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179,521 results

Showing: 1361 - 1400 of 179521 results

  1. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Publication
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98211-plasmid-map-sequence-id-485072

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  7. GFP-UtrCH

    Plasmid
    #26737
    Purpose
    Calponin homology domain of utrophin (Utr-CH) fused to EGFP in order to visualize F-actin
    Depositor
    William Bement
    Publication
    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Utrophin
    Use
    Reporter for f-actin
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Calponin homology domain of utrophin (aa# 1-261).…
    Promoter
    CMV IE94
    Available since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26737-plasmid-map-sequence-id-123090

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  11. pCMV-PEmax-P2A-BSD

    Plasmid
    #174821
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-BSD
    Tags
    P2A-BSD, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174821-plasmid-map-sequence-id-343626

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  12. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid
    #145145
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Tags
    8his tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145145-plasmid-map-sequence-id-324821

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  13. pRepCap6

    Plasmid
    #110770
    Purpose
    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.
    Depositor
    David Russell
    Publication
    Rutledge et al J Virol. 1998 Jan;72(1):309-19.
    Insert
    AAV6 cap, AAV6 rep genes
    Use
    AAV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110770-plasmid-map-sequence-id-211461

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  20. pCS2TAL3-RR

    Plasmid
    #37276
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)
    Depositor
    David Grunwald
    Publication
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    37276-plasmid-map-sequence-id-46873

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  28. hTLR4

    Plasmid
    #13086
    Depositor
    Ruslan Medzhitov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TLR4 (TLR4 Human)
    Expression
    Mammalian
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13086-plasmid-map-sequence-id-142442

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  32. pSF3-ultraID

    Plasmid
    #172878
    Purpose
    expresses ultraID in mammalian cells with the tet-on or tet-off system
    Depositor
    Julien Béthune
    Publication
    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1
    Insert
    ultraID
    Use
    Luciferase and Retroviral; To be used in a tet-on…
    Tags
    myc
    Expression
    Mammalian
    Mutation
    fragment ([aa2-171]) of aquifex aeolicus BirA wit…
    Promoter
    TRE-cmv
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172878-plasmid-map-sequence-id-340526

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  33. pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

    Plasmid
    #141386
    Purpose
    Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-M (M SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 8, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141386-plasmid-map-sequence-id-288075

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  34. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid
    #138150
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Mutation
    DR1 A>T
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138150-plasmid-map-sequence-id-350242

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  39. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Publication
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62858-plasmid-map-sequence-id-243538

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Showing: 1361 - 1400 of 179521 results