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163,486 results

Showing: 1751 - 1800 of 163486 results

  2. pYPQAT01

    Plasmid
    #223373
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223373-plasmid-map-sequence-id-454171

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  3. pMT-Ex

    Plasmid
    #231106
    Purpose
    Inducible protein expression in insect cell culture. Stable cell line generation.
    Depositor
    Michael Jeltsch
    Type
    Empty backbone
    Use
    Tags
    6xHis and V5
    Expression
    Insect
    Mutation
    Promoter
    Drosophila metallothionein promoter, MT
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    231106-plasmid-map-sequence-id-467321

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  6. DVK_p15A_AE

    Plasmid
    #217582
    Purpose
    Destination vector with kanamycin resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Article
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217582-plasmid-map-sequence-id-442592

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  7. LEGO-AP1x6-GM55

    Plasmid
    #217415
    Purpose
    Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    217415-plasmid-map-sequence-id-441062

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  8. pHR-mCh-Cry2WT_NLS

    Plasmid
    #221925
    Purpose
    Expression of SV40 nuclear localization signal (NLS) fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    SV40 NLS
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of SV40 NLS
    Promoter
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221925-plasmid-map-sequence-id-437713

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  10. pRPCN

    Plasmid
    #193726
    Purpose
    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193726-plasmid-map-sequence-id-381907

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  11. pDONR221-MFSD14A_STOP

    Plasmid
    #161035
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    MFSD14A (HIAT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161035-plasmid-map-sequence-id-312004

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  14. pLIX403-ccdB-G418

    Plasmid
    #158561
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158561-plasmid-map-sequence-id-309877

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  22. AICSDP-43: GJA1-mEGFP

    Plasmid
    #109120
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1
    Depositor
    Allen Institute for Cell Science
    Insert
    GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109120-plasmid-map-sequence-id-209766

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  23. pMKO.1 GFP

    Plasmid
    #10676
    Purpose
    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.
    Depositor
    William Hahn
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    10676-plasmid-map-sequence-id-139182

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  42. pKM512

    Plasmid
    #140192
    Purpose
    For curing of Orbit-integrated plasmids and associated drug marker
    Depositor
    Kenan Murphy
    Article
    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-
    Inserts
    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cloned in gp47 directinality factor in place of R…
    Promoter
    EM7 and Ptet
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    140192-plasmid-map-sequence-id-418051

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  43. PB-TA-ERN

    Plasmid
    #80474
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80474-plasmid-map-sequence-id-151580

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  48. pAAV.CMV.Luc.IRES.EGFP.SV40

    Plasmid
    #105533
    Purpose
    AAV expression of Luciferase and EGFP from CMV promoter
    Depositor
    James M. Wilson
    Insert
    Luciferase
    Use
    AAV and Luciferase
    Tags
    IRES-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105533-plasmid-map-sequence-id-202367

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Showing: 1751 - 1800 of 163486 results