Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

Your Selections

50+ requests

8,626 results

Showing: 2701 - 2750 of 8626 results

  1. 3XMEF2-luc

    Plasmid
    #32967
    Purpose
    Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter
    Depositor
    Ron Prywes
    Insert
    MEF2 binding sites (MEF2A Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    MEF2
    Available since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    32967-plasmid-map-sequence-id-228104

    Enlarge

  3. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

    Enlarge

  4. pOpen_taq

    Plasmid
    #153315
    Purpose
    Bacterial expression of Taq polymerase
    Depositor
    Drew Endy
    Insert
    Taq polymerase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153315-plasmid-map-sequence-id-298774

    Enlarge

  10. pGL4.23-GW

    Plasmid
    #60323
    Purpose
    This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).
    Depositor
    Jorge Ferrer
    Article
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    60323-plasmid-map-sequence-id-316810

    Enlarge

  11. mCherry-LaminB1-10

    Plasmid
    #55069
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55069-plasmid-map-sequence-id-204489

    Enlarge

  18. HTR2A-Tango

    Plasmid
    #66409
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR2A (HTR2A Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66409-plasmid-map-sequence-id-244394

    Enlarge

  19. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185912-plasmid-map-sequence-id-367362

    Enlarge

  21. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139835-plasmid-map-sequence-id-310615

    Enlarge

  23. pEBFP2-Nuc

    Plasmid
    #14893
    Purpose
    Mammalian expression of EBFP2 blue fluorescent protein with NLS
    Depositor
    Robert Campbell
    Article
    Ai et al Biochemistry. 2007 Apr 20. ():.
    Insert
    pEBFP2-Nuc
    Use
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Promoter
    Available since
    May 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    14893-plasmid-map-sequence-id-122872

    Enlarge

  24. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid
    #52421
    Purpose
    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry
    Depositor
    Bertrand Collet
    Insert
    Fusion NLS-EGFP-P2A-Cherry-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    52421-plasmid-map-sequence-id-81252

    Enlarge

  27. pFlag-CMV1-hMD2

    Plasmid
    #13028
    Depositor
    Doug Golenbock
    Insert
    MD2 (LY96 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13028-plasmid-map-sequence-id-169271

    Enlarge

  28. pSynSRE-T-Luc

    Plasmid
    #60444
    Purpose
    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
    Depositor
    Timothy Osborne
    Article
    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.
    Insert
    HMG-CoA synthase promoter (-324/-225)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60444-plasmid-map-sequence-id-266122

    Enlarge

  30. PX852

    Plasmid
    #62884
    Purpose
    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    62884-plasmid-map-sequence-id-105906

    Enlarge

  31. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and hSyn
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60231-plasmid-map-sequence-id-417804

    Enlarge

  34. RelB cFlag pcDNA3

    Plasmid
    #20017
    Depositor
    Stephen Smale
    Insert
    RelB (Relb Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20017-plasmid-map-sequence-id-197332

    Enlarge

  35. CMV-ER-LAR-GECO1

    Plasmid
    #61244
    Purpose
    Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells
    Depositor
    Robert Campbell
    Article
    Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi
    Insert
    LAR-GECO1
    Use
    Tags
    ER-retention sequence: KDEL and ER-targeting sequ…
    Expression
    Mammalian
    Mutation
    Substitutions relative to R-GECO1: V51W/I113V/N35…
    Promoter
    CMV
    Available since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61244-plasmid-map-sequence-id-117077

    Enlarge

  38. pRK5-myc-RhoA-wt

    Plasmid
    #12962
    Depositor
    Gary Bokoch
    Insert
    RhoA (RHOA Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  47. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

    Plasmid
    #105543
    Purpose
    AAV expression of EGFP from cTNT promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    cTNT-PI-EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    cTNT
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105543-plasmid-map-sequence-id-202437

    Enlarge

  49. pCMV-15F11-HA-mCh

    Plasmid
    #129591
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129591-plasmid-map-sequence-id-254269

    Enlarge

Showing: 2701 - 2750 of 8626 results