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167,150 results

Showing: 1751 - 1800 of 167150 results

  1. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  2. tdTomato-Lysosomes-20

    Plasmid
    #58109
    Purpose
    Localization: Lysosome Membrane, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pPB-C6F/TdTomato

    Plasmid
    #140826
    Purpose
    OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)
    Depositor
    Hideyuki Okano
    Article
    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    C-MYC (MYC Human)
    KLF2 (KLF2 Human)
    NANOG (NANOG Human)
    tdTomato
    Expression
    Mammalian
    Mutation
    L104P- please see depositor comments
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    140826-plasmid-map-sequence-id-307041

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  8. pcDNA3.1-OGT_opt

    Plasmid
    #190858
    Purpose
    For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells.
    Promoter
    CMV
    Available Since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190858-plasmid-map-sequence-id-379243

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  17. pmVen-RPA70-IRES-RPA32-IRES-RPA14

    Plasmid
    #211431
    Purpose
    mammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14
    Depositor
    Helmut Pospiech
    Insert
    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    Tags
    mVenus (on RPA70)
    Expression
    Mammalian
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    211431-plasmid-map-sequence-id-420345

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  20. pLKO.3G

    Plasmid
    #14748
    Depositor
    Christophe Benoist, Diane Mathis
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14748-plasmid-map-sequence-id-352429

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  27. TFORF2165

    Plasmid
    #141953
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GRHL3 (GRHL3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    141953-plasmid-map-sequence-id-391431

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  29. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc

    Plasmid
    #193346
    Purpose
    tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc)
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Pou5f1, Sox2 Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    TetO mini CMV
    Available Since
    Feb. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    193346-plasmid-map-sequence-id-384485

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  31. TA1-DuET

    Plasmid
    #213382
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TA1 (TAAR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213382-plasmid-map-sequence-id-430737

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  35. EF1a_mCherry_P2A_Hygro

    Plasmid
    #135003
    Purpose
    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135003-plasmid-map-sequence-id-272070

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  36. pICH41331::pU6cm-pegR::EURb7

    Plasmid
    #227695
    Purpose
    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).
    Depositor
    Jae-Yean Kim
    Article
    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41
    Insert
    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    Use
    CRISPR
    Expression
    Plant
    Promoter
    U6 composite
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227695-plasmid-map-sequence-id-452432

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  44. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid
    #197507
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197507-plasmid-map-sequence-id-386698

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  46. TFORF0219

    Plasmid
    #142563
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ADNP (ADNP Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142563-plasmid-map-sequence-id-395965

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Showing: 1751 - 1800 of 167150 results