165,343 results

1. LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

   Plasmid

   #60629

   Purpose

   Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   VP64 human MyoD (MYOD1 Human)
   

   Use

   Lentiviral

   Promoter

   TRE

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-hG9a

   Plasmid

   #33025

   Depositor

   Martin Walsh

   Article

   Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

   Insert

   hG9a (EHMT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-OVA

   Plasmid

   #64599

   Purpose

   Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

   Depositor

   Sandra Diebold, Martin Zenke

   Article

   Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

   Insert

   Ovalbumin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

   Plasmid

   #110854

   Purpose

   Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   sgRNA scaffold with spacer
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   U6

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRIS-PITChv2-Puro-dTAG (BRD4)

   Plasmid

   #91793

   Purpose

   PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   Puro-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. BASU RaPID plasmid

   Plasmid

   #107250

   Purpose

   Expresses the BASU RaPID labeling protein

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   BASU
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro

   Plasmid

   #234070

   Purpose

   For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_CMV:Flag-Antares

   Plasmid

   #208852

   Purpose

   Expresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

   Insert

   Antares
   

   Use

   Lentiviral

   Tags

   1xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-EGFP-Cdc42-T17N

   Plasmid

   #12976

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Cdc42 DN (CDC42 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Promoter

   CMV

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-TetON-Sox10

    Plasmid

    #115242

    Purpose

    Lentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5E-mpeg1.1

    Plasmid

    #75023

    Purpose

    Can be used to drive expression in zebrafish microglia.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Promoter

    mpeg1.1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUBC-popTag-QtE-mScarlet

    Plasmid

    #240101

    Purpose

    Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.

    Depositor

    Liam Holt

    Article

    Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e

    Insert

    Encapsulin
    

    Use

    Lentiviral

    Tags

    mScarlet and popZ

    Expression

    Bacterial and Mammalian

    Promoter

    UBC promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEL685

    Plasmid

    #205592

    Purpose

    NEPE for prime editing in rice

    Depositor

    Yong Zhang

    Article

    Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    ZmUbi

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-PE2-His (IK1822)

    Plasmid

    #170103

    Purpose

    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

    Depositor

    Keith Joung

    Article

    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

    Insert

    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    

    Use

    CRISPR

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Bacteria codon-optimized nSpCas9 (H840A) & M-…

    Promoter

    T7

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

    Plasmid

    #200400

    Purpose

    Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

    Depositor

    Daniel Kronauer

    Article

    Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

    Inserts

    Discosoma red fluorescent protein
    Q factor 2
    GCaMP6s
    

    Expression

    Insect

    Promoter

    15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAS-V5-miniTurboID-NES

    Plasmid

    #116905

    Purpose

    Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurboID
    

    Use

    Drosophila expression

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a_TIA1

    Plasmid

    #106095

    Purpose

    bacterial protein expression of TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Tags

    6xHIs-thrombin and T7

    Expression

    Bacterial

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOS003-lenti-CMV-GCaMPer

    Plasmid

    #65227

    Purpose

    ER calcium sensor for lentivirus production

    Depositor

    Brandon Harvey, Adam Cohen

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    circularly permuted GFP

    Expression

    Mammalian

    Mutation

    GCaMP3 (D324G, D360G, D397G, D435G)

    Promoter

    CMV

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-BRD4

    Plasmid

    #183939

    Purpose

    Transcriptional co-activator BRD4 labeled by mCherry

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    mCherry-BRD4 (Brd4 Mouse, Synthetic)
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available Since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. AAV-DIO-TC66T-2A-EGFP-2A-oG

    Plasmid

    #178429

    Purpose

    Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

    Depositor

    Edward Callaway

    Inserts

    TVA950 Glu66→Thr
    Optimized G
    EGFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Promoter

    hSyn

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCK017 pDEST exorh:EGFP

    Plasmid

    #195983

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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24. RMCE wild type DNA-PKcs

    Plasmid

    #233911

    Purpose

    Low copy number provides expression of human DNA-PKcs

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    dna-pkcs cDNA (PRKDC Human)
    

    Expression

    Mammalian

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid

    #84563

    Purpose

    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    

    Use

    Lentiviral

    Tags

    myc tag (9x)

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3-endo-Nb37

    Plasmid

    #205514

    Purpose

    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb37
    

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP VAMP8

    Plasmid

    #42311

    Depositor

    Thierry Galli

    Article

    Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

    Insert

    VAMP 8 (VAMP8 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV2-DEST(r)

    Plasmid

    #190238

    Purpose

    Gateway destination vector, empty AAV vector backbone

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DNA-PKcs T3950D

    Plasmid

    #83320

    Purpose

    DNA-PKcs T3950A

    Depositor

    Katheryn Meek

    Article

    Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    Activation loop phosphorylation site 3950 substit…

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDEST exorh:mCherry

    Plasmid

    #195989

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Nicolas Rohner

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. RMCE halo-flag DNA-PKcs

    Plasmid

    #233918

    Purpose

    Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Tags

    flag-halo

    Expression

    Mammalian

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-EGFP-Cdc42-Q61L

    Plasmid

    #12986

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1-3XHA-p53

    Plasmid

    #196267

    Purpose

    Mammalian expression of 3xHA tagged wild-type p53

    Depositor

    Laura Attardi

    Article

    Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

    Insert

    p53 (Trp53 Mouse)
    

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLVX-M-puro

    Plasmid

    #125839

    Purpose

    modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TRE3G-PE2-P2A-BFP

    Plasmid

    #231580

    Purpose

    Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

    Depositor

    Jesse Engreitz

    Article

    Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

    Insert

    PE2-P2A-BFP
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSems LifeAct-StayGold

    Plasmid

    #222947

    Purpose

    Expression of LifeAct tagged with StayGold in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    LifeAct
    

    Tags

    StayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid

    #101893

    Purpose

    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Promoter

    CMV immediate early

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

    Plasmid

    #85424

    Purpose

    Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    anti-Her2_4D5-7_synNotch
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBABE-puro-hTERT-HA

    Plasmid

    #1772

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    2XHA

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #49425

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

    Depositor

    Daniel Klionsky

    Article

    Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Promoter

    ATG8

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge