #112234

    Purpose

    HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

    Depositor

    Eric Verdin

    Article

    Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

    Insert

    csGFP-EF1a-mKO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentiPGK Puro DEST JNKKTRClover

    Plasmid

    #59151

    Purpose

    Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. En1

    Plasmid

    #34993

    Depositor

    Malin Parmar

    Article

    Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

    Insert

    Homeobox protein engrailed-1 (En1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNLS-iRFP720

    Plasmid

    #45467

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 3xFLAG-CUL1-pCMV7.1

    Plasmid

    #155019

    Purpose

    N-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 1 (CUL1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

    Plasmid

    #205555

    Purpose

    Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pICH41780

    Plasmid

    #48019

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pcDNA3-ExRai-AMPKAR(T/A)

    Plasmid

    #192447

    Purpose

    Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR(T/A)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Thr 6 in AMPK substrate mutated to Ala.

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBluescript-PB

    Plasmid

    #133885

    Purpose

    piggyBac ITR's in high copy number pBluescript backbone plasmid

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

    Plasmid

    #132406

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pShuttle-CMV-iRFP

    Plasmid

    #31856

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Insert

    iRFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFB1_hMSH2

    Plasmid

    #129423

    Purpose

    Expression human MSH2 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH2 (MSH2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mcherry-PH

    Plasmid

    #36075

    Depositor

    Narasimhan Gautam

    Article

    Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

    Insert

    PH domain of PLCdelta1 (PLCD1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAGGS_VP35_EBOV

    Plasmid

    #103050

    Purpose

    Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK-Myc-Smurf1

    Plasmid

    #13676

    Depositor

    Ying Zhang

    Article

    Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

    Insert

    Smurf1 (SMURF1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCas9-Geminin

    Plasmid

    #109401

    Purpose

    Expresses Cas9 fusion to Geminin degron domain

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    Geminin (GMNN Human)
    

    Use

    Tags

    Expression

    Mutation

    Geminin degron fragment

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. proE-cad178-Luc

    Plasmid

    #42081

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    E-cadherin (CDH1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    contains E-cadherin promoter region from -178 to …

    Promoter

    E-cadherin

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. DNA-PKcs ABCDE>A

    Plasmid

    #83318

    Purpose

    human DNA-PKcs/ABCDE>ala

    Depositor

    Katheryn Meek

    Article

    Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    6 phosphorylation sites in the ABCDE cluster subs…

    Promoter

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Rabbit GFP Cav 2.2 pMT2

    Plasmid

    #58737

    Purpose

    expression of GFP tagged rabbit Cav2.2 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

    Insert

    Cav2.2 (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. EasyFusion T2A-H2B-miRFP703

    Plasmid

    #113097

    Purpose

    General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-miRFP703
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Human ICAM-2

    Plasmid

    #8634

    Depositor

    Timothy Springer

    Article

    Staunton et al Nature 1989 May 4;339(6219):61-4.

    Insert

    ICAM-2 (ICAM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

45. shh10

    Plasmid

    #64867

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

    Insert

    shh10 cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    I319V, N451D, D532N

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. anxA2-GFP

    Plasmid

    #107196

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    annexin A2 (ANXA2 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Ala residue 65 in human sequence replaced by Glu …

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

    Plasmid

    #126679

    Purpose

    Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

    Depositor

    Hee Won Yang

    Article

    Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

    Insert

    Rb (a.a 886-928)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCutamp

    Plasmid

    #140632

    Purpose

    Plasmid-curing in Escherichia coli by targeting the AmpR promoter

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pEYFP-Mitotrap

    Plasmid

    #46942

    Purpose

    Expresses Mitotrap in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Mitotrap (MTOR Aequorea victoria, Budding Yeast, Synthetic, Human)
    

    Use

    Tags

    This is a fusion between a yeast mitochondrial ta…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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50. plentiCMV-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196511

    Purpose

    Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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