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We narrowed to 21,531 results for: SPR

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Showing: 881 - 920 of 21531 results

  12. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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  13. pDD285

    Plasmid
    #66826
    Purpose
    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    mKate2-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized mKate2
    Expression
    Worm
    Available Since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87844-plasmid-map-sequence-id-174620

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  28. AICSDP-7:SEC61B-mEGFP

    Plasmid
    #87426
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87426-plasmid-map-sequence-id-173967

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  30. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Article
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98592-plasmid-map-sequence-id-201329

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  32. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid
    #74939
    Purpose
    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    Ubiquitin
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available Since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    74939-plasmid-map-sequence-id-151347

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  38. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid
    #99248
    Purpose
    single-vector system with EGFP reporter for use in CROPseq knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99248-plasmid-map-sequence-id-191832

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  40. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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Showing: 881 - 920 of 21531 results