We narrowed to 3,043 results for: beta-actin
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Plasmid#60659PurposeLabels synaptic vesicles for electron microscopyDepositorInsertVAMP2:HRP
UseAAV and Cre/LoxTagsMycPromoterCAGAvailable SinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
tol2-CAG::ChR2-YFP
Plasmid#59740PurposeOptogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)DepositorInsertChannelrhodopsin-2
TagsYFPExpressionMammalianMutationH134RPromoterCAGAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GBP1-10gly-lexADBD
Plasmid#50795PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP1-lexA DNA binding domain fusion protein
ExpressionMammalianPromoterCAGAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTALYM4SpMi-01
Plasmid#47879PurposeTALE-mRuby2 against Mus spretus minor satellite sequence containing two SNPsDepositorInsertTALE-mRuby2
UseTALENTagsmRuby2ExpressionMammalianPromoterCAGAvailable SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTALYM9B15
Plasmid#47877PurposeTALE-Ty1 against mouse major satellite sequenceDepositorInsertTALE-Ty1
UseTALENTagsTy1ExpressionMammalianPromoterCAGAvailable SinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
mouse a5 full length
Plasmid#217810PurposeFull length mouse integrin a5 expressionDepositorAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLO44.1_dProtease
Plasmid#121631PurposeCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1DepositorInsertTEV protease
ExpressionMammalianPromoterU6 and chicken beta-actin promoterAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBa.NgCAM-mCherry
Plasmid#180248PurposeMammalian expression of NgCAMDepositorInsertNgCAM
ExpressionMammalianMutationWTPromoterChicken Beta actinAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCH61 (multiAsCas12a-KRAB piggyBac)
Plasmid#217332PurposepiggyBac expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Sox2AV-T2A-Klf4-E2A-Myc
Plasmid#210017Purposeepisomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.DepositorExpressionMammalianMutationSox2A61V is a point mutant of Sox2 that enhances …PromoterCAGAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).cpSFGFP.HaloTag
Plasmid#209654PurposeNon-responsive controlDepositorInsertcpSFGFP.HaloTag
UseAAVPromoterCAGAvailable SinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag
Plasmid#209652PurposeExpression of iATPSnFR2, medium affinityDepositorInsertiATPSnFR2.A95K.HaloTag
UseAAVMutationA95KPromoterCAGAvailable SinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-pAceR-Kv2.1PR
Plasmid#195534PurposeCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCAGAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17
Plasmid#193298Purposeepisomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCsDepositorExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-pur-CAG-Bi-DREADD
Plasmid#159457PurposeAAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection geneDepositorInsertBi-DREADD
ExpressionMammalianMutationfusion protein of hM3Dq-mCherry and HA-KORD seper…PromoterCAGAvailable SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Cytbow
Plasmid#158991PurposeMulticolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)DepositorInsertCytbow
TagsThe default expressed EBFP2 is fused to H2BExpressionMammalianPromoterCAGAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-NLS
Plasmid#140900Purposemammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-NLS
TagsmRFPExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Tet-on Advanced -BGH-PA
Plasmid#114379PurposeExpress reverse tetracycline inducible rtTA transcriptional activatorDepositorInsertrtTA and BGH-pA
ExpressionMammalianPromoterCAGAvailable SinceDec. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLInChR-mVenus
Plasmid#119298PurposeFLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitorDepositorInsertNx1BTM-FCS-ChR2ET/TC-mVenus
UseAAVTagsmVenusPromoterCAGAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gso (Ser)
Plasmid#109353PurposeGsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGso chimera
TagsNoneExpressionMammalianMutationChanged Cysteine at position 391 to Serine (Pertu…PromoterCAGAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-CAG-fGFP
Plasmid#108714PurposeTransposon donor plasmid for long-term expression of membrane targetted GFPDepositorInsertEGFP
Tags6xHis and farnesylation signalExpressionMammalianPromoterCAGAvailable SinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3
Plasmid#106205PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVMutationGltI: A184V + mRuby3 fusionPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-EYFP-P2A-cMaf
Plasmid#98174PurposeRetroviral vector encoding Cre-dependent expression of EYFP-P2A-MafDepositorAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9
Plasmid#89995PurposeExpresses high levels of WT Cas9 for genome editingDepositorInserthCas9
Tags3xFlag, SV40 NLS, and T2A peptideExpressionMammalianPromoterCAGAvailable SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPb-FKBP-post mGRASP
Plasmid#73146PurposeSmall fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.DepositorInsertsHRPb-FKBP-post mGRASP
TagsHA tagExpressionMammalianPromoterCAGAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFPmG1
Plasmid#50444PurposeExpression of a EGFP variant in mammalian cellsDepositorInsertGFPmG1
ExpressionMammalianMutationE143K, N147QPromoterCAGAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-SBP-DICER1
Plasmid#50558Purposepurification of Dicer1 from mammalian cellsDepositorAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-Fab-LC
Plasmid#230063PurposeExpresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.DepositorInsertAnti-GluA2 Fab light chain fragment
ExpressionMammalianPromoterChicken beta actin (CAG)Available SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP
Plasmid#89061PurposeAAV-gRNA targeting the murine Ldlr geneDepositorInsertEmGFP
UseAAV and CRISPRExpressionMammalianPromoterChicken beta actin with partial CMV enhancerAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-ARID1A-FLAG
Plasmid#242209PurposeExpression of full-length human ARID1A using piggyBac transpositionDepositorAvailable SinceSept. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216246PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integrationDepositorInsertCas9 and sgAAVS1.5
UseCRISPR; Human safe harbor locus (a avs1) site-spe…ExpressionMammalianPromoterCAGAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-GFP-4x6T
Plasmid#196418PurposeCre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertGFP
UseAAV and Cre/Lox; Astrocyte-selectiveExpressionMammalianPromoterCAGAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR
Plasmid#157744Purposeknock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-SpCas9-NG
Plasmid#171370PurposeSpCas9-NG-mediated genome editing.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6 for sgRNA; CBh for Cas9-2A-puroRAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-GCaMP7f
Plasmid#135539PurposeEncodes a specific PSD-95 binder (Xph20) fused to GCaMP7fDepositorInsertXph20
TagsGCaMP7fExpressionMammalianPromoterCAGAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Nucbow
Plasmid#158992PurposeMulticolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
TagsAll FPs are fused to H2BExpressionMammalianPromoterCAGAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-CreERT2
Plasmid#149437PurposeROSA26 targetting vector with tamoxifen-inducible Cre recombinaseDepositorInsertCre-ERT2 fusion protein
UseCre/Lox and Mouse TargetingTagsERT2ExpressionMammalianPromoterCAG promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HASAP
Plasmid#138325Purposechemigenetic voltage indicatorDepositorInsertHASAP
ExpressionMammalianPromoterCAGAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
KA1153_pPBCAG-MerCreMer-IN
Plasmid#124183PurposepiggyBac-based expression of the tamoxifen-inducible CreDepositorInsertMerCreMer-IRES-Neo
ExpressionMammalianPromoterCAGAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA0637_pPBCAG-rtTAM2-IN
Plasmid#124166PurposepiggyBac-based expression of rtTA-M2DepositorInsertrtTAM2-IRES-Neo
ExpressionMammalianPromoterCAGAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR65Q
Plasmid#92249PurposeHTT donor DNA with 65Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorMutationBarcode of wobble bases to prevent TALEN cleaving…Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-F14F15S-Switch
Plasmid#99733PurposeFlp-based FLEX reporter (from RFP to GFP)DepositorInsertTagRFP and EGFP
ExpressionMammalianPromoterCAGAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ:F3-CAGGS GPHTK-F
Plasmid#112666PurposeGenome engineering donor vector for creating master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locusDepositorInserteGFP
UseGene targeting vector for integration at the s1 s…ExpressionMammalianPromoterCAGAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF3-FLAG-IRES-GFP
Plasmid#107388PurposeLentiviral expression of IKZF3-FLAGDepositorAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG MCP-VP64
Plasmid#92357PurposeCAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.DepositorInsertMCP-VP64
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI Cas9 H2B-RFP
Plasmid#92397PurposeCAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRESDepositorInsertCas9
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc IRES neo
Plasmid#98294PurposeFor constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance geneDepositorInsertsFirefly luciferase
IRES
Neomycin resistance gene (NeoR)
UseLentiviral and LuciferaseAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP
Plasmid#86987PurposeFor cloning and expression of sgRNA together with expression of Cas9 and TagRFPDepositorInsertCas9
ExpressionMammalianPromoterCAGAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-CAG-CyRFP1
Plasmid#84357PurposeExpresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)DepositorInsertCyRFP1
UseAAVExpressionMammalianMutationDouble FLEXedPromoterCAGAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCX-Klf4
Plasmid#66656PurposeFor expression of mouse Klf4.DepositorAvailable SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only