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We narrowed to 18,759 results for: nls

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Showing: 2121 - 2160 of 18759 results

  3. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51761-plasmid-map-sequence-id-79377

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  5. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100936-plasmid-map-sequence-id-206124

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  12. pX330-EN1201

    Plasmid
    #92144
    Purpose
    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    Expression
    Mammalian
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92144-plasmid-map-sequence-id-177211

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  13. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid
    #78311
    Purpose
    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.
    Depositor
    Timo Otonkoski
    Publication
    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78311-plasmid-map-sequence-id-146188

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  18. pBT375

    Plasmid
    #125616
    Purpose
    Expresses evoAPOBEC1-BE4max-NG in mammalian cells
    Depositor
    David Liu
    Publication
    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    evoAPOBEC1-BE4max-NG
    Expression
    Mammalian
    Mutation
    E4K H109N H122L D124N R154H A165S P201S F205S
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    125616-plasmid-map-sequence-id-243546

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  19. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86613-plasmid-map-sequence-id-168851

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  25. pCMV-PE6a

    Plasmid
    #207851
    Purpose
    Mammalian expression of PE6a prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6a
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207851-plasmid-map-sequence-id-450977

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  26. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118154-plasmid-map-sequence-id-239465

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  28. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58694-plasmid-map-sequence-id-90691

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  35. Ezh2[FL]-dCas9

    Plasmid
    #100086
    Purpose
    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    14056 (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[FL]-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100086-plasmid-map-sequence-id-197550

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Showing: 2121 - 2160 of 18759 results