4,132 results

1. pU6-tmpknot-GG-acceptor

   Plasmid

   #174039

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-DIO-EGFP

   Plasmid

   #50457

   Purpose

   Double floxed EGFP under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, and AAV8

   Insert

   EGFP
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-mCherry

   Plasmid

   #128744

   Purpose

   Expresses mCherry in mammalian cells

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSBbi-Pur

   Plasmid

   #60523

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pink Flamindo

   Plasmid

   #102356

   Purpose

   biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

   Insert

   Pink Flamindo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_ABEmax

   Plasmid

   #112095

   Purpose

   A:T-to-G:C base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   ABEmax
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEXTK-Pak1 70-117

   Plasmid

   #12217

   Depositor

   Jonathan Chernoff

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   PBD (p21-binding domain), aa 70-117

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBbi-RP

   Plasmid

   #60513

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-Mito-7

   Plasmid

   #55102

   Purpose

   Localization: Mitochondria, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HRE-luciferase

    Plasmid

    #26731

    Purpose

    Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

    Depositor

    Navdeep Chandel

    Article

    Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

    Insert

    Hypoxia Responsive Elements (3)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Tet-pLKO-neo

    Plasmid

    #21916

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-Frankenbody-FLAG-mEGFP

    Plasmid

    #175293

    Purpose

    Track mature and nascent FLAG tagged proteins in live cells

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. VSV.G

    Plasmid

    #14888

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    VSV-G Envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiRhoA2G

    Plasmid

    #40179

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSOUP

    Plasmid

    #165419

    Purpose

    The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

    Insert

    Rep A
    

    Use

    Helper plasmid required for replication for all p…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti6/V5-p53_wt p53

    Plasmid

    #22945

    Purpose

    3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SV40 1: pBSSVD2005

    Plasmid

    #21826

    Depositor

    David Ron

    Insert

    SV40 Large T (SV40gp6 simian virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    likely expresses aa109-708 of the large T antigen

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-Rab7A Q67L

    Plasmid

    #28049

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    Available since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. lenti-sgRNA puro

    Plasmid

    #104990

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti6.2-ccdB-3xFLAG-V5

    Plasmid

    #87071

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-15F11-HA-mCh

    Plasmid

    #129591

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRL623

    Plasmid

    #58494

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Insert

    encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti CMV GFP Hygro (656-4)

    Plasmid

    #17446

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Lentiviral Prep

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGP-CMV-GCaMP6m

    Plasmid

    #40754

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6m
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCherry2-C1

    Plasmid

    #54563

    Purpose

    Localization: C1 Cloning Vector, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LRRK2-WT

    Plasmid

    #17609

    Depositor

    Ted Dawson

    Article

    West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mRFP-Lact-C2

    Plasmid

    #74061

    Purpose

    Fluorescent biosensor for phosphatidylserine.

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 (MFGE8 Bovine)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAV-U6+27-Tornado-Broccoli

    Plasmid

    #124360

    Purpose

    Overexpression of circular RNA (Broccoli)

    Depositor

    Samie Jaffrey

    Article

    Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

    Insert

    Tornado-Broccoli
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRL443

    Plasmid

    #70261

    Purpose

    Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 7TFC

    Plasmid

    #24307

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pC013 - Twinstrep-SUMO-huLwCas13a

    Plasmid

    #90097

    Purpose

    Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a
    

    Use

    CRISPR

    Tags

    6xHis-Twin Strep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #102796

    Purpose

    Retrovirus for delivery of one sgRNA (empty, bbsI)

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    U6 sgRNA
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 4xNFkB Luc

    Plasmid

    #111216

    Purpose

    reporter gene

    Depositor

    Johannes A. Schmid

    Insert

    firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. a1Ha-pcDNA3

    Plasmid

    #45809

    Depositor

    Edward Perez-Reyes

    Article

    Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

    Insert

    a1Ha (CACNA1H Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge