163,570 results

1. AAV.CAG.GLPLight1

   Plasmid

   #187467

   Purpose

   Expresses GLPLight1 in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

   Insert

   GLPLight1
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Galphai2-mTurquoise2

   Plasmid

   #69621

   Purpose

   The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   Galphai2-mTurquoise2
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-N-Myc_C223S USP7

   Plasmid

   #131243

   Purpose

   Mammalian expression of catalytically inactive Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a point mutation that converts USP7 cyst…

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV_BiPVe4_ChR2_mCherry

   Plasmid

   #213937

   Purpose

   AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB and AAV1

   Insert

   Channelrhodopsin 2-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHCA/GAL4(1-93).ER.VP16

   Plasmid

   #108216

   Purpose

   Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

   Depositor

   Didier Picard

   Article

   Louvion et al Gene 1993 Sep 6;131(1):129-34.

   Insert

   Gal4 DBD-ERalpha HBD-VP16
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ERalpha HBD has G400V mutation

   Promoter

   ADH1

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF3532

   Plasmid

   #145008

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LIN28B (LIN28B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNTI194

   Plasmid

   #84266

   Purpose

   Expresses Avi-tagged RPL1 and humanized birA

   Depositor

   Nicholas Ingolia

   Article

   Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

   Inserts

   RPL1 (RPL10A Human, (*see below))
   humanized birA
   

   Use

   Tags

   Avi tag, Flag, Gly/Ser linker, and TEV site

   Expression

   Mammalian

   Mutation

   R33A, A34E (*see below)

   Promoter

   CMV and P2A peptide

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pG1K

   Plasmid

   #71741

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Type

   Empty backbone

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BB3cK_pGAP_23*_pTEF_Cas9

   Plasmid

   #104909

   Purpose

   hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

    Plasmid

    #132406

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid

    #74939

    Purpose

    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    Ubiquitin
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    UbvG08 with I44A mutation and no terminal Glycines

    Promoter

    CMV

    Available Since

    June 30, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pEGFP-N1-human lyn–GFP

    Plasmid

    #35958

    Purpose

    Expresses human lyn protein codon optimized for zebrafish expression.

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

    Insert

    LYN (LYN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    cmv

    Available Since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV_BiPVe3_ChR2_mCherry

    Plasmid

    #213941

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer. Alias: AAV_WDP0003_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HA-14-3-3 gamma

    Plasmid

    #13274

    Depositor

    Michael Yaffe

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Pseudojanin (PJ)

    Plasmid

    #37999

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    FKBP1A (FKBP1A Human)
    SAC1
    INPP5E (INPP5E Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJMP3

    Plasmid

    #79875

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into thrC

    Depositor

    Stanley Qi, Carol Gross, Jason Peters

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW-TetON-GFP

    Plasmid

    #115495

    Purpose

    Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207861

    Purpose

    AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD/His-miRFP670nano3

    Plasmid

    #184663

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano3 in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    araC

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV-YFP

    Plasmid

    #26000

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-YFP (H2BC21 Human, A. victoria)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mTurquoise-Rab5a-7

    Plasmid

    #55578

    Purpose

    Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Use

    Tags

    mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pDEST-ORF-V2

    Plasmid

    #73638

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Galphai3-mTurquoise2

    Plasmid

    #69622

    Purpose

    The heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    Galphai3-mTurquoise2
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF2531

    Plasmid

    #142954

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX9 (SOX9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAW016-2

    Plasmid

    #85613

    Purpose

    Cas9-tracrRNA integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    cas9-tracrRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSC101-pT7-T3RNAP

    Plasmid

    #140872

    Purpose

    T7 promoter driven synthesis of T3 RNA polymerase

    Depositor

    Neal Devaraj

    Article

    Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

    Insert

    T3 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-Flag-Tet2 CD

    Plasmid

    #72219

    Purpose

    Express flag-tagged mouse Tet2 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 2 (Tet2 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET2 catalytic domain.

    Promoter

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

29. LYN11-FRB-mcherry

    Plasmid

    #38004

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    LYN (LYN Human)
    MTOR (MTOR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.pU1a-SpCas9

    Plasmid

    #121507

    Purpose

    Expresses codon-optimized SpCas9 in mammalian cells.

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    SpCas9
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. amilCP_Pink chromoprotein

    Plasmid

    #117851

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP_Pink
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    64A,65C, BioBrick sites removed

    Promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNCS YFP

    Plasmid

    #91759

    Purpose

    Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

    Insert

    Venus YFP (A206K)
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PxylA

    Plasmid

    #55178

    Purpose

    Xylose-inducible promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    xylA promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    xylA

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIG-602_NY-ESO-1_TCR_RFP

    Plasmid

    #207483

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA5/FRT/TO V5 HSPA8

    Plasmid

    #19514

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 8 (HSPA8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. hFerritinL

    Plasmid

    #122653

    Purpose

    Expression of hFerritinL in bacterial cells

    Depositor

    Trevor Douglas

    Insert

    light chain ferritin, human (FTL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-hSyn-DIO-GRAB-gDA3m

    Plasmid

    #208707

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    MLV LTR

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMSCV-IRES-GFP/CREB3L1

    Plasmid

    #214767

    Purpose

    Mammalian expression of human CREB3L1

    Depositor

    Norio Komatsu

    Article

    De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

    Insert

    CREB3L1 (CREB3L1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-TRE-eYFP

    Plasmid

    #104056

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge